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The map label for this gene is hemL [H]

Identifier: 21674908

GI number: 21674908

Start: 1990315

End: 1991610

Strand: Direct

Name: hemL [H]

Synonym: CT2099

Alternate gene names: 21674908

Gene position: 1990315-1991610 (Clockwise)

Preceding gene: 21674907

Following gene: 21674917

Centisome position: 92.36

GC content: 60.65

Gene sequence:

>1296_bases
ATGCCTGTACTTACCCGTTCTGCCGAGCTTTTTGAAAAAGCCAAAAAGTTCATCCCCGGTGGCGTCAACTCCCCAGTTCG
CGCCTTCAAATCCGTCGGTGGCACCCCGATCTACATGGCCAAGGGCCAGGGCGCTTACATGACCGACGTTGACGGCAACA
CCTACCTGGACTATGTCGGATCGTGGGGGCCGTTCATTCTCGGCAGCATGCATCCCCGCGTGACCGCCGCGATTGAATAC
ACCCTGCGCAATATCGGCACCAGCTTCGGCACGCCGATCGAACTCGAGATCGAAATCGCCGAGCTGCTTTGCAAGATCGT
GCCGTCGCTCGAAATGGTGCGCATGGTCAACAGCGGCACCGAAGCCACCATGTCCGCCGTCCGCCTCGCCCGCGGCTACA
CCGGCAAGGACAAGATCATCAAATTCGAGGGCTGCTACCACGGCCACGGCGACAGCTTCCTCATCAAGGCCGGTTCCGGC
GTGCTCACCCTCGGCGATCCCGACAGCCCGGGCGTCACCAAAGGTACCGCCAACGACACCCTCAACGCCACCTACAACGA
CATCGAGTCGGTCAAGGCGATCGTCAACGAGAACAAAGGCCAGGTCGCTGCAATCATCATCGAGCCGGTTGCTGGCAACA
CCGGCGTCATTCCGGCCAAGAAAGAGTTCCTCGTCGCCCTCCGCGAGCTGTGCGACGCTGAAGGCATCGTGCTGATTTTC
GACGAGGTGATGTGCGGTTTTCGTGTGGCCCTCGGCGGCGCTCAGGAGCTGTACGGCGTCACCCCTGACCTCACCACGAT
GGGCAAAATCATCGGCGGCGGTCTGCCGGTCGGCGCATTCGGCGGCAAACGCCACATCATGGAGAACATCGCTCCGCTCG
GCTCGGTCTATCAGGCAGGCACCCTCTCGGGCAACCCGCTCGCGCTGACCGCCGGTCTCGAAACCCTGAAAATCCTCATG
GAGGAGAATCCCTATCCGGAGCTCGAAAGAAAGGCTGCGTTCCTCGAAGCAGGATTCAAGGCCAACATGGAGAAGCTCGG
CCTGAACTACACGCAGAACCGCGTCGGTTCGATGGCCTGCCTCTTCTTCACCGAGACCCCGGTCGTGGACTACAAGAGCG
CCATCACCGCTGACACGGCCAAGTACGGCAAGTACTTCCACTCCATGCTCGACCAGGGCATCTACCTCGCCCCGTCGCAG
TTCGAGGCCATGTTCACCAGCTTCGCCCACACCGACGAGGATCTGGAGAAGACCGTCAAGGCAAACTACAACGCCCTCGT
CGCTGCTACCAAATAA

Upstream 100 bases:

>100_bases
GACATGCGGCATGCCTGTTTTCAATCTTCGGGACTATTTTCTATCTTGACCAGTCATTTAAAGCCATAACCCTCGACTAA
TTCAAACCGAACGATTCGAC

Downstream 100 bases:

>100_bases
GTTGCAAGAGGACGCACAGAAACGCAAAAGCGGCTATCTCGAATTACCGTGAGAGCCGCTTTTCCTTTTTCATAAAAAAC
CTTTGGTTCTTCGCAGAAGA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPVLTRSAELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRVTAAIEY
TLRNIGTSFGTPIELEIEIAELLCKIVPSLEMVRMVNSGTEATMSAVRLARGYTGKDKIIKFEGCYHGHGDSFLIKAGSG
VLTLGDPDSPGVTKGTANDTLNATYNDIESVKAIVNENKGQVAAIIIEPVAGNTGVIPAKKEFLVALRELCDAEGIVLIF
DEVMCGFRVALGGAQELYGVTPDLTTMGKIIGGGLPVGAFGGKRHIMENIAPLGSVYQAGTLSGNPLALTAGLETLKILM
EENPYPELERKAAFLEAGFKANMEKLGLNYTQNRVGSMACLFFTETPVVDYKSAITADTAKYGKYFHSMLDQGIYLAPSQ
FEAMFTSFAHTDEDLEKTVKANYNALVAATK

Sequences:

>Translated_431_residues
MPVLTRSAELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRVTAAIEY
TLRNIGTSFGTPIELEIEIAELLCKIVPSLEMVRMVNSGTEATMSAVRLARGYTGKDKIIKFEGCYHGHGDSFLIKAGSG
VLTLGDPDSPGVTKGTANDTLNATYNDIESVKAIVNENKGQVAAIIIEPVAGNTGVIPAKKEFLVALRELCDAEGIVLIF
DEVMCGFRVALGGAQELYGVTPDLTTMGKIIGGGLPVGAFGGKRHIMENIAPLGSVYQAGTLSGNPLALTAGLETLKILM
EENPYPELERKAAFLEAGFKANMEKLGLNYTQNRVGSMACLFFTETPVVDYKSAITADTAKYGKYFHSMLDQGIYLAPSQ
FEAMFTSFAHTDEDLEKTVKANYNALVAATK
>Mature_430_residues
PVLTRSAELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRVTAAIEYT
LRNIGTSFGTPIELEIEIAELLCKIVPSLEMVRMVNSGTEATMSAVRLARGYTGKDKIIKFEGCYHGHGDSFLIKAGSGV
LTLGDPDSPGVTKGTANDTLNATYNDIESVKAIVNENKGQVAAIIIEPVAGNTGVIPAKKEFLVALRELCDAEGIVLIFD
EVMCGFRVALGGAQELYGVTPDLTTMGKIIGGGLPVGAFGGKRHIMENIAPLGSVYQAGTLSGNPLALTAGLETLKILME
ENPYPELERKAAFLEAGFKANMEKLGLNYTQNRVGSMACLFFTETPVVDYKSAITADTAKYGKYFHSMLDQGIYLAPSQF
EAMFTSFAHTDEDLEKTVKANYNALVAATK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=324, Percent_Identity=30.8641975308642, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI4557809, Length=304, Percent_Identity=30.5921052631579, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI37574042, Length=330, Percent_Identity=28.7878787878788, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI24119277, Length=305, Percent_Identity=27.5409836065574, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI226442705, Length=331, Percent_Identity=29.0030211480363, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI226442709, Length=299, Percent_Identity=28.4280936454849, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI284507298, Length=223, Percent_Identity=32.7354260089686, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1786349, Length=420, Percent_Identity=54.5238095238095, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1789016, Length=353, Percent_Identity=32.5779036827195, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1789759, Length=304, Percent_Identity=33.5526315789474, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788044, Length=297, Percent_Identity=33.6700336700337, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787560, Length=299, Percent_Identity=33.1103678929766, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693181, Length=305, Percent_Identity=30.8196721311475, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786991, Length=311, Percent_Identity=28.9389067524116, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71992977, Length=371, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=325, Percent_Identity=29.5384615384615, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=35.6643356643357, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=332, Percent_Identity=28.3132530120482, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=325, Percent_Identity=25.2307692307692, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI161085790, Length=339, Percent_Identity=28.9085545722714, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574759, Length=339, Percent_Identity=28.9085545722714, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356575, Length=322, Percent_Identity=29.8136645962733, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357415, Length=302, Percent_Identity=28.1456953642384, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46184; Mature: 46052

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLTRSAELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVG
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEHCCC
SWGPFILGSMHPRVTAAIEYTLRNIGTSFGTPIELEIEIAELLCKIVPSLEMVRMVNSGT
CCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCH
EATMSAVRLARGYTGKDKIIKFEGCYHGHGDSFLIKAGSGVLTLGDPDSPGVTKGTANDT
HHHHHHHHHHCCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LNATYNDIESVKAIVNENKGQVAAIIIEPVAGNTGVIPAKKEFLVALRELCDAEGIVLIF
CCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
DEVMCGFRVALGGAQELYGVTPDLTTMGKIIGGGLPVGAFGGKRHIMENIAPLGSVYQAG
HHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TLSGNPLALTAGLETLKILMEENPYPELERKAAFLEAGFKANMEKLGLNYTQNRVGSMAC
CCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHCCCHHHHHHCCEEE
LFFTETPVVDYKSAITADTAKYGKYFHSMLDQGIYLAPSQFEAMFTSFAHTDEDLEKTVK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHHH
ANYNALVAATK
CCCCEEEEECC
>Mature Secondary Structure 
PVLTRSAELFEKAKKFIPGGVNSPVRAFKSVGGTPIYMAKGQGAYMTDVDGNTYLDYVG
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEHCCC
SWGPFILGSMHPRVTAAIEYTLRNIGTSFGTPIELEIEIAELLCKIVPSLEMVRMVNSGT
CCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCH
EATMSAVRLARGYTGKDKIIKFEGCYHGHGDSFLIKAGSGVLTLGDPDSPGVTKGTANDT
HHHHHHHHHHCCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LNATYNDIESVKAIVNENKGQVAAIIIEPVAGNTGVIPAKKEFLVALRELCDAEGIVLIF
CCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
DEVMCGFRVALGGAQELYGVTPDLTTMGKIIGGGLPVGAFGGKRHIMENIAPLGSVYQAG
HHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TLSGNPLALTAGLETLKILMEENPYPELERKAAFLEAGFKANMEKLGLNYTQNRVGSMAC
CCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHCCCHHHHHHCCEEE
LFFTETPVVDYKSAITADTAKYGKYFHSMLDQGIYLAPSQFEAMFTSFAHTDEDLEKTVK
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHHH
ANYNALVAATK
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA