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The map label for this gene is hsdS-2 [H]

Identifier: 21674692

GI number: 21674692

Start: 1778970

End: 1780394

Strand: Reverse

Name: hsdS-2 [H]

Synonym: CT1880

Alternate gene names: 21674692

Gene position: 1780394-1778970 (Counterclockwise)

Preceding gene: 21674693

Following gene: 21674691

Centisome position: 82.62

GC content: 52.63

Gene sequence:

>1425_bases
ATGAGCGGCAGGGAGTTTTTGCAGAAGCTGCTGGATGGGGAGCGAGTGGAGTGGAAGGCGCTGGGGGAGATTATTCAACT
GGAAAAAGGCAGGCAGTTAAATAAGGATCTGCTCTCTTCTAGCGGTCGCTACCCAGCATATAACGGTGGAATGTCTTACT
CTGGCTTTACCGACAGTTATAACTATAGCGAAAATAAAACAATTATTAGCCAGGGTGGCGCCTCCGCAGGTTTTGTGAAC
TTCGTTACCACGAAGTTCTACGCAAATGCGCACTGCTATGTGGTGCTGCCCGATACCGAAGTAGTCGACAATCGCTACAT
CTACCATTTCCTAAAACTGAATGAGGAAAGACTAACCAGCTGCCAGCATGGTGCAGGAATACCAGCGTTACGAGCGAGCG
AAATAACTAGTCTCAAAATCCCCATCCCCTGCCCCGACAATCCTAAAAAATCGCTGGCCATCCAGGCTGAAATCGTCCGC
ATTCTGGACGCCTTCACCGAGCTGACCGCCGAGCTGACCGCCGAGCTGACCGCCCGCAAAAAACAATATGCTTACTACCG
GGATCGGTTGTTGACTTTTACCACCCCGCCCTACGGGCACCCCTCCAAAGGAGGGGAACTGTTCTCGCTCTTCGGGCACC
CCTCCGAGGGAGGGGAATTGTTCACGCCCTACGGGCACTCTGTTGAGGAAAGAGAATTAAATTCCCCTTCTCTGAAGGGG
TGGCAGGCGCAGCCTGACGGGGTAGTTCCTGTGGAGTGGAAGACGTTGGGGGAGGTTGGTCATTTTATTCGTGGTTCTGG
AATTCAGAAATCTGACTTCAAAGCCTCCGGCGTTGGATGCATTCACTACGGGCAGATTCATACGCATTACGGCACATGGA
CCACCGAAACCAAGTCGTTCATTGATCCAGAGTTTGCAAATCGGCTAAAAAAGGCAAAACCCGGCGATCTGGTCATTGCC
ACAACGAGCGAAGACGATGATGCGGTTGCCAAAGCTGTCGCATGGATAGGAACTGAGGATGTCGCCGTCAGCACCGACGC
TTATATTTTTCGGCATACGGCAAACCCCAAATACATGTCCTATTTTTTTCAAACGGATATGTTTCAGGAGCAAAAGAAGC
CTTACATCACCGGAACAAAGGTACGTCGCATATCTGGCGACAATCTGGCAAAAATCCTAATCCCCATCCCACCACTCGCC
GAGCAAGAGCGCATCGTTGCAATCCTCGACCAATTCGACGCCCTCACCAACTCCCTTACCGAGGGGCTGCCGCGCGAGAT
CGAACTGCGGCAGAAGCAATACGCCTACTACCGCGATCTGCTGTTCAGCTTCCCCAAAGCATCTTTTGGAGGGGTGCCCG
AAGGGCGGGATCAATTCCCCTCCTTTGGAGGGGTGCCCGAAGGGCGGGGTGGTCTCAATGCGTAA

Upstream 100 bases:

>100_bases
TGCCTTTGTTCTACAAACTGGCCGCGGCGCTTCCTGGCCCGCTCAAGACCAGCCTGCCCAGCATCGAGCAGATCGAGCGC
GAATTGCAAGGAGGTGCAGC

Downstream 100 bases:

>100_bases
AACCCAAACCTACAAATCCTTACCCTATAACCCTGCTTTAAGGGATAGGGCAAAAGCCTTACGTAAAGCGGGTATATTGC
ACGAAGCCTTACTTTGGTTT

Product: type I restriction system specificity protein

Products: NA

Alternate protein names: S.EcoR124II; Type I restriction enzyme EcoR124II specificity protein; S protein [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSGREFLQKLLDGERVEWKALGEIIQLEKGRQLNKDLLSSSGRYPAYNGGMSYSGFTDSYNYSENKTIISQGGASAGFVN
FVTTKFYANAHCYVVLPDTEVVDNRYIYHFLKLNEERLTSCQHGAGIPALRASEITSLKIPIPCPDNPKKSLAIQAEIVR
ILDAFTELTAELTAELTARKKQYAYYRDRLLTFTTPPYGHPSKGGELFSLFGHPSEGGELFTPYGHSVEERELNSPSLKG
WQAQPDGVVPVEWKTLGEVGHFIRGSGIQKSDFKASGVGCIHYGQIHTHYGTWTTETKSFIDPEFANRLKKAKPGDLVIA
TTSEDDDAVAKAVAWIGTEDVAVSTDAYIFRHTANPKYMSYFFQTDMFQEQKKPYITGTKVRRISGDNLAKILIPIPPLA
EQERIVAILDQFDALTNSLTEGLPREIELRQKQYAYYRDLLFSFPKASFGGVPEGRDQFPSFGGVPEGRGGLNA

Sequences:

>Translated_474_residues
MSGREFLQKLLDGERVEWKALGEIIQLEKGRQLNKDLLSSSGRYPAYNGGMSYSGFTDSYNYSENKTIISQGGASAGFVN
FVTTKFYANAHCYVVLPDTEVVDNRYIYHFLKLNEERLTSCQHGAGIPALRASEITSLKIPIPCPDNPKKSLAIQAEIVR
ILDAFTELTAELTAELTARKKQYAYYRDRLLTFTTPPYGHPSKGGELFSLFGHPSEGGELFTPYGHSVEERELNSPSLKG
WQAQPDGVVPVEWKTLGEVGHFIRGSGIQKSDFKASGVGCIHYGQIHTHYGTWTTETKSFIDPEFANRLKKAKPGDLVIA
TTSEDDDAVAKAVAWIGTEDVAVSTDAYIFRHTANPKYMSYFFQTDMFQEQKKPYITGTKVRRISGDNLAKILIPIPPLA
EQERIVAILDQFDALTNSLTEGLPREIELRQKQYAYYRDLLFSFPKASFGGVPEGRDQFPSFGGVPEGRGGLNA
>Mature_473_residues
SGREFLQKLLDGERVEWKALGEIIQLEKGRQLNKDLLSSSGRYPAYNGGMSYSGFTDSYNYSENKTIISQGGASAGFVNF
VTTKFYANAHCYVVLPDTEVVDNRYIYHFLKLNEERLTSCQHGAGIPALRASEITSLKIPIPCPDNPKKSLAIQAEIVRI
LDAFTELTAELTAELTARKKQYAYYRDRLLTFTTPPYGHPSKGGELFSLFGHPSEGGELFTPYGHSVEERELNSPSLKGW
QAQPDGVVPVEWKTLGEVGHFIRGSGIQKSDFKASGVGCIHYGQIHTHYGTWTTETKSFIDPEFANRLKKAKPGDLVIAT
TSEDDDAVAKAVAWIGTEDVAVSTDAYIFRHTANPKYMSYFFQTDMFQEQKKPYITGTKVRRISGDNLAKILIPIPPLAE
QERIVAILDQFDALTNSLTEGLPREIELRQKQYAYYRDLLFSFPKASFGGVPEGRDQFPSFGGVPEGRGGLNA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 52779; Mature: 52648

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGREFLQKLLDGERVEWKALGEIIQLEKGRQLNKDLLSSSGRYPAYNGGMSYSGFTDSY
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCC
NYSENKTIISQGGASAGFVNFVTTKFYANAHCYVVLPDTEVVDNRYIYHFLKLNEERLTS
CCCCCCEEEECCCCCCCHHHHEEHEEEECCEEEEEECCCHHHCCCEEEEEEECCHHHHHH
CQHGAGIPALRASEITSLKIPIPCPDNPKKSLAIQAEIVRILDAFTELTAELTAELTARK
HHCCCCCCCEECCCCCEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQYAYYRDRLLTFTTPPYGHPSKGGELFSLFGHPSEGGELFTPYGHSVEERELNSPSLKG
HHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCEECCCCCCCHHHCCCCCCCCC
WQAQPDGVVPVEWKTLGEVGHFIRGSGIQKSDFKASGVGCIHYGQIHTHYGTWTTETKSF
CCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECEEEEECCCEECCCHHH
IDPEFANRLKKAKPGDLVIATTSEDDDAVAKAVAWIGTEDVAVSTDAYIFRHTANPKYMS
CCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHHH
YFFQTDMFQEQKKPYITGTKVRRISGDNLAKILIPIPPLAEQERIVAILDQFDALTNSLT
HHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
EGLPREIELRQKQYAYYRDLLFSFPKASFGGVPEGRDQFPSFGGVPEGRGGLNA
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGREFLQKLLDGERVEWKALGEIIQLEKGRQLNKDLLSSSGRYPAYNGGMSYSGFTDSY
CHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCC
NYSENKTIISQGGASAGFVNFVTTKFYANAHCYVVLPDTEVVDNRYIYHFLKLNEERLTS
CCCCCCEEEECCCCCCCHHHHEEHEEEECCEEEEEECCCHHHCCCEEEEEEECCHHHHHH
CQHGAGIPALRASEITSLKIPIPCPDNPKKSLAIQAEIVRILDAFTELTAELTAELTARK
HHCCCCCCCEECCCCCEEEEECCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQYAYYRDRLLTFTTPPYGHPSKGGELFSLFGHPSEGGELFTPYGHSVEERELNSPSLKG
HHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCEECCCCCCCHHHCCCCCCCCC
WQAQPDGVVPVEWKTLGEVGHFIRGSGIQKSDFKASGVGCIHYGQIHTHYGTWTTETKSF
CCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECEEEEECCCEECCCHHH
IDPEFANRLKKAKPGDLVIATTSEDDDAVAKAVAWIGTEDVAVSTDAYIFRHTANPKYMS
CCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHHH
YFFQTDMFQEQKKPYITGTKVRRISGDNLAKILIPIPPLAEQERIVAILDQFDALTNSLT
HHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
EGLPREIELRQKQYAYYRDLLFSFPKASFGGVPEGRDQFPSFGGVPEGRGGLNA
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]