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The map label for this gene is ispF

Identifier: 21674419

GI number: 21674419

Start: 1508725

End: 1509198

Strand: Direct

Name: ispF

Synonym: CT1601

Alternate gene names: 21674419

Gene position: 1508725-1509198 (Clockwise)

Preceding gene: 21674418

Following gene: 21674421

Centisome position: 70.01

GC content: 58.86

Gene sequence:

>474_bases
ATGCGTATAGGAATCGGCATAGACGTTCACCAGTTTGCAGAAGGCCGGAAACTCATCATCGGCGGAGTTGAAGTGCCCTC
CCCGATCGGACTCCTCGGCCACAGCGATGCCGATGTGCTGCTGCACGCCATCAGCGACGCCCTGCTCGGCGCGGCAGCTC
TTGGGGACATCGGCAAGCACTTCCCCGACACCAGCCCCGACTACAAAGATGCAGACAGCATGGAGCTGCTGAGGCATGTC
TGCAAGCTTCTTGAACAGGAAGGCTACAAGCCGGTCAACGTCGATACCATGCTGCTGCTCGAAAAACCGAAAATCGCTCC
CTACATCGACCAGATGCGCCGCAACATCGCCCGCTGCCTCGGCCTGGAGATAAACGCCGTGTCGGTCAAAGCAACCACCA
ACGAAAAGCTCGGCTATGTAGGCCGTCAGGAGGGTGCCTGCGCCCATGCGGTGTGCCTGATTGAAAATGCCTGA

Upstream 100 bases:

>100_bases
TTTCAAATTGGAACGAAAGGTTCTGTATTTACCCGTTTTGTTCTATCTTTCAGCCCATATCGCTGCATAGACACCAATCC
ACCACCAATTTACAGAGAAC

Downstream 100 bases:

>100_bases
ATGAACGCATGTTTTCCGCGTCGTGAATGGCTTGATGACAAGGCTGACGAAGCACGGCAAGCGGGACGCACAACAGAGGA
AAACATGCAGTTTACCCCAC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGIGIDVHQFAEGRKLIIGGVEVPSPIGLLGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDYKDADSMELLRHV
CKLLEQEGYKPVNVDTMLLLEKPKIAPYIDQMRRNIARCLGLEINAVSVKATTNEKLGYVGRQEGACAHAVCLIENA

Sequences:

>Translated_157_residues
MRIGIGIDVHQFAEGRKLIIGGVEVPSPIGLLGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDYKDADSMELLRHV
CKLLEQEGYKPVNVDTMLLLEKPKIAPYIDQMRRNIARCLGLEINAVSVKATTNEKLGYVGRQEGACAHAVCLIENA
>Mature_157_residues
MRIGIGIDVHQFAEGRKLIIGGVEVPSPIGLLGHSDADVLLHAISDALLGAAALGDIGKHFPDTSPDYKDADSMELLRHV
CKLLEQEGYKPVNVDTMLLLEKPKIAPYIDQMRRNIARCLGLEINAVSVKATTNEKLGYVGRQEGACAHAVCLIENA

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=55.4140127388535, Blast_Score=157, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CHLTE (Q8KC25)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662484.1
- ProteinModelPortal:   Q8KC25
- SMR:   Q8KC25
- GeneID:   1006100
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1601
- NMPDR:   fig|194439.1.peg.1578
- TIGR:   CT1601
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   CTEP194439:CT_1601-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16924; Mature: 16924

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIGIDVHQFAEGRKLIIGGVEVPSPIGLLGHSDADVLLHAISDALLGAAALGDIGKH
CEECCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTSPDYKDADSMELLRHVCKLLEQEGYKPVNVDTMLLLEKPKIAPYIDQMRRNIARCL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
GLEINAVSVKATTNEKLGYVGRQEGACAHAVCLIENA
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRIGIGIDVHQFAEGRKLIIGGVEVPSPIGLLGHSDADVLLHAISDALLGAAALGDIGKH
CEECCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPDTSPDYKDADSMELLRHVCKLLEQEGYKPVNVDTMLLLEKPKIAPYIDQMRRNIARCL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
GLEINAVSVKATTNEKLGYVGRQEGACAHAVCLIENA
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901