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The map label for this gene is pfk-1 [H]

Identifier: 21674421

GI number: 21674421

Start: 1510727

End: 1511932

Strand: Direct

Name: pfk-1 [H]

Synonym: CT1603

Alternate gene names: 21674421

Gene position: 1510727-1511932 (Clockwise)

Preceding gene: 21674419

Following gene: 21674422

Centisome position: 70.11

GC content: 59.45

Gene sequence:

>1206_bases
GTGAAAAAAATCGGAATTCTTACCAGCGGTGGCGACTGCGGCGGCCTGAACGCCGTCATCAAGGGTGCCGCACTCATGGC
CCTGAACAAGGGGCTTGAACTGTATGTGATCCCCAACGGCTACGCCGGACTGTACAACCTCCTGAATCAGGACCGGTTGG
TCAGGCTCGACGAGGCCCGTCTCGACCAGTTCCAGGCCAGCTTCGCCGGCTCGGAAGCCGGCCACTCGCGGGTGAAAATC
AAGGCGATCAGCAACCCCGACAAATACAACCGCATCAAGGTCGGCATGAAAAAGTTCGATCTCGACGCGCTCATCATCAG
CGGCGGCGACGACTCCGGCAGCGTCATGATCGACCTGAACCACAACGGCATCCAGTGCATCCACTGTCCAAAGACGATGG
ATCTTGACTTGCAGACCTACTCGGTCGGCGGTGACTCGACCATCAACCGCATCGCCCAGTTCGTCGAGGATCTGAAGACG
ACAGGACGTACCCACAACCGCGTGCTGGTCACGGAGGTGTTCGGACGCTACGCGGGCCACACGGCCTTCCGCAGCGGCGT
GGCTGCCGAAGCCGACTGCATCCTGATTCCGGAAATCCCGGTTGACTGGGATGTCGTGTACGAGCACATCGTCGAACGCT
TCACCCGCCGCATCCGGCAGAGCGACGTGCACAGCGGCACCTATACCATCGTGGTCGCCGAGGGCATGAAGAACGCCGAC
GGCACGGACATCGTCGATGAGTCGGCAGGCATCGATGCATTTGGCCACAAAAAGCTCGCGGGCGCGGGCAAGTATGTTTG
CCAGGAGATCAAGAAGCGCTTCAAGACCGACCCGCGAATGCCGCAGTTCATGAAGGATACCGGCATGTTCGTCGAGGGCA
TCTACGAAATTCCGGAGGTGCGCGAAGTCCATCCGGGCCACTTGGTGCGCGCCGGGCACTCCTCGGCCTACGACATCAAC
TTCGGCTTCGAGGCTGGCGCAGCAGCGGTATTGCTCCTGCTTGAAGGCAAAACCGGTGTGACGATCTCCAAGGTCAAGGG
GCGCAAAATCGAGTACATCGAAGCCTCAAAGGCGATCGAACAGCGCTATGTCGATCTCGACCAGGTTGCCATGTACGAAT
CGATCGGCACCTGTTTCGGCAGAACTCCGGCAGCCTACGAGCCGATCCTGCGAGAAGTTGACGGCGTGTACGAAAGGATT
TATTGA

Upstream 100 bases:

>100_bases
AATACTCTATCTTGATTTCATACCGTACACCATGCAACAATGGTGTAATAAATCAAATTAAGCTGTCAAAAACTAACTTG
AGGAACGAGGAGGGAGCAGC

Downstream 100 bases:

>100_bases
GTGCAAACGGCCTGAGTGGGCGCTGCGGACGAACCGCGTTCACTCAGGCCATCAAGCCCGTTCCACTCTATCGTCCGCGC
TCAAATGAGCTCCTCGCCGC

Product: phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKIGILTSGGDCGGLNAVIKGAALMALNKGLELYVIPNGYAGLYNLLNQDRLVRLDEARLDQFQASFAGSEAGHSRVKI
KAISNPDKYNRIKVGMKKFDLDALIISGGDDSGSVMIDLNHNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKT
TGRTHNRVLVTEVFGRYAGHTAFRSGVAAEADCILIPEIPVDWDVVYEHIVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
GTDIVDESAGIDAFGHKKLAGAGKYVCQEIKKRFKTDPRMPQFMKDTGMFVEGIYEIPEVREVHPGHLVRAGHSSAYDIN
FGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIEASKAIEQRYVDLDQVAMYESIGTCFGRTPAAYEPILREVDGVYERI
Y

Sequences:

>Translated_401_residues
MKKIGILTSGGDCGGLNAVIKGAALMALNKGLELYVIPNGYAGLYNLLNQDRLVRLDEARLDQFQASFAGSEAGHSRVKI
KAISNPDKYNRIKVGMKKFDLDALIISGGDDSGSVMIDLNHNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKT
TGRTHNRVLVTEVFGRYAGHTAFRSGVAAEADCILIPEIPVDWDVVYEHIVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
GTDIVDESAGIDAFGHKKLAGAGKYVCQEIKKRFKTDPRMPQFMKDTGMFVEGIYEIPEVREVHPGHLVRAGHSSAYDIN
FGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIEASKAIEQRYVDLDQVAMYESIGTCFGRTPAAYEPILREVDGVYERI
Y
>Mature_401_residues
MKKIGILTSGGDCGGLNAVIKGAALMALNKGLELYVIPNGYAGLYNLLNQDRLVRLDEARLDQFQASFAGSEAGHSRVKI
KAISNPDKYNRIKVGMKKFDLDALIISGGDDSGSVMIDLNHNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKT
TGRTHNRVLVTEVFGRYAGHTAFRSGVAAEADCILIPEIPVDWDVVYEHIVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
GTDIVDESAGIDAFGHKKLAGAGKYVCQEIKKRFKTDPRMPQFMKDTGMFVEGIYEIPEVREVHPGHLVRAGHSSAYDIN
FGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIEASKAIEQRYVDLDQVAMYESIGTCFGRTPAAYEPILREVDGVYERI
Y

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=370, Percent_Identity=25.1351351351351, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1790350, Length=349, Percent_Identity=28.9398280802292, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17558788, Length=418, Percent_Identity=26.0765550239234, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652337, Length=433, Percent_Identity=24.4803695150115, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17647809, Length=433, Percent_Identity=24.4803695150115, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28573326, Length=430, Percent_Identity=23.4883720930233, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 44093; Mature: 44093

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGILTSGGDCGGLNAVIKGAALMALNKGLELYVIPNGYAGLYNLLNQDRLVRLDEAR
CCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHHHHHHCCCCEEEECHHH
LDQFQASFAGSEAGHSRVKIKAISNPDKYNRIKVGMKKFDLDALIISGGDDSGSVMIDLN
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEECCEEECEEEEEEECCCCCCEEEEEEC
HNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKTTGRTHNRVLVTEVFGRYAGH
CCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGVAAEADCILIPEIPVDWDVVYEHIVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GTDIVDESAGIDAFGHKKLAGAGKYVCQEIKKRFKTDPRMPQFMKDTGMFVEGIYEIPEV
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHCCCCH
REVHPGHLVRAGHSSAYDINFGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIEASKAIE
HCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEHHHHHHH
QRYVDLDQVAMYESIGTCFGRTPAAYEPILREVDGVYERIY
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGILTSGGDCGGLNAVIKGAALMALNKGLELYVIPNGYAGLYNLLNQDRLVRLDEAR
CCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHHHHHHCCCCEEEECHHH
LDQFQASFAGSEAGHSRVKIKAISNPDKYNRIKVGMKKFDLDALIISGGDDSGSVMIDLN
HHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEECCEEECEEEEEEECCCCCCEEEEEEC
HNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKTTGRTHNRVLVTEVFGRYAGH
CCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGVAAEADCILIPEIPVDWDVVYEHIVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GTDIVDESAGIDAFGHKKLAGAGKYVCQEIKKRFKTDPRMPQFMKDTGMFVEGIYEIPEV
CCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHCCCCH
REVHPGHLVRAGHSSAYDINFGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIEASKAIE
HCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEHHHHHHH
QRYVDLDQVAMYESIGTCFGRTPAAYEPILREVDGVYERIY
HHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]