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The map label for this gene is nifN [H]

Identifier: 21674358

GI number: 21674358

Start: 1442183

End: 1443550

Strand: Direct

Name: nifN [H]

Synonym: CT1539

Alternate gene names: 21674358

Gene position: 1442183-1443550 (Clockwise)

Preceding gene: 21674357

Following gene: 21674359

Centisome position: 66.92

GC content: 60.45

Gene sequence:

>1368_bases
ATGACGACCAACGCTTCGGCCATGACGGCCAAAACGGCCACGCAGAACGCCTGCAAGCTCTGCACTCCGCTCGGCGCGTG
CCTGGCTTTCAGGGGCATCGAATCGTGTGTACCGTTCCTGCACGGCTCGCAGGGTTGCGCCACCTACATCCGCCGCTACC
TCATCAGCCACTACAAGGAGCCGATCGACATCGCCTCCTCGAACTTCAACGAAGAGACGGCGGTCTTCGGCGGCAGCCAC
AACCTGCAGCTCGGCCTCAAGAACGTCACGCAGCAGTACAAGCCACAGGTGATCGGCATCGCCACCACCTGTCTGTCGGA
GACCATCGGTGACGACGTGCCGATGATTCTGAAGGATTACAAGGCGATCATGAACGACCCCAACCTGCCGACCATGATCT
TCGCCTCGACGCCGAGTTACAGCGGCAGCCACATCGACGGCTTCCACACCGCCGTGCGCTCCGCCGTAAAAACTTTTGCC
GTCGGCGGGGCGAAAAAGAACCTGCTCAACCTCTTTTCGGGAATGATTTCACCGGCGGATATCCGCTACCTGAAGGAGAT
TCTGAAAGAGTTCGGCATGCCGTTCATGCTGCTGCCCGACTACTCGCAGACGCTTGACGGCGGTCCGTGGGGCGAGTACC
ACCGCATTCCGCCAGGCGGCACGCCGACGAGCGCTATCGCCGACAGCGGCAGCGCTGCAGCAAGCATCGAGTTCGGCTCG
ACGCTCGAAGCAAAGAAGTCTGCGGCTGGCTACCTCGAAGCAGAGTTTGGCGTTCCACGCCACCAGCTCCCGTTGCCGAT
CGGCATCAAGGCAACCGACCGTTTCTTCGCACTGCTCGAAGAGCTGACCGAAAAACCGATGCCTGAAAAATACGAAGACG
AGCGCCGCCGCCTCGTTGACGCCTACGCCGATGGCCACAAATACATCTTCGGCAGAAAGGCGATGCTCTACGGCGAGGAA
GACCTGGTCATCTCGATGGCCGCTTTCCTGCGAGAAATCGGCGTCGTGCCAGTGCTCTGCGCCTCGGGGGGCAAGAGCGG
CCAGATGAAGCAGCGGATGCTTGAGCTGATTCCCGACATGGATGAGCAGGGCATCGAAGCGTGCGAGGGCGTGGACTTCG
TGGACATCGAGCACGAAGCGGAGCGGCTCAAGCCGGATATGCTCATCGGCAACAGCAAGGGCTTCACGATGTCAAAAAAG
CACGAGCTGCCGCTCATCAGAATCGGCTTCCCGATTCACGACCGCTTCGGCGGCCAGCGCCTGCACCACCTCGGCTATCG
CGGTACGCAGGAGCTGTTCGACCGCATCGTCAACACCGTCATCGAAGAACGCCAGAAATCGTCGCCGATCGGCTACACCT
ATATGTAA

Upstream 100 bases:

>100_bases
CGCTCGCCGGATTCATCGGCATGTACTATTTCGCCAAGGAGGTTTACGAATCGGTGATGAGCCCCGTGTGGCAGTTCGCC
CCGAGAAAAGGAGGTGCAAA

Downstream 100 bases:

>100_bases
TGAAGAATCAATCATGACCTGAAGGGAGGCATCATGACACTCAATATCAAGAACCATCCGTGCTTCAACGACTCGTCGAG
ACACACCTACGGAAGAATAC

Product: nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, putative

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTTNASAMTAKTATQNACKLCTPLGACLAFRGIESCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSH
NLQLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDYKAIMNDPNLPTMIFASTPSYSGSHIDGFHTAVRSAVKTFA
VGGAKKNLLNLFSGMISPADIRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
TLEAKKSAAGYLEAEFGVPRHQLPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVDAYADGHKYIFGRKAMLYGEE
DLVISMAAFLREIGVVPVLCASGGKSGQMKQRMLELIPDMDEQGIEACEGVDFVDIEHEAERLKPDMLIGNSKGFTMSKK
HELPLIRIGFPIHDRFGGQRLHHLGYRGTQELFDRIVNTVIEERQKSSPIGYTYM

Sequences:

>Translated_455_residues
MTTNASAMTAKTATQNACKLCTPLGACLAFRGIESCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSH
NLQLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDYKAIMNDPNLPTMIFASTPSYSGSHIDGFHTAVRSAVKTFA
VGGAKKNLLNLFSGMISPADIRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
TLEAKKSAAGYLEAEFGVPRHQLPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVDAYADGHKYIFGRKAMLYGEE
DLVISMAAFLREIGVVPVLCASGGKSGQMKQRMLELIPDMDEQGIEACEGVDFVDIEHEAERLKPDMLIGNSKGFTMSKK
HELPLIRIGFPIHDRFGGQRLHHLGYRGTQELFDRIVNTVIEERQKSSPIGYTYM
>Mature_454_residues
TTNASAMTAKTATQNACKLCTPLGACLAFRGIESCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSHN
LQLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDYKAIMNDPNLPTMIFASTPSYSGSHIDGFHTAVRSAVKTFAV
GGAKKNLLNLFSGMISPADIRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGST
LEAKKSAAGYLEAEFGVPRHQLPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVDAYADGHKYIFGRKAMLYGEED
LVISMAAFLREIGVVPVLCASGGKSGQMKQRMLELIPDMDEQGIEACEGVDFVDIEHEAERLKPDMLIGNSKGFTMSKKH
ELPLIRIGFPIHDRFGGQRLHHLGYRGTQELFDRIVNTVIEERQKSSPIGYTYM

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50097; Mature: 49966

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNASAMTAKTATQNACKLCTPLGACLAFRGIESCVPFLHGSQGCATYIRRYLISHYKE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
PIDIASSNFNEETAVFGGSHNLQLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDY
CHHHHCCCCCCCCEEECCCCCEEECHHHHHHHHCCCEEHHHHHHHHHHHCCCCHHHHHHH
KAIMNDPNLPTMIFASTPSYSGSHIDGFHTAVRSAVKTFAVGGAKKNLLNLFSGMISPAD
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHH
IRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
HHHHHHHHHHHCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCC
TLEAKKSAAGYLEAEFGVPRHQLPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVD
CHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AYADGHKYIFGRKAMLYGEEDLVISMAAFLREIGVVPVLCASGGKSGQMKQRMLELIPDM
HHHCCCEEEEECEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCC
DEQGIEACEGVDFVDIEHEAERLKPDMLIGNSKGFTMSKKHELPLIRIGFPIHDRFGGQR
CHHHHHHHCCCCEEECCCHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCHHHHCCCHH
LHHLGYRGTQELFDRIVNTVIEERQKSSPIGYTYM
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTNASAMTAKTATQNACKLCTPLGACLAFRGIESCVPFLHGSQGCATYIRRYLISHYKE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
PIDIASSNFNEETAVFGGSHNLQLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDY
CHHHHCCCCCCCCEEECCCCCEEECHHHHHHHHCCCEEHHHHHHHHHHHCCCCHHHHHHH
KAIMNDPNLPTMIFASTPSYSGSHIDGFHTAVRSAVKTFAVGGAKKNLLNLFSGMISPAD
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHH
IRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
HHHHHHHHHHHCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCC
TLEAKKSAAGYLEAEFGVPRHQLPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVD
CHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AYADGHKYIFGRKAMLYGEEDLVISMAAFLREIGVVPVLCASGGKSGQMKQRMLELIPDM
HHHCCCEEEEECEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCC
DEQGIEACEGVDFVDIEHEAERLKPDMLIGNSKGFTMSKKHELPLIRIGFPIHDRFGGQR
CHHHHHHHCCCCEEECCCHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCHHHHCCCHH
LHHLGYRGTQELFDRIVNTVIEERQKSSPIGYTYM
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550408; 7961410 [H]