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The map label for this gene is nifB [H]

Identifier: 21674359

GI number: 21674359

Start: 1443584

End: 1444858

Strand: Direct

Name: nifB [H]

Synonym: CT1540

Alternate gene names: 21674359

Gene position: 1443584-1444858 (Clockwise)

Preceding gene: 21674358

Following gene: 21674360

Centisome position: 66.99

GC content: 59.84

Gene sequence:

>1275_bases
ATGACACTCAATATCAAGAACCATCCGTGCTTCAACGACTCGTCGAGACACACCTACGGAAGAATACACCTGCCGGTCGC
ACCGAAGTGCAACATCCAGTGCAACTACTGCAACCGGAAGTTCGACTGCATGAACGAAAACCGACCCGGCATCACCAGCA
AGGTGCTTTCGCCCAGGCAGGCGCTCTACTACCTCGACAACGCCCTGAAACTCTCGCCGAATATCTCGGTGGTGGGCATC
GCAGGCCCTGGCGACCCGTTCGCCAACCCGGAGGAGACGATGGAGACCCTGCGGCTCGTGCGTGAAAAGTACCCGGAGAT
GCTGCTCTGCGTCGCCACCAATGGCCTCGACATGCTGCCCTATATCGAGGAGCTTGCCGAGTTGCAGGTCAGCCACGTGA
CGCTGACAATCAACGCCATCGATCCGGAGATCGGTCAGGAGATCTACGCCTGGGTGCGCTACCAGAAGAAAATGTACCGC
GATCGCCAGGCCGCCGAGCTTTTGCTCGAAAACCAGCTCGCCGCTCTGCAGAAGCTCAAGAGGTATGGCGTAACGGCCAA
GGTCAACTCGATCATCATCCCCGGCGTCAACGACCAGCACGTGATTGAGGTGGCCCGTCAGGTCGCCTCGATGGGTGCGG
ACATTCTGAACGCGCTGCCTTACTACAACACGACGGAAACCGTGTTCGAGAACATTCCCGAACCTGATCCGATGATGGTC
AGGAAGATTCAGGAGGAGGCCGGAAAGCTCCTGCCGCAGATGAAGCACTGCGCCCGCTGCCGCGCCGACGCGGTCGGCAT
CATCGGCGAAATCAACAGCGACGAAATGATGGCGAAGCTCGCCGAAGCAGCGCTGATGCCGAAAAATCCCGACGAGCACC
GCCCCTACATCGCCGTGGCGAGCCTCGAAGGCGTGCTCATCAACCAGCATCTCGGCGAAGCCGACCGCTTTCTGGTCTAC
GCGCTCGACGAGGAGAAGAAAAGCTGCACACTGGTCGATTCACGCCAGGCCCCGCCTCCCGGCGGAGGCAAGTTGCGCTG
GGAAGCGCTCGCCGCGAAGCTCTCCGACTGCCGCGCTGTCTTGGTCAACAGCGCTGGCGATTCGCCGCAATCGGTTCTCA
AAGCCAGTGGCATCGACGTCATGTCGATCGAAGGGGTGATCGAAGAGGCCGTGTATGGCGTTTTCACCGGCCAGAACCTG
AAACACCTGATGAAAAGCAGCCAGATCCACGCATGCAAAACAAGCTGCGGCGGCGACGGCAACGGCTGCGACTAA

Upstream 100 bases:

>100_bases
CCGCATCGTCAACACCGTCATCGAAGAACGCCAGAAATCGTCGCCGATCGGCTACACCTATATGTAATGAAGAATCAATC
ATGACCTGAAGGGAGGCATC

Downstream 100 bases:

>100_bases
GTGATGATGAACTGTAAGGGCGATTCGCGAACCGCCCTTACTCAAACGATTCTTTGACCTATTAGCTTTACACAACCACC
ATCAAACAACTATCATGGAC

Product: NifB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTLNIKNHPCFNDSSRHTYGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGITSKVLSPRQALYYLDNALKLSPNISVVGI
AGPGDPFANPEETMETLRLVREKYPEMLLCVATNGLDMLPYIEELAELQVSHVTLTINAIDPEIGQEIYAWVRYQKKMYR
DRQAAELLLENQLAALQKLKRYGVTAKVNSIIIPGVNDQHVIEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
RKIQEEAGKLLPQMKHCARCRADAVGIIGEINSDEMMAKLAEAALMPKNPDEHRPYIAVASLEGVLINQHLGEADRFLVY
ALDEEKKSCTLVDSRQAPPPGGGKLRWEALAAKLSDCRAVLVNSAGDSPQSVLKASGIDVMSIEGVIEEAVYGVFTGQNL
KHLMKSSQIHACKTSCGGDGNGCD

Sequences:

>Translated_424_residues
MTLNIKNHPCFNDSSRHTYGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGITSKVLSPRQALYYLDNALKLSPNISVVGI
AGPGDPFANPEETMETLRLVREKYPEMLLCVATNGLDMLPYIEELAELQVSHVTLTINAIDPEIGQEIYAWVRYQKKMYR
DRQAAELLLENQLAALQKLKRYGVTAKVNSIIIPGVNDQHVIEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
RKIQEEAGKLLPQMKHCARCRADAVGIIGEINSDEMMAKLAEAALMPKNPDEHRPYIAVASLEGVLINQHLGEADRFLVY
ALDEEKKSCTLVDSRQAPPPGGGKLRWEALAAKLSDCRAVLVNSAGDSPQSVLKASGIDVMSIEGVIEEAVYGVFTGQNL
KHLMKSSQIHACKTSCGGDGNGCD
>Mature_423_residues
TLNIKNHPCFNDSSRHTYGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGITSKVLSPRQALYYLDNALKLSPNISVVGIA
GPGDPFANPEETMETLRLVREKYPEMLLCVATNGLDMLPYIEELAELQVSHVTLTINAIDPEIGQEIYAWVRYQKKMYRD
RQAAELLLENQLAALQKLKRYGVTAKVNSIIIPGVNDQHVIEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMVR
KIQEEAGKLLPQMKHCARCRADAVGIIGEINSDEMMAKLAEAALMPKNPDEHRPYIAVASLEGVLINQHLGEADRFLVYA
LDEEKKSCTLVDSRQAPPPGGGKLRWEALAAKLSDCRAVLVNSAGDSPQSVLKASGIDVMSIEGVIEEAVYGVFTGQNLK
HLMKSSQIHACKTSCGGDGNGCD

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 46794; Mature: 46663

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNIKNHPCFNDSSRHTYGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGITSKVLSPRQ
CEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCHHH
ALYYLDNALKLSPNISVVGIAGPGDPFANPEETMETLRLVREKYPEMLLCVATNGLDMLP
HHHHHCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
YIEELAELQVSHVTLTINAIDPEIGQEIYAWVRYQKKMYRDRQAAELLLENQLAALQKLK
HHHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGVTAKVNSIIIPGVNDQHVIEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
HCCCEEEECEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCHHHH
RKIQEEAGKLLPQMKHCARCRADAVGIIGEINSDEMMAKLAEAALMPKNPDEHRPYIAVA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
SLEGVLINQHLGEADRFLVYALDEEKKSCTLVDSRQAPPPGGGKLRWEALAAKLSDCRAV
ECCCEEEHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHEE
LVNSAGDSPQSVLKASGIDVMSIEGVIEEAVYGVFTGQNLKHLMKSSQIHACKTSCGGDG
EEECCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCCC
NGCD
CCCC
>Mature Secondary Structure 
TLNIKNHPCFNDSSRHTYGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGITSKVLSPRQ
EECCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCHHH
ALYYLDNALKLSPNISVVGIAGPGDPFANPEETMETLRLVREKYPEMLLCVATNGLDMLP
HHHHHCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
YIEELAELQVSHVTLTINAIDPEIGQEIYAWVRYQKKMYRDRQAAELLLENQLAALQKLK
HHHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGVTAKVNSIIIPGVNDQHVIEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
HCCCEEEECEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCHHHH
RKIQEEAGKLLPQMKHCARCRADAVGIIGEINSDEMMAKLAEAALMPKNPDEHRPYIAVA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
SLEGVLINQHLGEADRFLVYALDEEKKSCTLVDSRQAPPPGGGKLRWEALAAKLSDCRAV
ECCCEEEHHHCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHEE
LVNSAGDSPQSVLKASGIDVMSIEGVIEEAVYGVFTGQNLKHLMKSSQIHACKTSCGGDG
EEECCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCCC
NGCD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]