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The map label for this gene is nifE [H]

Identifier: 21674357

GI number: 21674357

Start: 1440819

End: 1442186

Strand: Direct

Name: nifE [H]

Synonym: CT1538

Alternate gene names: 21674357

Gene position: 1440819-1442186 (Clockwise)

Preceding gene: 21674356

Following gene: 21674358

Centisome position: 66.86

GC content: 57.68

Gene sequence:

>1368_bases
ATGGATACGCAGAAAATCAGCCTGCTCGAGGGCAGGGAAAAACAGGTCTATGAAAAGACCGCGGGTGGCGTAGAAGTGGA
CATCGCCTGCGACAAGACCAGCCTCTCCGGCTCGGTCAGCCAGCGTGCCTGTGTGTTCTGCGGTTCACGTGTCGTGCTCT
ATCCGGTAGCCGACGCCATTCACATCGTACACGGCCCGATCGGTTGCGCCGCCTACACCTGGGACATCCGGGGAGCGGTT
TCGTCGGGACCTGAACTGCACCGGCTCAGCTTTTCGACCGACCTCCAGGAGATGGACGTCATCTACGGCGGCGAAAAGAA
GCTCTACAAATCACTGATCGAACTGATCGACCAGTACCAGCCCAACGCGGCATTTATCTATTCGACCTGCATCATCGGCC
TGATTGGGGACGACATCGATGCGGTTTGCAAAAAAGTGGCCAAAGAGAAAGGAATCCCGGTCTTGCCGGTGCATTCCGAA
GGTTTCAAGGGCACCAAGAAGGATGGCTACAAAGCCGCCTGCATGGCCCTCATGAAGCTGATCGGCCAGGGATCGACCGA
GGGGATCAGCAAATACAGCATCAACATTCTCGGCGAATTCAACCTCGCCGGGGAAGCCTGGATCATCCGGGAATATTATG
AAAAGATGGGCATCGAGGTGGTCGCCACCATGACCGGCGACGGACGCATCGACGACATCCGCCGCTCGCACGGCGCGTCG
CTCAACGTGGTGCAGTGCTCCGGCTCGATGACCACCCTCGCCAAGGAGATGGAAGAGAAGTACGGCATCCCCTACATCCG
CGTCTCCTACTTCGGCTTCGAGGATATGTCCAAATCGCTCTACGACGTAGCCCAGCACTTCCCCGAAAGGCCCGACATTA
TGGAGAAAGCCAAGGAGATCGTGCGCGACGAAATCAGGAAATACTACCCCGAGATGCAGAAGTTCAAGGCCGCGTTGGCC
GGCAAGAAAGCTGCAATCTATGTCGGCGGAGCCTTCAAGACCTTCTCGCTCATCAAGGCGCTGCGCACCATCGGCATGTC
GGTCGTGCTCGCCGGCTCGCAGACCGGCAACAAGCAGGATTACAAGAACCTGAAAGAGATGTGCGACGAAGGCACGGTGA
TCGTGGACGACTCCAACCCGGTCGAACTCTCCAAGTTCGTGCTTGAAAAGGAGGCCGACCTTTTGATCGGCGGCGTCAAG
GAGCGCCCGATCGCCTTCAAGCTCGGCGTCGGCTTCTGCGACCACAACCACGAGCGCAAGATTCCGCTCGCCGGATTCAT
CGGCATGTACTATTTCGCCAAGGAGGTTTACGAATCGGTGATGAGCCCCGTGTGGCAGTTCGCCCCGAGAAAAGGAGGTG
CAAAATGA

Upstream 100 bases:

>100_bases
AACCGCTCACGACGACAACGTATCAGACAAGTTGACTGAAAGCTTGACAACCGTCCGCCTTCGATAACGGAAGGCGGGCG
ACAATGAAGGGGAAAAAGTA

Downstream 100 bases:

>100_bases
CGACCAACGCTTCGGCCATGACGGCCAAAACGGCCACGCAGAACGCCTGCAAGCTCTGCACTCCGCTCGGCGCGTGCCTG
GCTTTCAGGGGCATCGAATC

Product: nitrogenase iron-molybdenum cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MDTQKISLLEGREKQVYEKTAGGVEVDIACDKTSLSGSVSQRACVFCGSRVVLYPVADAIHIVHGPIGCAAYTWDIRGAV
SSGPELHRLSFSTDLQEMDVIYGGEKKLYKSLIELIDQYQPNAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPVLPVHSE
GFKGTKKDGYKAACMALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIEVVATMTGDGRIDDIRRSHGAS
LNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEIVRDEIRKYYPEMQKFKAALA
GKKAAIYVGGAFKTFSLIKALRTIGMSVVLAGSQTGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFVLEKEADLLIGGVK
ERPIAFKLGVGFCDHNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGAK

Sequences:

>Translated_455_residues
MDTQKISLLEGREKQVYEKTAGGVEVDIACDKTSLSGSVSQRACVFCGSRVVLYPVADAIHIVHGPIGCAAYTWDIRGAV
SSGPELHRLSFSTDLQEMDVIYGGEKKLYKSLIELIDQYQPNAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPVLPVHSE
GFKGTKKDGYKAACMALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIEVVATMTGDGRIDDIRRSHGAS
LNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEIVRDEIRKYYPEMQKFKAALA
GKKAAIYVGGAFKTFSLIKALRTIGMSVVLAGSQTGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFVLEKEADLLIGGVK
ERPIAFKLGVGFCDHNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGAK
>Mature_455_residues
MDTQKISLLEGREKQVYEKTAGGVEVDIACDKTSLSGSVSQRACVFCGSRVVLYPVADAIHIVHGPIGCAAYTWDIRGAV
SSGPELHRLSFSTDLQEMDVIYGGEKKLYKSLIELIDQYQPNAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPVLPVHSE
GFKGTKKDGYKAACMALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIEVVATMTGDGRIDDIRRSHGAS
LNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEIVRDEIRKYYPEMQKFKAALA
GKKAAIYVGGAFKTFSLIKALRTIGMSVVLAGSQTGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFVLEKEADLLIGGVK
ERPIAFKLGVGFCDHNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGAK

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 50230; Mature: 50230

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQKISLLEGREKQVYEKTAGGVEVDIACDKTSLSGSVSQRACVFCGSRVVLYPVADAI
CCCCHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEHHHHH
HIVHGPIGCAAYTWDIRGAVSSGPELHRLSFSTDLQEMDVIYGGEKKLYKSLIELIDQYQ
HHHCCCCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHEECCHHHHHHHHHHHHHHCC
PNAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPVLPVHSEGFKGTKKDGYKAACMALMKL
CCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
IGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIEVVATMTGDGRIDDIRRSHGAS
HCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCE
LNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEI
EEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VRDEIRKYYPEMQKFKAALAGKKAAIYVGGAFKTFSLIKALRTIGMSVVLAGSQTGNKQD
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
YKNLKEMCDEGTVIVDDSNPVELSKFVLEKEADLLIGGVKERPIAFKLGVGFCDHNHERK
HHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEECCCCCCEEEEECCEEECCCCCCC
IPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGAK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDTQKISLLEGREKQVYEKTAGGVEVDIACDKTSLSGSVSQRACVFCGSRVVLYPVADAI
CCCCHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEHHHHH
HIVHGPIGCAAYTWDIRGAVSSGPELHRLSFSTDLQEMDVIYGGEKKLYKSLIELIDQYQ
HHHCCCCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHEECCHHHHHHHHHHHHHHCC
PNAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPVLPVHSEGFKGTKKDGYKAACMALMKL
CCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
IGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIEVVATMTGDGRIDDIRRSHGAS
HCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCE
LNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEI
EEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VRDEIRKYYPEMQKFKAALAGKKAAIYVGGAFKTFSLIKALRTIGMSVVLAGSQTGNKQD
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
YKNLKEMCDEGTVIVDDSNPVELSKFVLEKEADLLIGGVKERPIAFKLGVGFCDHNHERK
HHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEECCCCCCEEEEECCEEECCCCCCC
IPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGAK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2726466 [H]