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The map label for this gene is nifK [H]

Identifier: 21674356

GI number: 21674356

Start: 1439262

End: 1440641

Strand: Direct

Name: nifK [H]

Synonym: CT1537

Alternate gene names: 21674356

Gene position: 1439262-1440641 (Clockwise)

Preceding gene: 21674355

Following gene: 21674357

Centisome position: 66.79

GC content: 59.71

Gene sequence:

>1380_bases
ATGTTATTACGTCATACCACAAAAGAAGTCAAAGAGCGCGAGGGGCTGACGATCAATCCGGCGAAAACCTGCCAGCCGAT
CGGCGCCATGTATGCCGCCCTCGGCATACACGGCTGCCTGCCCCACAGCCACGGCTCACAGGGCTGCTGCGCCTACCACC
GCAGCACCTTGACCCGCCACTACAAGGAGCCGGTGATGGCCGCCACCAGCTCGTTCACCGAAGGCGCTTCGGTGTTCGGC
GGCCAGGCCAACCTGCTTTCGGCCATCGAGACCATCTTTACGGTTTACGATCCGGAAGTGATCGCGGTGCACTCGACCTG
CCTGTCCGAAACCATCGGAGACGACTTGCAGCAGATCACCAAGAAGGCCAGCGATGACGGCAAGATTCCTGAAGGCAAGT
ACGTCATCTACGCCAGCACCCCGAGCTACGTGGGTTCGCACATCACCGGTTACGCCAACATGGTGACCAGCATGACCGAG
CAGTTCGCTGTCTCGACTGGCGAGAAGAAAGACCAGGTCAACGTCATCGCGGGCTGGATGGAACCGTCTGACATGCGCGA
AATCAAGTCGCTGGCCTCGCGCCTTGGTGTCAAGATCGTGCTCTTCCCAGACACCTCCGACGTGCTCGACGCGCCGCAGA
CCGGCAAGCACGAGTTCTATCCGAAAGGCGGCATCACCATCAATGAGCTGAAAAGCGCCGGTGACAGCAAATGCTCGCTC
GCGGTCGGCTGCATCAGCGCCGAACCGGCAGCAATCGCACTCGAAAAGAAGTGCAAGGTGCCCTTCGAAACCGTTGACAT
GCCGATCGGCCTGTCGGCAACCGACCGTTTCATCATGGCTCTCAGCAAAGCTGGCAGCGTGAAGGTACCGGATGAAATCA
CTGCCGAGCGTGGCCGTCTGGTAGACGTCATGGTCGACATGGAGCAGTACTTCTACGGCAAGAAGGTGGCGCTGTTCGGC
GACCCCGACCAGCTCATCCCGCTCACCGAGTTCCTGCTCGATCTGGGCATGATACCGGCACATATCGTCAGCGGCACGCC
GGGCCTGAGGTTCGAAAAACGCATGAAGGAGATCCTCGAACGGGCTCCGGGCGCGAACTTCCGCAACGGCCCGCAGGCAG
ACATGTTCCTGATGCACCAGTGGATCAAGAACGAGCCGGTTGACCTGCTCATCGGCAACACCTACGGCAAGTACATCGCA
CGTGACGAAGATATCCCGTTCGTGCGCTTCGGCTTCCCGATTCTCGACCGTATCGGCCACAGCTACTTCCCGAACGTCGG
GTACAGCGGCTCGCTCAGGCTGGTCGAGAAAATCCTCGGCGTGCTCATGGATCGTCAGGATCGCACGTCGCTGGAAGAGA
AGTTCGAGCTGGTTATGTGA

Upstream 100 bases:

>100_bases
TGGAAGATGATCAAGGCCCCCTGGGAGAAAAGTGAACCGGAGTCGCTGGAAGCCAGCTACGTCGCATCATAAGATGTAAA
ATATTAAAGGAGAATAATCG

Downstream 100 bases:

>100_bases
CGGCCTGCCGGAGCCGGTTCGCGATCCAGAGGCTCGCCCGGACGGTGAAATCCAGAACACTGTCCGGCTTGTCCGCGAAC
CGCTCACGACGACAACGTAT

Product: nitrogenase molybdenum-iron protein, beta subunit

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCAYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLSAIETIFTVYDPEVIAVHSTCLSETIGDDLQQITKKASDDGKIPEGKYVIYASTPSYVGSHITGYANMVTSMTE
QFAVSTGEKKDQVNVIAGWMEPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITINELKSAGDSKCSL
AVGCISAEPAAIALEKKCKVPFETVDMPIGLSATDRFIMALSKAGSVKVPDEITAERGRLVDVMVDMEQYFYGKKVALFG
DPDQLIPLTEFLLDLGMIPAHIVSGTPGLRFEKRMKEILERAPGANFRNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFVRFGFPILDRIGHSYFPNVGYSGSLRLVEKILGVLMDRQDRTSLEEKFELVM

Sequences:

>Translated_459_residues
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCAYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLSAIETIFTVYDPEVIAVHSTCLSETIGDDLQQITKKASDDGKIPEGKYVIYASTPSYVGSHITGYANMVTSMTE
QFAVSTGEKKDQVNVIAGWMEPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITINELKSAGDSKCSL
AVGCISAEPAAIALEKKCKVPFETVDMPIGLSATDRFIMALSKAGSVKVPDEITAERGRLVDVMVDMEQYFYGKKVALFG
DPDQLIPLTEFLLDLGMIPAHIVSGTPGLRFEKRMKEILERAPGANFRNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFVRFGFPILDRIGHSYFPNVGYSGSLRLVEKILGVLMDRQDRTSLEEKFELVM
>Mature_459_residues
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCAYHRSTLTRHYKEPVMAATSSFTEGASVFG
GQANLLSAIETIFTVYDPEVIAVHSTCLSETIGDDLQQITKKASDDGKIPEGKYVIYASTPSYVGSHITGYANMVTSMTE
QFAVSTGEKKDQVNVIAGWMEPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITINELKSAGDSKCSL
AVGCISAEPAAIALEKKCKVPFETVDMPIGLSATDRFIMALSKAGSVKVPDEITAERGRLVDVMVDMEQYFYGKKVALFG
DPDQLIPLTEFLLDLGMIPAHIVSGTPGLRFEKRMKEILERAPGANFRNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIA
RDEDIPFVRFGFPILDRIGHSYFPNVGYSGSLRLVEKILGVLMDRQDRTSLEEKFELVM

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50417; Mature: 50417

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCAYHRSTLTRH
CCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YKEPVMAATSSFTEGASVFGGQANLLSAIETIFTVYDPEVIAVHSTCLSETIGDDLQQIT
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
KKASDDGKIPEGKYVIYASTPSYVGSHITGYANMVTSMTEQFAVSTGEKKDQVNVIAGWM
HHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
EPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITINELKSAGDSKCSL
CCHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEE
AVGCISAEPAAIALEKKCKVPFETVDMPIGLSATDRFIMALSKAGSVKVPDEITAERGRL
EEEEECCCCCEEEEHHHCCCCHHHCCCCCCCCHHHHHHHHHHCCCCEECCHHHHHCCCCE
VDVMVDMEQYFYGKKVALFGDPDQLIPLTEFLLDLGMIPAHIVSGTPGLRFEKRMKEILE
EEEEEEHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHH
RAPGANFRNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIARDEDIPFVRFGFPILDRIGH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCEEEECCHHHHHCCH
SYFPNVGYSGSLRLVEKILGVLMDRQDRTSLEEKFELVM
HHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLLRHTTKEVKEREGLTINPAKTCQPIGAMYAALGIHGCLPHSHGSQGCCAYHRSTLTRH
CCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
YKEPVMAATSSFTEGASVFGGQANLLSAIETIFTVYDPEVIAVHSTCLSETIGDDLQQIT
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
KKASDDGKIPEGKYVIYASTPSYVGSHITGYANMVTSMTEQFAVSTGEKKDQVNVIAGWM
HHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
EPSDMREIKSLASRLGVKIVLFPDTSDVLDAPQTGKHEFYPKGGITINELKSAGDSKCSL
CCHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEE
AVGCISAEPAAIALEKKCKVPFETVDMPIGLSATDRFIMALSKAGSVKVPDEITAERGRL
EEEEECCCCCEEEEHHHCCCCHHHCCCCCCCCHHHHHHHHHHCCCCEECCHHHHHCCCCE
VDVMVDMEQYFYGKKVALFGDPDQLIPLTEFLLDLGMIPAHIVSGTPGLRFEKRMKEILE
EEEEEEHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHH
RAPGANFRNGPQADMFLMHQWIKNEPVDLLIGNTYGKYIARDEDIPFVRFGFPILDRIGH
HCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHCCCCCCCEEEECCHHHHHCCH
SYFPNVGYSGSLRLVEKILGVLMDRQDRTSLEEKFELVM
HHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840948; 3473447; 8393705 [H]