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The map label for this gene is nifA [H]

Identifier: 21674349

GI number: 21674349

Start: 1432952

End: 1434574

Strand: Direct

Name: nifA [H]

Synonym: CT1529

Alternate gene names: 21674349

Gene position: 1432952-1434574 (Clockwise)

Preceding gene: 21674348

Following gene: 21674350

Centisome position: 66.5

GC content: 57.55

Gene sequence:

>1623_bases
ATGCTCATCGCTCAGGAACAGGAACAGAGCAGCATCAGTCTACTCGCCGAGGTCAGCAGGGCCGTCAACAATGAGGAGGA
CATCAGCAAGGTGCTCCGGCTGGTGCTTTTCATCTTGTCAGAACATATGAACATGCTCAGAGGCATGGTCACCATTCTGA
ACCGCACTACCGGCGAGATGGTCATCAACGAATCGTTCGGCCTGACCGACGAGCAGCGTCAGCTTGCCCGCTACAAGGTT
GGCGAAGGCATCATCGGCCAGGTGGTCAAAACGGGACGCCCCTCCGTCGTGCCCCGCATCAATGAAGAGCCGCTTTTTCT
CGACCGCACCCAGTCCAGAGCCGAGGAAAACAAGGAGGAGTTGTGCTTCATCTGCGTGCCCATCAAGGTGGGACGCGAGG
TTATCGGCACACTGAGCGCTGACCGGCGAATTTCCGTTCCCGCTGAAAATGGCGACAAGAAGCTCTGGCGCAAAAGTGAC
CGGATCGATGTTCTGCAGTACTATGTCGATCTGCTCTCGATCATTGCGGCCATGATCGCCCAGGCGGTTCGCATCAAGCA
GCTCGATGACGAAAAGAGCATCGATGGCGCACACGAACGAACCGGTACCCCTGCACTGGGCTTCCAGATCATCGATAGAG
AAATCGAAGAAGAGTTGCCCGAAACGGAACGGCCCGCCAACATCATCGGAAACGCCAAGCCGATGATGTCGCTCTTCAAG
ATGATCGACAAAATCTCGAAAACCAGCGCTACGGCCCTGATTCTCGGCGAAAGCGGCGTCGGCAAGGAGCTGGTGGCCAA
CGCCATCCACTTCAAAAGCATGAGGAATGGCAAGCCGTTCATCAAGTTCAACTGCGCGGCGCTGCCGGAAAGCATTGTCG
AAAGTGAGCTGTTCGGCCATGAAAAAGGAGCCTTCACCGGCGCCTCGGCACAGCGGCGCGGGCGCTTCGAGCTTGCCGAC
GGCGGCACGCTTTTTCTCGACGAGGTCGGCGAGCTGAGCCTGCCAACGCAGGCCAAGTTGCTGCGAATCATCCAGGAGAA
GGAGTTCGAGCGGGTCGGCGGATCGAAAACCATCAAAACCGACGTGCGCATCATCGCAGCCACCAACCGGAACCTCGAGG
AACAGATCCAGAAAGGCAACTTCCGCGAGGATCTCTACTACCGACTCAACATCTTCCCGATCACCGTGCCGCCGCTTCGC
GAGCGCAAGACGGATATTTTGCTGCTGGCGGACTATTTCGTGGAGCAGTTCAACAAGACCAACCACAAGGGTGTGCGGCG
GATTTCGACCACGGCCATCGACATGCTGATGCGCTACCACTGGCCCGGCAACGTGCGCGAGTTGCAGAACTGCATCGAAC
GCGCGGTGATTCTGAGCGAGGACAACGTCATCCACGGCTACCACCTGCCGCCGTCGCTCCAGACCGCCGAGTCAAGCGGC
ACCCCCTACACCGGCGACTTGCAGCAGAAACTCGACGCCATCGAGAAGGAGATGATCATCGAAGCGCTCAAACGCACCAA
AGGCAACATGACCAAAGCCGCCATCCAACTCGGCCTGTCGGACAGGATCATGGGATTAAGGATGAAGAAATTCAATATCG
ACTACCGGAAATTCCGGGCGTGA

Upstream 100 bases:

>100_bases
GTAGTCAGTCCGACCGCCTCAAACGGGCGCTCCGGACCGACGAGATCGACGCGCTCCGTGAACAAAACTCTGTGAAACAC
GCTTAACTCGAATAGTTTTC

Downstream 100 bases:

>100_bases
GAGAAAAAAAAGGCTCCTCGCTGCTTCGAGTGGGTAACGTGTGAGAAGCGTATCTTTTGGTTTGCACATTTTACCACTTC
GCCTAAAAAAGACCTCAACC

Product: nitrogen fixation specific regulatory protein NifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLIAQEQEQSSISLLAEVSRAVNNEEDISKVLRLVLFILSEHMNMLRGMVTILNRTTGEMVINESFGLTDEQRQLARYKV
GEGIIGQVVKTGRPSVVPRINEEPLFLDRTQSRAEENKEELCFICVPIKVGREVIGTLSADRRISVPAENGDKKLWRKSD
RIDVLQYYVDLLSIIAAMIAQAVRIKQLDDEKSIDGAHERTGTPALGFQIIDREIEEELPETERPANIIGNAKPMMSLFK
MIDKISKTSATALILGESGVGKELVANAIHFKSMRNGKPFIKFNCAALPESIVESELFGHEKGAFTGASAQRRGRFELAD
GGTLFLDEVGELSLPTQAKLLRIIQEKEFERVGGSKTIKTDVRIIAATNRNLEEQIQKGNFREDLYYRLNIFPITVPPLR
ERKTDILLLADYFVEQFNKTNHKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSG
TPYTGDLQQKLDAIEKEMIIEALKRTKGNMTKAAIQLGLSDRIMGLRMKKFNIDYRKFRA

Sequences:

>Translated_540_residues
MLIAQEQEQSSISLLAEVSRAVNNEEDISKVLRLVLFILSEHMNMLRGMVTILNRTTGEMVINESFGLTDEQRQLARYKV
GEGIIGQVVKTGRPSVVPRINEEPLFLDRTQSRAEENKEELCFICVPIKVGREVIGTLSADRRISVPAENGDKKLWRKSD
RIDVLQYYVDLLSIIAAMIAQAVRIKQLDDEKSIDGAHERTGTPALGFQIIDREIEEELPETERPANIIGNAKPMMSLFK
MIDKISKTSATALILGESGVGKELVANAIHFKSMRNGKPFIKFNCAALPESIVESELFGHEKGAFTGASAQRRGRFELAD
GGTLFLDEVGELSLPTQAKLLRIIQEKEFERVGGSKTIKTDVRIIAATNRNLEEQIQKGNFREDLYYRLNIFPITVPPLR
ERKTDILLLADYFVEQFNKTNHKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSG
TPYTGDLQQKLDAIEKEMIIEALKRTKGNMTKAAIQLGLSDRIMGLRMKKFNIDYRKFRA
>Mature_540_residues
MLIAQEQEQSSISLLAEVSRAVNNEEDISKVLRLVLFILSEHMNMLRGMVTILNRTTGEMVINESFGLTDEQRQLARYKV
GEGIIGQVVKTGRPSVVPRINEEPLFLDRTQSRAEENKEELCFICVPIKVGREVIGTLSADRRISVPAENGDKKLWRKSD
RIDVLQYYVDLLSIIAAMIAQAVRIKQLDDEKSIDGAHERTGTPALGFQIIDREIEEELPETERPANIIGNAKPMMSLFK
MIDKISKTSATALILGESGVGKELVANAIHFKSMRNGKPFIKFNCAALPESIVESELFGHEKGAFTGASAQRRGRFELAD
GGTLFLDEVGELSLPTQAKLLRIIQEKEFERVGGSKTIKTDVRIIAATNRNLEEQIQKGNFREDLYYRLNIFPITVPPLR
ERKTDILLLADYFVEQFNKTNHKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSG
TPYTGDLQQKLDAIEKEMIIEALKRTKGNMTKAAIQLGLSDRIMGLRMKKFNIDYRKFRA

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=328, Percent_Identity=47.8658536585366, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI1790437, Length=320, Percent_Identity=48.125, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082117, Length=558, Percent_Identity=33.6917562724014, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI1789087, Length=344, Percent_Identity=46.5116279069767, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI87082152, Length=306, Percent_Identity=46.7320261437908, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=40.1812688821752, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1788905, Length=313, Percent_Identity=43.4504792332268, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=42.2018348623853, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=39.4984326018809, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=41.1585365853659, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=38.3177570093458, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789828, Length=237, Percent_Identity=38.3966244725738, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI87081858, Length=298, Percent_Identity=31.8791946308725, Blast_Score=148, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60957; Mature: 60957

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAQEQEQSSISLLAEVSRAVNNEEDISKVLRLVLFILSEHMNMLRGMVTILNRTTGEM
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VINESFGLTDEQRQLARYKVGEGIIGQVVKTGRPSVVPRINEEPLFLDRTQSRAEENKEE
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHCCCCC
LCFICVPIKVGREVIGTLSADRRISVPAENGDKKLWRKSDRIDVLQYYVDLLSIIAAMIA
EEEEEEEHHHHHHHHHHCCCCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAVRIKQLDDEKSIDGAHERTGTPALGFQIIDREIEEELPETERPANIIGNAKPMMSLFK
HHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHH
MIDKISKTSATALILGESGVGKELVANAIHFKSMRNGKPFIKFNCAALPESIVESELFGH
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCC
EKGAFTGASAQRRGRFELADGGTLFLDEVGELSLPTQAKLLRIIQEKEFERVGGSKTIKT
CCCCCCCCCHHHCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVRIIAATNRNLEEQIQKGNFREDLYYRLNIFPITVPPLRERKTDILLLADYFVEQFNKT
CEEEEEECCCCHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHCCC
NHKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSG
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCC
TPYTGDLQQKLDAIEKEMIIEALKRTKGNMTKAAIQLGLSDRIMGLRMKKFNIDYRKFRA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHEEHHHCCCCHHHHCC
>Mature Secondary Structure
MLIAQEQEQSSISLLAEVSRAVNNEEDISKVLRLVLFILSEHMNMLRGMVTILNRTTGEM
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VINESFGLTDEQRQLARYKVGEGIIGQVVKTGRPSVVPRINEEPLFLDRTQSRAEENKEE
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEECHHHHHHCCCCC
LCFICVPIKVGREVIGTLSADRRISVPAENGDKKLWRKSDRIDVLQYYVDLLSIIAAMIA
EEEEEEEHHHHHHHHHHCCCCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAVRIKQLDDEKSIDGAHERTGTPALGFQIIDREIEEELPETERPANIIGNAKPMMSLFK
HHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHH
MIDKISKTSATALILGESGVGKELVANAIHFKSMRNGKPFIKFNCAALPESIVESELFGH
HHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCC
EKGAFTGASAQRRGRFELADGGTLFLDEVGELSLPTQAKLLRIIQEKEFERVGGSKTIKT
CCCCCCCCCHHHCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVRIIAATNRNLEEQIQKGNFREDLYYRLNIFPITVPPLRERKTDILLLADYFVEQFNKT
CEEEEEECCCCHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHCCC
NHKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPPSLQTAESSG
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCC
TPYTGDLQQKLDAIEKEMIIEALKRTKGNMTKAAIQLGLSDRIMGLRMKKFNIDYRKFRA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHEEHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]