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The map label for this gene is nifV [H]

Identifier: 21674348

GI number: 21674348

Start: 1431807

End: 1432937

Strand: Direct

Name: nifV [H]

Synonym: CT1528

Alternate gene names: 21674348

Gene position: 1431807-1432937 (Clockwise)

Preceding gene: 21674347

Following gene: 21674349

Centisome position: 66.44

GC content: 60.74

Gene sequence:

>1131_bases
GTGATCCGCAAACCGTGGATCATCGATACGACCCTCCGGGATGGAGAGCAGGCCCCCGGCGTGGTGTTCAGTCCGCACGA
AAAAAAGCGGATAGCAGCAATGCTTGCAGAAACCGGCGTCGATGAGATCGAAGTGGGCTACCCGGCCATCAGTGCGGCGG
AGCGGAAGGTGATTCGCGAGATCGTCGCCATGAAACTGCCGGTACGCCTGACTTCGTGGTCGCGGGCCAATATGGCGGAC
ATCGAGTTGGCGGCAGAATGCGGCACCGATGCGGTGCACATCAGTTTTCCGGCCTCCCGGCTCTATCTGGAGCTGATACA
CAAGAAGGATGACTGGATTCAGGAGCAGCTTCACGCGCTTGTTTCCAAAGCTCGCGAACGGTTCGATTTCGTCAGCGTCG
GCGGACAGGACGCCACGAGAAGTAGCACTGATTTTCTGCAACGCTTCATGCTCGATGCTGAAGCTGCCGGAGCCAAACGG
TTCAGGATCGCCGATACGGTAGGCATCGCCACGCCGGTGTCGGTGATGGCCCTCGGCGCAGCGTTGCGCCAAAGCTCGTC
GCTGCCGCTGGAGTTCCACGCGCACAACGATCTCGGCATGGCGACCGCCAACGCCTTCACGGCACTCAACGAGGGATTCG
AAGCGGTCAGCGTGTCGGTGACCGGTCTCGGAGAGCGTGCGGGCAACGCGGCCCTCGAAGAGCTGGCAATGGCGCTTGCG
CTGAATGGAGACTTCGACACGCATCTCGACACATCGATGCTCTCCCGGCTTTGCGACGCCGTGGCCACGGCTTCCGGACG
AGCCATTCAGGAGCAAAAACCGGTGGTCGGACGTTCAGCCTTCCAGCACGAATCGGGCATTCATTGCGCCGCGCTGTTGC
AGGATCCGCTCTCCTACCAGCCGTTCCTGCCGTCGCGGGTTGGCCGCAGCGATTTCGAGATAGTCATCGGCAAACACTCA
GGCACAGCGGCGATCATCGCCCATTTCAACCGGCGCGGAATCACGATTTCGAAAAAGGAGGCCCGCGAACTGCTCGATCT
GATCCGTAGTCAGTCCGACCGCCTCAAACGGGCGCTCCGGACCGACGAGATCGACGCGCTCCGTGAACAAAACTCTGTGA
AACACGCTTAA

Upstream 100 bases:

>100_bases
ATTTTTATGATTAGTTTTATATTTACCTAAATTAAAAGGATCAGTAAAGTCAATACGCAAAAAAACACCCGTGCCAACCC
GAACTGAAACCAGACCCGGA

Downstream 100 bases:

>100_bases
CTCGAATAGTTTTCATGCTCATCGCTCAGGAACAGGAACAGAGCAGCATCAGTCTACTCGCCGAGGTCAGCAGGGCCGTC
AACAATGAGGAGGACATCAG

Product: homocitrate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIRKPWIIDTTLRDGEQAPGVVFSPHEKKRIAAMLAETGVDEIEVGYPAISAAERKVIREIVAMKLPVRLTSWSRANMAD
IELAAECGTDAVHISFPASRLYLELIHKKDDWIQEQLHALVSKARERFDFVSVGGQDATRSSTDFLQRFMLDAEAAGAKR
FRIADTVGIATPVSVMALGAALRQSSSLPLEFHAHNDLGMATANAFTALNEGFEAVSVSVTGLGERAGNAALEELAMALA
LNGDFDTHLDTSMLSRLCDAVATASGRAIQEQKPVVGRSAFQHESGIHCAALLQDPLSYQPFLPSRVGRSDFEIVIGKHS
GTAAIIAHFNRRGITISKKEARELLDLIRSQSDRLKRALRTDEIDALREQNSVKHA

Sequences:

>Translated_376_residues
MIRKPWIIDTTLRDGEQAPGVVFSPHEKKRIAAMLAETGVDEIEVGYPAISAAERKVIREIVAMKLPVRLTSWSRANMAD
IELAAECGTDAVHISFPASRLYLELIHKKDDWIQEQLHALVSKARERFDFVSVGGQDATRSSTDFLQRFMLDAEAAGAKR
FRIADTVGIATPVSVMALGAALRQSSSLPLEFHAHNDLGMATANAFTALNEGFEAVSVSVTGLGERAGNAALEELAMALA
LNGDFDTHLDTSMLSRLCDAVATASGRAIQEQKPVVGRSAFQHESGIHCAALLQDPLSYQPFLPSRVGRSDFEIVIGKHS
GTAAIIAHFNRRGITISKKEARELLDLIRSQSDRLKRALRTDEIDALREQNSVKHA
>Mature_376_residues
MIRKPWIIDTTLRDGEQAPGVVFSPHEKKRIAAMLAETGVDEIEVGYPAISAAERKVIREIVAMKLPVRLTSWSRANMAD
IELAAECGTDAVHISFPASRLYLELIHKKDDWIQEQLHALVSKARERFDFVSVGGQDATRSSTDFLQRFMLDAEAAGAKR
FRIADTVGIATPVSVMALGAALRQSSSLPLEFHAHNDLGMATANAFTALNEGFEAVSVSVTGLGERAGNAALEELAMALA
LNGDFDTHLDTSMLSRLCDAVATASGRAIQEQKPVVGRSAFQHESGIHCAALLQDPLSYQPFLPSRVGRSDFEIVIGKHS
GTAAIIAHFNRRGITISKKEARELLDLIRSQSDRLKRALRTDEIDALREQNSVKHA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=375, Percent_Identity=29.8666666666667, Blast_Score=150, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=370, Percent_Identity=30.8108108108108, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320071, Length=375, Percent_Identity=30.1333333333333, Blast_Score=157, Evalue=3e-39,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 41033; Mature: 41033

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKPWIIDTTLRDGEQAPGVVFSPHEKKRIAAMLAETGVDEIEVGYPAISAAERKVIRE
CCCCCEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHH
IVAMKLPVRLTSWSRANMADIELAAECGTDAVHISFPASRLYLELIHKKDDWIQEQLHAL
HHHHHCCEEEECCCCCCCHHHEEHHHCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHH
VSKARERFDFVSVGGQDATRSSTDFLQRFMLDAEAAGAKRFRIADTVGIATPVSVMALGA
HHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHHHH
ALRQSSSLPLEFHAHNDLGMATANAFTALNEGFEAVSVSVTGLGERAGNAALEELAMALA
HHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
LNGDFDTHLDTSMLSRLCDAVATASGRAIQEQKPVVGRSAFQHESGIHCAALLQDPLSYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHCCCCCHHHHHHCCCCCC
PFLPSRVGRSDFEIVIGKHSGTAAIIAHFNRRGITISKKEARELLDLIRSQSDRLKRALR
CCCCHHCCCCCEEEEEECCCCCEEEEEEECCCCCEECHHHHHHHHHHHHCHHHHHHHHHH
TDEIDALREQNSVKHA
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIRKPWIIDTTLRDGEQAPGVVFSPHEKKRIAAMLAETGVDEIEVGYPAISAAERKVIRE
CCCCCEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHH
IVAMKLPVRLTSWSRANMADIELAAECGTDAVHISFPASRLYLELIHKKDDWIQEQLHAL
HHHHHCCEEEECCCCCCCHHHEEHHHCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHH
VSKARERFDFVSVGGQDATRSSTDFLQRFMLDAEAAGAKRFRIADTVGIATPVSVMALGA
HHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHHHH
ALRQSSSLPLEFHAHNDLGMATANAFTALNEGFEAVSVSVTGLGERAGNAALEELAMALA
HHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
LNGDFDTHLDTSMLSRLCDAVATASGRAIQEQKPVVGRSAFQHESGIHCAALLQDPLSYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHCCCCCHHHHHHCCCCCC
PFLPSRVGRSDFEIVIGKHSGTAAIIAHFNRRGITISKKEARELLDLIRSQSDRLKRALR
CCCCHHCCCCCEEEEEECCCCCEEEEEEECCCCCEECHHHHHHHHHHHHCHHHHHHHHHH
TDEIDALREQNSVKHA
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]