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The map label for this gene is 21674330

Identifier: 21674330

GI number: 21674330

Start: 1416960

End: 1417835

Strand: Reverse

Name: 21674330

Synonym: CT1510

Alternate gene names: NA

Gene position: 1417835-1416960 (Counterclockwise)

Preceding gene: 21674331

Following gene: 21674329

Centisome position: 65.79

GC content: 61.3

Gene sequence:

>876_bases
ATGAATACCGATCAGGTTCGCATAGCGCTCGTCCAGATGAGCTGCGTTGAGAATCCGCAGGAAAACCTCAGGAAAGCGCA
GGAGCGCATCCGCCAGGCTGCCGCGGGCGGGGCCAATATCGTCTGCCTTCAGGAGCTGTTCACCACGCTCTACTTCTGCC
AGACCGAGGAGTACGAGCCGTTTGGATACGCCGAGCCGATTCCCGGCCCGTCGACCGCTGCATTGCAGGAGCTGGCCGCC
GAACTTGGGGTGGTGATCGTGGCTTCGCTCTTTGAGATTCGCGCCAAGGGGGTGCACCACAACACGGCGGCGGTGATCGA
TGCTGACGGCAGCTACCTCGGCAAGTACCGCAAGATGCACATTCCCGACGATCCGGGCTTCTACGAGAAGTTCTACTTTG
TGCCCGGCGACCTCGGTTACAAGGTGTTTGATACGAAATTTGGTACGATCGGCGTCCTGATTTGCTGGGATCAGTGGTAC
CCCGAAGCCGCGCGACTTACGGCGCTGCGCGGAGCTGATATTCTCTTCTATCCCACGGCCATCGGCTGGGCCACCTCCGA
GACCTCGCAGGAGGTTCGCGCGAGCCAGCGGCAGGCGTGGAAAACCTCGCATCTCGGTCACGCCGTGGCAAACGGTGTGT
TTGTCGCCGCCGCCAATCGTGCGGGCACGGAGGGCGAACTCGAGTTCTGGGGCAACAGCTTCGTCTCCGATCCCTTCGGT
CAGGTGATTGCCGAGGCGGCCCACAACAGCGAGGAGATTCTCTACGCCGACTGCGACCTCTCGAAGATCGGCTTTTACCG
CTCGCACTGGCCCTTCATGCGCGACCGCAGGATCGACTCCTATGGCGACATCACGCGGCGCTGGATCGACGAATAA

Upstream 100 bases:

>100_bases
TGAATGCGGTGCTGGCTGGCACCGGCAAGGTGCTTACCGTGCACGGCTTACTCTTTTCGCTCGGGCTTGTCATTCCCAAT
ATCATTTCGATTTTTCGTCC

Downstream 100 bases:

>100_bases
ACCGTTATGATGATGAACCTGTTCGGCCAAATCATTCTTTTTACGCTTATCGGCACCTGGCTCATCAAACTTGCCGCCGA
TCTTTTGAACCTTCGTGCTG

Product: carbon-nitrogen hydrolase family protein

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNTDQVRIALVQMSCVENPQENLRKAQERIRQAAAGGANIVCLQELFTTLYFCQTEEYEPFGYAEPIPGPSTAALQELAA
ELGVVIVASLFEIRAKGVHHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFVPGDLGYKVFDTKFGTIGVLICWDQWY
PEAARLTALRGADILFYPTAIGWATSETSQEVRASQRQAWKTSHLGHAVANGVFVAAANRAGTEGELEFWGNSFVSDPFG
QVIAEAAHNSEEILYADCDLSKIGFYRSHWPFMRDRRIDSYGDITRRWIDE

Sequences:

>Translated_291_residues
MNTDQVRIALVQMSCVENPQENLRKAQERIRQAAAGGANIVCLQELFTTLYFCQTEEYEPFGYAEPIPGPSTAALQELAA
ELGVVIVASLFEIRAKGVHHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFVPGDLGYKVFDTKFGTIGVLICWDQWY
PEAARLTALRGADILFYPTAIGWATSETSQEVRASQRQAWKTSHLGHAVANGVFVAAANRAGTEGELEFWGNSFVSDPFG
QVIAEAAHNSEEILYADCDLSKIGFYRSHWPFMRDRRIDSYGDITRRWIDE
>Mature_291_residues
MNTDQVRIALVQMSCVENPQENLRKAQERIRQAAAGGANIVCLQELFTTLYFCQTEEYEPFGYAEPIPGPSTAALQELAA
ELGVVIVASLFEIRAKGVHHNTAAVIDADGSYLGKYRKMHIPDDPGFYEKFYFVPGDLGYKVFDTKFGTIGVLICWDQWY
PEAARLTALRGADILFYPTAIGWATSETSQEVRASQRQAWKTSHLGHAVANGVFVAAANRAGTEGELEFWGNSFVSDPFG
QVIAEAAHNSEEILYADCDLSKIGFYRSHWPFMRDRRIDSYGDITRRWIDE

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=282, Percent_Identity=35.8156028368794, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI7706509, Length=279, Percent_Identity=31.8996415770609, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI297632350, Length=288, Percent_Identity=28.4722222222222, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI297632348, Length=288, Percent_Identity=28.4722222222222, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI5031947, Length=288, Percent_Identity=28.4722222222222, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI297632346, Length=188, Percent_Identity=28.1914893617021, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17533173, Length=304, Percent_Identity=31.9078947368421, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17556280, Length=297, Percent_Identity=26.2626262626263, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=298, Percent_Identity=30.8724832214765, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322335, Length=313, Percent_Identity=23.961661341853, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21358471, Length=309, Percent_Identity=32.0388349514563, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21355835, Length=289, Percent_Identity=27.3356401384083, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17933642, Length=280, Percent_Identity=26.0714285714286, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32583; Mature: 32583

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDQVRIALVQMSCVENPQENLRKAQERIRQAAAGGANIVCLQELFTTLYFCQTEEYEP
CCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCC
FGYAEPIPGPSTAALQELAAELGVVIVASLFEIRAKGVHHNTAAVIDADGSYLGKYRKMH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCC
IPDDPGFYEKFYFVPGDLGYKVFDTKFGTIGVLICWDQWYPEAARLTALRGADILFYPTA
CCCCCCCCCEEEECCCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHCCCCCEEEECCC
IGWATSETSQEVRASQRQAWKTSHLGHAVANGVFVAAANRAGTEGELEFWGNSFVSDPFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCCCHHH
QVIAEAAHNSEEILYADCDLSKIGFYRSHWPFMRDRRIDSYGDITRRWIDE
HHHHHHHCCCCCEEEEECCCHHHCCHHHCCCCHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNTDQVRIALVQMSCVENPQENLRKAQERIRQAAAGGANIVCLQELFTTLYFCQTEEYEP
CCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCC
FGYAEPIPGPSTAALQELAAELGVVIVASLFEIRAKGVHHNTAAVIDADGSYLGKYRKMH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCC
IPDDPGFYEKFYFVPGDLGYKVFDTKFGTIGVLICWDQWYPEAARLTALRGADILFYPTA
CCCCCCCCCEEEECCCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHCCCCCEEEECCC
IGWATSETSQEVRASQRQAWKTSHLGHAVANGVFVAAANRAGTEGELEFWGNSFVSDPFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCCCHHH
QVIAEAAHNSEEILYADCDLSKIGFYRSHWPFMRDRRIDSYGDITRRWIDE
HHHHHHHCCCCCEEEEECCCHHHCCHHHCCCCHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]