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The map label for this gene is grpE

Identifier: 21674305

GI number: 21674305

Start: 1390557

End: 1391141

Strand: Reverse

Name: grpE

Synonym: CT1485

Alternate gene names: 21674305

Gene position: 1391141-1390557 (Counterclockwise)

Preceding gene: 21674306

Following gene: 21674304

Centisome position: 64.56

GC content: 59.32

Gene sequence:

>585_bases
ATGAGCAGAAAGCACCACAAGGAACAGGAAGAGATACAGGAGCAGGAGACGATCAGCGCTGGGGCGGCTGAAACGCCTGC
CGAAGAGACGGCGGCGATTCCTGCGGCGACCGAGGCTGACATGGATGCCGAAATCTCGGCTCGCGATGCCGAGATACAGA
AGCTGCGCGAAGAGGTGATGCGCCGCGCCGCCGAGTTCGAGAATTTCCGGAAGCAGAAAGAGCGCGAAGCCGCGCTGTCG
GGTACGCGGATGCTGGAGAACATCGTGCGCGAACTGTTGCCGCTGATCGATGACCTGAAGCGTCTCATGAGCCACATTCC
CGCCGAAATGCAGGCGATGGCCGAGGCCAAACCTTTTATCGAGGGCGTGGAACTGATCCACAAAAACTTCATGTCGCTGC
TTGAGCGCAAAGGGGTCAAGGAGATCGAGGCCAAAGGCAAGATGCTCGATGTCAACTTCCACGAAGCGATCACGCAGATC
GACGCGCCGGGCGCGGAGCCCGACACCATCGTCGAGGAGTACCAGACCGGCTACACGCTTGGAGACAGGGTGATCCGTCA
TGCCAAGGTGATCGTCGCGAAATAA

Upstream 100 bases:

>100_bases
ATGAATTACGAACACGCCGTGCGCGTAGTGAACTACATGGCGGGCTGCCTTTCAGAGGCGCTCTCCGGAAATAATTAATT
TTAAATATTTTTTCGTCATT

Downstream 100 bases:

>100_bases
CTATCAGGTTATCAGGTTCAGATATAAATAGTGAGAGTATAAACGATGAAGAGAGATTACTATGAAATCCTTGGCGTCGC
CCGTTCGGCGGACAAGGATG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSRKHHKEQEEIQEQETISAGAAETPAEETAAIPAATEADMDAEISARDAEIQKLREEVMRRAAEFENFRKQKEREAALS
GTRMLENIVRELLPLIDDLKRLMSHIPAEMQAMAEAKPFIEGVELIHKNFMSLLERKGVKEIEAKGKMLDVNFHEAITQI
DAPGAEPDTIVEEYQTGYTLGDRVIRHAKVIVAK

Sequences:

>Translated_194_residues
MSRKHHKEQEEIQEQETISAGAAETPAEETAAIPAATEADMDAEISARDAEIQKLREEVMRRAAEFENFRKQKEREAALS
GTRMLENIVRELLPLIDDLKRLMSHIPAEMQAMAEAKPFIEGVELIHKNFMSLLERKGVKEIEAKGKMLDVNFHEAITQI
DAPGAEPDTIVEEYQTGYTLGDRVIRHAKVIVAK
>Mature_193_residues
SRKHHKEQEEIQEQETISAGAAETPAEETAAIPAATEADMDAEISARDAEIQKLREEVMRRAAEFENFRKQKEREAALSG
TRMLENIVRELLPLIDDLKRLMSHIPAEMQAMAEAKPFIEGVELIHKNFMSLLERKGVKEIEAKGKMLDVNFHEAITQID
APGAEPDTIVEEYQTGYTLGDRVIRHAKVIVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=156, Percent_Identity=34.6153846153846, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=155, Percent_Identity=27.741935483871, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24653432, Length=155, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CHLTE (Q8KCD7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662370.1
- ProteinModelPortal:   Q8KCD7
- SMR:   Q8KCD7
- GeneID:   1006030
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1485
- NMPDR:   fig|194439.1.peg.1464
- TIGR:   CT1485
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   CLSK637785
- BioCyc:   CTEP194439:CT_1485-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21890; Mature: 21759

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKHHKEQEEIQEQETISAGAAETPAEETAAIPAATEADMDAEISARDAEIQKLREEVM
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RRAAEFENFRKQKEREAALSGTRMLENIVRELLPLIDDLKRLMSHIPAEMQAMAEAKPFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EGVELIHKNFMSLLERKGVKEIEAKGKMLDVNFHEAITQIDAPGAEPDTIVEEYQTGYTL
HHHHHHHHHHHHHHHHCCHHHHHHCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHCCCHH
GDRVIRHAKVIVAK
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SRKHHKEQEEIQEQETISAGAAETPAEETAAIPAATEADMDAEISARDAEIQKLREEVM
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RRAAEFENFRKQKEREAALSGTRMLENIVRELLPLIDDLKRLMSHIPAEMQAMAEAKPFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EGVELIHKNFMSLLERKGVKEIEAKGKMLDVNFHEAITQIDAPGAEPDTIVEEYQTGYTL
HHHHHHHHHHHHHHHHCCHHHHHHCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHCCCHH
GDRVIRHAKVIVAK
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901