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The map label for this gene is lepA [H]

Identifier: 21674272

GI number: 21674272

Start: 1362391

End: 1364208

Strand: Reverse

Name: lepA [H]

Synonym: CT1451

Alternate gene names: 21674272

Gene position: 1364208-1362391 (Counterclockwise)

Preceding gene: 21674273

Following gene: 21674271

Centisome position: 63.31

GC content: 57.48

Gene sequence:

>1818_bases
ATGCCTCCGACAGGAACAGAAGTCGGCATGATCAGAAACTTCTGCATTATTGCCCATATCGACCACGGCAAATCGACCCT
TGCCGACCGGCTTCTTGAAGTGACCCATACCCTCGAACGCAACCAGATGTCCACCGCCCAGGTGCTCGACGACATGGATC
TCGAACGCGAGCGCGGCATCACCATCAAGAGCCACGCGGTGCAGATGCGCTACACGGCCAAGGATGGGCAAGACTATATT
CTCAACCTCATTGACACGCCAGGTCACGTCGATTTCAGCTACGAGGTTTCGCGCTCGCTCGCCGCATGCGAAGGCGCGCT
GCTCGTTGTCGATGCCACGCAGGGCGTGGAGGCGCAAACCATCGCCAATCTCTACCTTGCCATTGAGGCAGGTCTCGAAA
TCATTCCGGTCATCAACAAGATCGACCTGCCATCGTCGGATGTCGAGGGCGTGGCGCGGCAGATCATCGACCTGATCGGC
GTCAACCGTGACGAAATTCTCCGGGTGTCGGCCAAGAATGGCATTGGCGTGGACGATCTCATGGAGGCGATCGTGGCGCG
CGTGCCCGCTCCGGCGGATAACCGCCAGATGCCGCTTCGTGCGCTCATCTTCGACTCGGTGTTCGATGCCTATCGTGGGG
CGATTGCCTATATCAGGATCGTGGACGGCGTGCTGAAGAAAGGAGACCGGGTGCGCTTTTTTGCTAACGACAAGATTTTC
ATGGCTGACGAAATCGGCACCATGAGTCTGAAGCGCAATCCGGTGGATATCCTCGAAGCGGGTAACGTAGGTTACCTGAT
CTGTTCGATCAAGGATGTCAAGGATGCCAAGGTCGGCGATACGGTCACGCTGGTTGAAAACCCGGCGGCGGAACGTCTTG
CCGGTTACAAGGATGTGAAACCGATGGTGTTCAGCGGTCTCTACCCGGTCGAATCGAACGAGTTCGAGGATCTGCGCGAG
TCGCTTGAAAAGCTCTCGCTCAACGATGCTTCGCTGGTCTATACGCCCGAGACCTCCGCAGCGCTCGGCTTTGGTTTCCG
CTGCGGCTTCCTCGGTCTGTTGCACATGGAGATCATCCAGGAGAGGCTGGAGCGCGAGTACGGCGTCAACATCATCACTA
CGGTGCCCAACGTTGAGTACCGCGTGATCATGACCAGCGGTGAGACGGTCGAGGTGGACAACCCCTCGAAGATGCCTGAG
ACGACCAAGATCAACTGGATCGAGGAGCCTTACGTGTCGATGCAGATCATCACCATGTCGGAGTACATCGGCAACATCAT
GAAGCTTGGCATGGAGCGTCGCGGCGAGTACAAGAACACCGACTATCTCGACACGTCGAGGGTGAACATTCATTTCGAGT
TCCCGCTGGGCGAGGTGGTGTTCGATTTCCACGACAAGCTCAAGTCGATCTCCAAAGGCTACGCCTCGATGGATTACGAG
TACATCGGCTACCGTCGTTCTGATCTGGTCAAGCTCGACGTGCTGCTCAACGGCGAGCCGGTCGATGCGCTGTCGTCGAT
CGTGCACCGCTCGAAATCCTACGAGTGGGGTCGCAAGCTTTGCCAGAAGCTCAAGGGGATCATCCCTCGCCAGATGTACG
AGGTGGCGATCCAGGCAGCCATCGGCAGCCGCGTGATCGCGCGCGAGAGTATTTCAGCCATGCGCAAGAACGTGCTTGCC
AAATGCTACGGCGGTGACATCAGCCGTAAGCGCAAGCTGCTTGAAAAGCAGAAAGAGGGCAAAAAGCGCATGAAGCAGGT
CGGCCGGGTCGAGGTGCCGCAGGAAGCCTTCCTCGCCGTTCTGAACATCGACGAATAA

Upstream 100 bases:

>100_bases
ACAATTTTCTTCTTATATTTTTTGATTTTATTATTCTACGCCCGCCGCTCTTGCGGCGAAGCGGTTGTGTAACCACTATT
ACTCGTTAGAACAGATACTT

Downstream 100 bases:

>100_bases
CAAGTTACAGACCCGTTTAATCAGCATGGCTGCGCTGGATACCACTCATCTTGACGTCTTACAGATGGTGCGTACGATTG
AGCATTCCAGCAACGTCTAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MPPTGTEVGMIRNFCIIAHIDHGKSTLADRLLEVTHTLERNQMSTAQVLDDMDLERERGITIKSHAVQMRYTAKDGQDYI
LNLIDTPGHVDFSYEVSRSLAACEGALLVVDATQGVEAQTIANLYLAIEAGLEIIPVINKIDLPSSDVEGVARQIIDLIG
VNRDEILRVSAKNGIGVDDLMEAIVARVPAPADNRQMPLRALIFDSVFDAYRGAIAYIRIVDGVLKKGDRVRFFANDKIF
MADEIGTMSLKRNPVDILEAGNVGYLICSIKDVKDAKVGDTVTLVENPAAERLAGYKDVKPMVFSGLYPVESNEFEDLRE
SLEKLSLNDASLVYTPETSAALGFGFRCGFLGLLHMEIIQERLEREYGVNIITTVPNVEYRVIMTSGETVEVDNPSKMPE
TTKINWIEEPYVSMQIITMSEYIGNIMKLGMERRGEYKNTDYLDTSRVNIHFEFPLGEVVFDFHDKLKSISKGYASMDYE
YIGYRRSDLVKLDVLLNGEPVDALSSIVHRSKSYEWGRKLCQKLKGIIPRQMYEVAIQAAIGSRVIARESISAMRKNVLA
KCYGGDISRKRKLLEKQKEGKKRMKQVGRVEVPQEAFLAVLNIDE

Sequences:

>Translated_605_residues
MPPTGTEVGMIRNFCIIAHIDHGKSTLADRLLEVTHTLERNQMSTAQVLDDMDLERERGITIKSHAVQMRYTAKDGQDYI
LNLIDTPGHVDFSYEVSRSLAACEGALLVVDATQGVEAQTIANLYLAIEAGLEIIPVINKIDLPSSDVEGVARQIIDLIG
VNRDEILRVSAKNGIGVDDLMEAIVARVPAPADNRQMPLRALIFDSVFDAYRGAIAYIRIVDGVLKKGDRVRFFANDKIF
MADEIGTMSLKRNPVDILEAGNVGYLICSIKDVKDAKVGDTVTLVENPAAERLAGYKDVKPMVFSGLYPVESNEFEDLRE
SLEKLSLNDASLVYTPETSAALGFGFRCGFLGLLHMEIIQERLEREYGVNIITTVPNVEYRVIMTSGETVEVDNPSKMPE
TTKINWIEEPYVSMQIITMSEYIGNIMKLGMERRGEYKNTDYLDTSRVNIHFEFPLGEVVFDFHDKLKSISKGYASMDYE
YIGYRRSDLVKLDVLLNGEPVDALSSIVHRSKSYEWGRKLCQKLKGIIPRQMYEVAIQAAIGSRVIARESISAMRKNVLA
KCYGGDISRKRKLLEKQKEGKKRMKQVGRVEVPQEAFLAVLNIDE
>Mature_604_residues
PPTGTEVGMIRNFCIIAHIDHGKSTLADRLLEVTHTLERNQMSTAQVLDDMDLERERGITIKSHAVQMRYTAKDGQDYIL
NLIDTPGHVDFSYEVSRSLAACEGALLVVDATQGVEAQTIANLYLAIEAGLEIIPVINKIDLPSSDVEGVARQIIDLIGV
NRDEILRVSAKNGIGVDDLMEAIVARVPAPADNRQMPLRALIFDSVFDAYRGAIAYIRIVDGVLKKGDRVRFFANDKIFM
ADEIGTMSLKRNPVDILEAGNVGYLICSIKDVKDAKVGDTVTLVENPAAERLAGYKDVKPMVFSGLYPVESNEFEDLRES
LEKLSLNDASLVYTPETSAALGFGFRCGFLGLLHMEIIQERLEREYGVNIITTVPNVEYRVIMTSGETVEVDNPSKMPET
TKINWIEEPYVSMQIITMSEYIGNIMKLGMERRGEYKNTDYLDTSRVNIHFEFPLGEVVFDFHDKLKSISKGYASMDYEY
IGYRRSDLVKLDVLLNGEPVDALSSIVHRSKSYEWGRKLCQKLKGIIPRQMYEVAIQAAIGSRVIARESISAMRKNVLAK
CYGGDISRKRKLLEKQKEGKKRMKQVGRVEVPQEAFLAVLNIDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=599, Percent_Identity=50.5843071786311, Blast_Score=644, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=45.4545454545455, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=41.2587412587413, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=43.1192660550459, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=43.1192660550459, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=43.1192660550459, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=38.6666666666667, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI217272892, Length=156, Percent_Identity=35.8974358974359, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI217272894, Length=156, Percent_Identity=35.8974358974359, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI25306283, Length=144, Percent_Identity=43.75, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI19923640, Length=144, Percent_Identity=43.75, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI25306287, Length=144, Percent_Identity=43.75, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI34147630, Length=283, Percent_Identity=27.208480565371, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI94966752, Length=132, Percent_Identity=32.5757575757576, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI53729339, Length=221, Percent_Identity=28.9592760180996, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=221, Percent_Identity=28.9592760180996, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=593, Percent_Identity=56.8296795952783, Blast_Score=673, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=478, Percent_Identity=30.7531380753138, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1789738, Length=162, Percent_Identity=37.037037037037, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=151, Percent_Identity=33.112582781457, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789559, Length=289, Percent_Identity=28.0276816608997, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789108, Length=148, Percent_Identity=31.7567567567568, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789737, Length=279, Percent_Identity=28.3154121863799, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790412, Length=279, Percent_Identity=28.3154121863799, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=44.0983606557377, Blast_Score=519, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71988811, Length=132, Percent_Identity=39.3939393939394, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=39.3939393939394, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17552882, Length=176, Percent_Identity=35.2272727272727, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=36.7741935483871, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556745, Length=158, Percent_Identity=36.0759493670886, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=138, Percent_Identity=40.5797101449275, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=288, Percent_Identity=28.125, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=235, Percent_Identity=30.2127659574468, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=46.8227424749164, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=40.8163265306122, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=40.8163265306122, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=142, Percent_Identity=42.2535211267606, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=39.7163120567376, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=41.0714285714286, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=45.9098497495826, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI28574573, Length=136, Percent_Identity=44.1176470588235, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=39.4557823129252, Blast_Score=100, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=39.4557823129252, Blast_Score=100, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=39.4557823129252, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24582462, Length=150, Percent_Identity=39.3333333333333, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=42.3841059602649, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357743, Length=159, Percent_Identity=33.9622641509434, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363316, Length=255, Percent_Identity=27.0588235294118, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17864358, Length=255, Percent_Identity=27.0588235294118, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28572034, Length=238, Percent_Identity=28.9915966386555, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921738, Length=287, Percent_Identity=26.1324041811847, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67769; Mature: 67638

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTGTEVGMIRNFCIIAHIDHGKSTLADRLLEVTHTLERNQMSTAQVLDDMDLERERGI
CCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCC
TIKSHAVQMRYTAKDGQDYILNLIDTPGHVDFSYEVSRSLAACEGALLVVDATQGVEAQT
EEEECEEEEEEECCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHH
IANLYLAIEAGLEIIPVINKIDLPSSDVEGVARQIIDLIGVNRDEILRVSAKNGIGVDDL
HHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHH
MEAIVARVPAPADNRQMPLRALIFDSVFDAYRGAIAYIRIVDGVLKKGDRVRFFANDKIF
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEE
MADEIGTMSLKRNPVDILEAGNVGYLICSIKDVKDAKVGDTVTLVENPAAERLAGYKDVK
EECCCCCEEECCCCHHHEECCCCEEEEEEECCCCCCCCCCEEEEECCCHHHHHCCCCCCC
PMVFSGLYPVESNEFEDLRESLEKLSLNDASLVYTPETSAALGFGFRCGFLGLLHMEIIQ
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHH
ERLEREYGVNIITTVPNVEYRVIMTSGETVEVDNPSKMPETTKINWIEEPYVSMQIITMS
HHHHHHHCCEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCEEEEECCCCCEEEEEEHH
EYIGNIMKLGMERRGEYKNTDYLDTSRVNIHFEFPLGEVVFDFHDKLKSISKGYASMDYE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCHH
YIGYRRSDLVKLDVLLNGEPVDALSSIVHRSKSYEWGRKLCQKLKGIIPRQMYEVAIQAA
HCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
IGSRVIARESISAMRKNVLAKCYGGDISRKRKLLEKQKEGKKRMKQVGRVEVPQEAFLAV
HCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEE
LNIDE
EECCC
>Mature Secondary Structure 
PPTGTEVGMIRNFCIIAHIDHGKSTLADRLLEVTHTLERNQMSTAQVLDDMDLERERGI
CCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCC
TIKSHAVQMRYTAKDGQDYILNLIDTPGHVDFSYEVSRSLAACEGALLVVDATQGVEAQT
EEEECEEEEEEECCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHH
IANLYLAIEAGLEIIPVINKIDLPSSDVEGVARQIIDLIGVNRDEILRVSAKNGIGVDDL
HHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEECCCCCCHHHH
MEAIVARVPAPADNRQMPLRALIFDSVFDAYRGAIAYIRIVDGVLKKGDRVRFFANDKIF
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEE
MADEIGTMSLKRNPVDILEAGNVGYLICSIKDVKDAKVGDTVTLVENPAAERLAGYKDVK
EECCCCCEEECCCCHHHEECCCCEEEEEEECCCCCCCCCCEEEEECCCHHHHHCCCCCCC
PMVFSGLYPVESNEFEDLRESLEKLSLNDASLVYTPETSAALGFGFRCGFLGLLHMEIIQ
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHH
ERLEREYGVNIITTVPNVEYRVIMTSGETVEVDNPSKMPETTKINWIEEPYVSMQIITMS
HHHHHHHCCEEEEECCCCEEEEEEECCCEEECCCCCCCCCCCEEEEECCCCCEEEEEEHH
EYIGNIMKLGMERRGEYKNTDYLDTSRVNIHFEFPLGEVVFDFHDKLKSISKGYASMDYE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCHH
YIGYRRSDLVKLDVLLNGEPVDALSSIVHRSKSYEWGRKLCQKLKGIIPRQMYEVAIQAA
HCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
IGSRVIARESISAMRKNVLAKCYGGDISRKRKLLEKQKEGKKRMKQVGRVEVPQEAFLAV
HCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEE
LNIDE
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA