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The map label for this gene is greA [H]

Identifier: 21674267

GI number: 21674267

Start: 1357167

End: 1357646

Strand: Reverse

Name: greA [H]

Synonym: CT1446

Alternate gene names: 21674267

Gene position: 1357646-1357167 (Counterclockwise)

Preceding gene: 21674268

Following gene: 21674266

Centisome position: 63.0

GC content: 47.92

Gene sequence:

>480_bases
ATGAGTGATCGAATTTATCTGACACGAGATGGGTATAATCGGCTCAAGGAGGAACTTCACTTGTTGATGACCGAGACCCG
CAAGGAGGTGCTTGAAAAGATTGCCGAAGCTCGCTCGCATGGAGACCTGAGTGAAAACGCCGAGTATGACGCTGCCCGAG
AAGAACAGTCGCAACTTGAAGCGAAAATCGGTGAAATCGAAAATAAACTGGCATCAGCTACTATCCTCGATCCCAAGCAG
ATCAAGACCGATCGGGTTTATATTCTCACATCGGTAAAGCTACGTAATCTGGATGCCGAGGACGAAATTATTGAATACAC
CCTTGTCTCGTCCGAGGAGGCCGATTCCGATCTTGGCAAAATATCCGTGCGTTCTCCCGTCGGCCGGTCGCTGATTGGCA
AGTCGGTTGGTGACAAGGTTACAATCAGCGTGCCGAAAGGTGAGCTCCATTATGAAATCCTCGATATTTTTGTTAAATAA

Upstream 100 bases:

>100_bases
AGGCTGCTGACTTTACTTTTTTTGTTTGCCAAACCATTGTTGCTGTAACGTCTGGTGATCATTCGCTTTTCTGCATCACC
AATCAAAACCATGAACTGAC

Downstream 100 bases:

>100_bases
AAAGCCAATTCAAACACCATATGTATGGGAAAACGTCAGATAATTTATACTGCCAGCCAGATCGGTGGAGCCCGTGAATT
GCTCGACAAGGAAATCAATC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSDRIYLTRDGYNRLKEELHLLMTETRKEVLEKIAEARSHGDLSENAEYDAAREEQSQLEAKIGEIENKLASATILDPKQ
IKTDRVYILTSVKLRNLDAEDEIIEYTLVSSEEADSDLGKISVRSPVGRSLIGKSVGDKVTISVPKGELHYEILDIFVK

Sequences:

>Translated_159_residues
MSDRIYLTRDGYNRLKEELHLLMTETRKEVLEKIAEARSHGDLSENAEYDAAREEQSQLEAKIGEIENKLASATILDPKQ
IKTDRVYILTSVKLRNLDAEDEIIEYTLVSSEEADSDLGKISVRSPVGRSLIGKSVGDKVTISVPKGELHYEILDIFVK
>Mature_158_residues
SDRIYLTRDGYNRLKEELHLLMTETRKEVLEKIAEARSHGDLSENAEYDAAREEQSQLEAKIGEIENKLASATILDPKQI
KTDRVYILTSVKLRNLDAEDEIIEYTLVSSEEADSDLGKISVRSPVGRSLIGKSVGDKVTISVPKGELHYEILDIFVK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=43.1372549019608, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI87082261, Length=147, Percent_Identity=30.6122448979592, Blast_Score=76, Evalue=8e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17950; Mature: 17818

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRIYLTRDGYNRLKEELHLLMTETRKEVLEKIAEARSHGDLSENAEYDAAREEQSQLE
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AKIGEIENKLASATILDPKQIKTDRVYILTSVKLRNLDAEDEIIEYTLVSSEEADSDLGK
HHHHHHHHHHHHCCCCCHHHCCCCEEEEEEEEEEECCCCCHHHEEEEEECCCCCCCCCCE
ISVRSPVGRSLIGKSVGDKVTISVPKGELHYEILDIFVK
EEECCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SDRIYLTRDGYNRLKEELHLLMTETRKEVLEKIAEARSHGDLSENAEYDAAREEQSQLE
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
AKIGEIENKLASATILDPKQIKTDRVYILTSVKLRNLDAEDEIIEYTLVSSEEADSDLGK
HHHHHHHHHHHHCCCCCHHHCCCCEEEEEEEEEEECCCCCHHHEEEEEECCCCCCCCCCE
ISVRSPVGRSLIGKSVGDKVTISVPKGELHYEILDIFVK
EEECCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA