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The map label for this gene is aroK

Identifier: 21674226

GI number: 21674226

Start: 1318344

End: 1318919

Strand: Direct

Name: aroK

Synonym: CT1405

Alternate gene names: 21674226

Gene position: 1318344-1318919 (Clockwise)

Preceding gene: 21674225

Following gene: 21674227

Centisome position: 61.18

GC content: 59.2

Gene sequence:

>576_bases
ATGAAACACCACTCACTCATATTTCTGACCGGCTTCAGCGGTTCAGGCAAATCGACCATCGGCCCGCTCCTGGCCAACTC
GCTCGGCTTCGAGTTCATTGACCTCGACCGCGAAATCGAGCTTACCGCCGGGAAGAGCATCAACCGGATTTTCGCCGAAG
ATGGCGAGGCCGCCTTCCGCTCGCTCGAACTCCGGACGCTCGAAAAGATCGGCCAACAGGAGCGGATGGTGGTCTCGCTT
GGCGGCGGCGTGCTCGAAAACGACCGATGCTTCGAGCTGATCCGCTCGCACGGCACCCTGATCTACCTCAAGTCCAGCCC
TGAAATTCTCACGCTCCGGCTCCAGCACAAGACCGACCGCCCCCTGCTCAAAGGCCCGGACGGGCGGAAGCTGACGCGAG
AAGAGATCCAGCAGCGCATCGCGGAGCTTCTGAAAAAACGGGAACCCCGCTACCTGAAAGCCGATCTGGTGCTCTTCACC
GACTCGAAAAAGATCGGCGCAAGTGTCGAGGAGTTGACACGCAAAATCGAGCGGCACATCCGCCGCGCGTCGAAAAACAA
CACTAACGAGAAGTAA

Upstream 100 bases:

>100_bases
GCGAAAACAATCGCCCTGAGCCGGTTATCGCTCATTTTTCGTATATTGGCCGCAAATCAACAGCTGGCTAGCTGGCTGCC
AGCGCCTGCCGAATCACAGG

Downstream 100 bases:

>100_bases
GACATGCAGACTCCGTCCAGCCATATCATCGTCAGAACGCCGGTCATCGACTCCGTCGGTGAACTCTACGCCACAAGGGG
ACTCGGCAAGAAAACCGTGC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKHHSLIFLTGFSGSGKSTIGPLLANSLGFEFIDLDREIELTAGKSINRIFAEDGEAAFRSLELRTLEKIGQQERMVVSL
GGGVLENDRCFELIRSHGTLIYLKSSPEILTLRLQHKTDRPLLKGPDGRKLTREEIQQRIAELLKKREPRYLKADLVLFT
DSKKIGASVEELTRKIERHIRRASKNNTNEK

Sequences:

>Translated_191_residues
MKHHSLIFLTGFSGSGKSTIGPLLANSLGFEFIDLDREIELTAGKSINRIFAEDGEAAFRSLELRTLEKIGQQERMVVSL
GGGVLENDRCFELIRSHGTLIYLKSSPEILTLRLQHKTDRPLLKGPDGRKLTREEIQQRIAELLKKREPRYLKADLVLFT
DSKKIGASVEELTRKIERHIRRASKNNTNEK
>Mature_191_residues
MKHHSLIFLTGFSGSGKSTIGPLLANSLGFEFIDLDREIELTAGKSINRIFAEDGEAAFRSLELRTLEKIGQQERMVVSL
GGGVLENDRCFELIRSHGTLIYLKSSPEILTLRLQHKTDRPLLKGPDGRKLTREEIQQRIAELLKKREPRYLKADLVLFT
DSKKIGASVEELTRKIERHIRRASKNNTNEK

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=164, Percent_Identity=31.0975609756098, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1786587, Length=177, Percent_Identity=26.5536723163842, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_CHLTE (Q8KCL0)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662291.1
- ProteinModelPortal:   Q8KCL0
- SMR:   Q8KCL0
- GeneID:   1006814
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1405
- NMPDR:   fig|194439.1.peg.1385
- TIGR:   CT1405
- HOGENOM:   HBG335815
- OMA:   MIISERV
- ProtClustDB:   PRK00131
- BioCyc:   CTEP194439:CT_1405-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 21695; Mature: 21695

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 36-36 BINDING 60-60 BINDING 82-82 BINDING 120-120 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHHSLIFLTGFSGSGKSTIGPLLANSLGFEFIDLDREIELTAGKSINRIFAEDGEAAFR
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHCCCHHHHH
SLELRTLEKIGQQERMVVSLGGGVLENDRCFELIRSHGTLIYLKSSPEILTLRLQHKTDR
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCC
PLLKGPDGRKLTREEIQQRIAELLKKREPRYLKADLVLFTDSKKIGASVEELTRKIERHI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
RRASKNNTNEK
HHHHCCCCCCC
>Mature Secondary Structure
MKHHSLIFLTGFSGSGKSTIGPLLANSLGFEFIDLDREIELTAGKSINRIFAEDGEAAFR
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHCCCHHHHH
SLELRTLEKIGQQERMVVSLGGGVLENDRCFELIRSHGTLIYLKSSPEILTLRLQHKTDR
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCC
PLLKGPDGRKLTREEIQQRIAELLKKREPRYLKADLVLFTDSKKIGASVEELTRKIERHI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
RRASKNNTNEK
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901