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The map label for this gene is rimO

Identifier: 21674219

GI number: 21674219

Start: 1311309

End: 1312613

Strand: Direct

Name: rimO

Synonym: CT1398

Alternate gene names: 21674219

Gene position: 1311309-1312613 (Clockwise)

Preceding gene: 21674218

Following gene: 21674222

Centisome position: 60.85

GC content: 58.77

Gene sequence:

>1305_bases
ATGACAAAAACTGACGAACGCAAACCGGCAATCTTCCTGCTCAGCCTCGGCTGCTCGAAGAACACGGTCGATAGTGAGCG
ACTCACCGCGCAGGCGGTCGCCTCCGGCCTGACCTTTACTGACAATGTTGACGAAGCGGACATCATCCTCATCAACACCT
GTGGTTTCATCAAGGATGCCAAACAGGAGTCGATCGACGAAACACTCGCCGCTATCGGCAAGAAGGAGGAGGGGGTCGTC
CGCGAAGTCTATGTGATGGGGTGCCTGGTGGAACTCTACCGCAAGGAACTCGCCGAAGAGATGCCGGAAATCGACGGTCT
CTTTGGCACCCGCGAACTTCCGGAAGTGCTGGCAGCCATTGGCGCAAAGTACCGCGAGGAGCTGTTCGACCGGCGCGAAC
TGCTCACGCCGCCGCACTACGCTTTCCTGAAAATCGCCGAAGGGTGCAACCGCCGCTGCTCCTTCTGCTCGATCCCGAAA
ATCCGAGGCCCCTACGTCAGCCAGCCCATCGAGCAGCTTCTGCGCGAAGCCGCGCTGCTCCAGCAGCAGGGCGTCAAGGA
GCTGAACCTCATCGCACAAGACATCAGCGTGTACGGCTACGACCTCTACGGCAAGTCCGCGCTCAACGACCTCACGCTGC
GCCTCTCCGACATGGGCTTCAACTGGATCCGCCTGCTCTACGCCTACCCGCTCAACTTCCCGCTGGAGGTGATCAGCACC
ATGCGCGAACGTCCGAACGTCTGCAACTACATCGATATGCCGCTGCAGCACATCAACGACCGCATCTTGAAATCGATGCA
GCGCGGCATTGGGCGCAAGGCCACCGAACAGCTCATCGACGACATCCGCCAGAAGAACCCCGACATCCGCCTCCGCACCA
CCATGATCGCCGGCTACCCCGGCGAAACCCGCGCAGAGTTTGAGGAACTGCTCGACTTCATCCGACAGACGCGCTTCGAC
CGCCTCGGCTGCTTCCCCTACCGCCACGAAGAGCACGCCTCTGCCTACGCGCTTGAAGACACGGTCAGCGACGAGGAAAA
GGAGAAACGAGTCGGCGAACTGATGGAACTCCAGGAAGGAATTTCGGCCAGCCTCAACCGCAAACTCGAAGGCCAGACGC
TGAAAGTACTCATCGACCGGATTGAGGAAAGCGTCGCCTACGCAAGGACAGAATACGACGCCCCCGAAGTCGATAACGAC
GTGATTATCGAGATTGGAGATGAAGCTGTGGAAGAAGGCGATTTCCGACAGGTGATGATCGAGGATTCAACGGCGTATGA
GTTGTTTGGGAGAATTAGCGGGTAG

Upstream 100 bases:

>100_bases
CTCTCGTTCTACCTCTGGCTCACCAGAACGGCACGCGAGCACATCGCGGCAGGCGACTTCACCGACTGGAAAGAGTCATT
CCTCATGGAGTTCAACAGCA

Downstream 100 bases:

>100_bases
AAATGAAAGTAAATCGCGCTGAAAGCTCATGCCGAATCTTTCAGTGCGGGTTACAGCATACGGTCAAGCCTCTGGTTCAA
TCTTCAGTTTCTCTCTGATA

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTKTDERKPAIFLLSLGCSKNTVDSERLTAQAVASGLTFTDNVDEADIILINTCGFIKDAKQESIDETLAAIGKKEEGVV
REVYVMGCLVELYRKELAEEMPEIDGLFGTRELPEVLAAIGAKYREELFDRRELLTPPHYAFLKIAEGCNRRCSFCSIPK
IRGPYVSQPIEQLLREAALLQQQGVKELNLIAQDISVYGYDLYGKSALNDLTLRLSDMGFNWIRLLYAYPLNFPLEVIST
MRERPNVCNYIDMPLQHINDRILKSMQRGIGRKATEQLIDDIRQKNPDIRLRTTMIAGYPGETRAEFEELLDFIRQTRFD
RLGCFPYRHEEHASAYALEDTVSDEEKEKRVGELMELQEGISASLNRKLEGQTLKVLIDRIEESVAYARTEYDAPEVDND
VIIEIGDEAVEEGDFRQVMIEDSTAYELFGRISG

Sequences:

>Translated_434_residues
MTKTDERKPAIFLLSLGCSKNTVDSERLTAQAVASGLTFTDNVDEADIILINTCGFIKDAKQESIDETLAAIGKKEEGVV
REVYVMGCLVELYRKELAEEMPEIDGLFGTRELPEVLAAIGAKYREELFDRRELLTPPHYAFLKIAEGCNRRCSFCSIPK
IRGPYVSQPIEQLLREAALLQQQGVKELNLIAQDISVYGYDLYGKSALNDLTLRLSDMGFNWIRLLYAYPLNFPLEVIST
MRERPNVCNYIDMPLQHINDRILKSMQRGIGRKATEQLIDDIRQKNPDIRLRTTMIAGYPGETRAEFEELLDFIRQTRFD
RLGCFPYRHEEHASAYALEDTVSDEEKEKRVGELMELQEGISASLNRKLEGQTLKVLIDRIEESVAYARTEYDAPEVDND
VIIEIGDEAVEEGDFRQVMIEDSTAYELFGRISG
>Mature_433_residues
TKTDERKPAIFLLSLGCSKNTVDSERLTAQAVASGLTFTDNVDEADIILINTCGFIKDAKQESIDETLAAIGKKEEGVVR
EVYVMGCLVELYRKELAEEMPEIDGLFGTRELPEVLAAIGAKYREELFDRRELLTPPHYAFLKIAEGCNRRCSFCSIPKI
RGPYVSQPIEQLLREAALLQQQGVKELNLIAQDISVYGYDLYGKSALNDLTLRLSDMGFNWIRLLYAYPLNFPLEVISTM
RERPNVCNYIDMPLQHINDRILKSMQRGIGRKATEQLIDDIRQKNPDIRLRTTMIAGYPGETRAEFEELLDFIRQTRFDR
LGCFPYRHEEHASAYALEDTVSDEEKEKRVGELMELQEGISASLNRKLEGQTLKVLIDRIEESVAYARTEYDAPEVDNDV
IIEIGDEAVEEGDFRQVMIEDSTAYELFGRISG

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=423, Percent_Identity=26.9503546099291, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI28872782, Length=423, Percent_Identity=26.9503546099291, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI93277076, Length=339, Percent_Identity=24.188790560472, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1787057, Length=442, Percent_Identity=39.5927601809955, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1786882, Length=390, Percent_Identity=29.2307692307692, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=25.6900212314225, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71996771, Length=279, Percent_Identity=27.9569892473118, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356207, Length=472, Percent_Identity=26.0593220338983, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922432, Length=337, Percent_Identity=24.3323442136499, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CHLTE (Q8KCL7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662284.1
- ProteinModelPortal:   Q8KCL7
- SMR:   Q8KCL7
- GeneID:   1006804
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1398
- NMPDR:   fig|194439.1.peg.1378
- TIGR:   CT1398
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK637740
- BioCyc:   CTEP194439:CT_1398-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49411; Mature: 49280

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTDERKPAIFLLSLGCSKNTVDSERLTAQAVASGLTFTDNVDEADIILINTCGFIKDA
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHH
KQESIDETLAAIGKKEEGVVREVYVMGCLVELYRKELAEEMPEIDGLFGTRELPEVLAAI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHH
GAKYREELFDRRELLTPPHYAFLKIAEGCNRRCSFCSIPKIRGPYVSQPIEQLLREAALL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QQQGVKELNLIAQDISVYGYDLYGKSALNDLTLRLSDMGFNWIRLLYAYPLNFPLEVIST
HHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
MRERPNVCNYIDMPLQHINDRILKSMQRGIGRKATEQLIDDIRQKNPDIRLRTTMIAGYP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEECCC
GETRAEFEELLDFIRQTRFDRLGCFPYRHEEHASAYALEDTVSDEEKEKRVGELMELQEG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
ISASLNRKLEGQTLKVLIDRIEESVAYARTEYDAPEVDNDVIIEIGDEAVEEGDFRQVMI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHCCCCCEEEEE
EDSTAYELFGRISG
CCCHHHHHHHHCCC
>Mature Secondary Structure 
TKTDERKPAIFLLSLGCSKNTVDSERLTAQAVASGLTFTDNVDEADIILINTCGFIKDA
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHH
KQESIDETLAAIGKKEEGVVREVYVMGCLVELYRKELAEEMPEIDGLFGTRELPEVLAAI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHH
GAKYREELFDRRELLTPPHYAFLKIAEGCNRRCSFCSIPKIRGPYVSQPIEQLLREAALL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QQQGVKELNLIAQDISVYGYDLYGKSALNDLTLRLSDMGFNWIRLLYAYPLNFPLEVIST
HHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHH
MRERPNVCNYIDMPLQHINDRILKSMQRGIGRKATEQLIDDIRQKNPDIRLRTTMIAGYP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEECCC
GETRAEFEELLDFIRQTRFDRLGCFPYRHEEHASAYALEDTVSDEEKEKRVGELMELQEG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
ISASLNRKLEGQTLKVLIDRIEESVAYARTEYDAPEVDNDVIIEIGDEAVEEGDFRQVMI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHCCCCCEEEEE
EDSTAYELFGRISG
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901