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The map label for this gene is tgt [H]

Identifier: 21674218

GI number: 21674218

Start: 1310189

End: 1311322

Strand: Direct

Name: tgt [H]

Synonym: CT1397

Alternate gene names: 21674218

Gene position: 1310189-1311322 (Clockwise)

Preceding gene: 21674216

Following gene: 21674219

Centisome position: 60.8

GC content: 59.08

Gene sequence:

>1134_bases
ATGAAGTTTTCAGTTCTCTCGACAGACCGCCACTCAGCCGCGCGTTGCGGCGTGCTCTCAACCAGCCACGGCGACATTCC
AACGCCGATTTTCATGCCGGTTGGTACGCGGGCGAGCGTCAAATCAGTCGAGCCGAACGAGCTGAAAGCGCTCGATGCGA
AGATCATTCTGGCCAACACTTACCACCTCTATCTGAAACCCGGCAACGATATACTTTTCAAGGCTGGAGGCGTGCACCGT
TTCATGAACTGGGATGGCCCGTTGTTGACCGACAGCGGCGGCTATCAGGTCTATTCGCTGACCGATCTGCGGAAAATCAG
CGAAGAGGGGGTGATGTTCAAGTCGCACCTCGACGGCTCGAAACTCCACTTTACACCGGAAAACGTCGTTGATACGCAAC
GTATTATCGGCAGCGACATCATGATGCCGCTTGACGAATGCCCGCCGTGGCCTGCCGAAAAGGAGTATGTGCAGAAGTCG
GGCGAGCTGACGCTGCGCTGGGCCGAGCGGGCACGAAAGCACTTCCTCTCGACCCGACCGCACTACGGCTACGAACAGTT
CCAGTTCGGCATCACGCAAGGCGGCACCTTCGACGACCTGCGTGCCCACTCCAGCCGGGCGCTCGTCGATATGGATTTTG
ACGGCTACGCGGTCGGCGGCATGGCGGTCGGCGAACCTGCGGAGGAGATGTACCGGATGCTTGAGCTATCGCACACCATT
TTGCCCGAATCGAAGCCGCGCTACCTGATGGGCGTCGGCACGCCCGCGAACATTCTCAACGCTATCGAGCGCGGCATCGA
CATGTTCGACTGCGTCATCCCCACGCGCGAGGGTCGCAACGGCCGCGTCTATACCCGCAAAGGGACGATCAACCTGCGTG
CGGGCAAGTATGCCGACGACTTCCGTCCCATCGACGAAGGCTTCGACAACCACGTCTGCCGCAACTACAGCCGCGCCTAC
ATCCGCCACCTGCTCAACGTGGGCGAAATTCTCGGCCTCAAGCTCTGCACCATGCAGAACCTCTCGTTCTACCTCTGGCT
CACCAGAACGGCACGCGAGCACATCGCGGCAGGCGACTTCACCGACTGGAAAGAGTCATTCCTCATGGAGTTCAACAGCA
ATGACAAAAACTGA

Upstream 100 bases:

>100_bases
CGAACACGCGAAGCGTACCAATTTTTATTTGTGAATACGGTTCCCTACCTTCATTCATCGTTATCACCACTCTATCCGTT
TTTGCCACCCGCGCATCAAT

Downstream 100 bases:

>100_bases
CGAACGCAAACCGGCAATCTTCCTGCTCAGCCTCGGCTGCTCGAAGAACACGGTCGATAGTGAGCGACTCACCGCGCAGG
CGGTCGCCTCCGGCCTGACC

Product: tRNA-guanine transglycosylase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKFSVLSTDRHSAARCGVLSTSHGDIPTPIFMPVGTRASVKSVEPNELKALDAKIILANTYHLYLKPGNDILFKAGGVHR
FMNWDGPLLTDSGGYQVYSLTDLRKISEEGVMFKSHLDGSKLHFTPENVVDTQRIIGSDIMMPLDECPPWPAEKEYVQKS
GELTLRWAERARKHFLSTRPHYGYEQFQFGITQGGTFDDLRAHSSRALVDMDFDGYAVGGMAVGEPAEEMYRMLELSHTI
LPESKPRYLMGVGTPANILNAIERGIDMFDCVIPTREGRNGRVYTRKGTINLRAGKYADDFRPIDEGFDNHVCRNYSRAY
IRHLLNVGEILGLKLCTMQNLSFYLWLTRTAREHIAAGDFTDWKESFLMEFNSNDKN

Sequences:

>Translated_377_residues
MKFSVLSTDRHSAARCGVLSTSHGDIPTPIFMPVGTRASVKSVEPNELKALDAKIILANTYHLYLKPGNDILFKAGGVHR
FMNWDGPLLTDSGGYQVYSLTDLRKISEEGVMFKSHLDGSKLHFTPENVVDTQRIIGSDIMMPLDECPPWPAEKEYVQKS
GELTLRWAERARKHFLSTRPHYGYEQFQFGITQGGTFDDLRAHSSRALVDMDFDGYAVGGMAVGEPAEEMYRMLELSHTI
LPESKPRYLMGVGTPANILNAIERGIDMFDCVIPTREGRNGRVYTRKGTINLRAGKYADDFRPIDEGFDNHVCRNYSRAY
IRHLLNVGEILGLKLCTMQNLSFYLWLTRTAREHIAAGDFTDWKESFLMEFNSNDKN
>Mature_377_residues
MKFSVLSTDRHSAARCGVLSTSHGDIPTPIFMPVGTRASVKSVEPNELKALDAKIILANTYHLYLKPGNDILFKAGGVHR
FMNWDGPLLTDSGGYQVYSLTDLRKISEEGVMFKSHLDGSKLHFTPENVVDTQRIIGSDIMMPLDECPPWPAEKEYVQKS
GELTLRWAERARKHFLSTRPHYGYEQFQFGITQGGTFDDLRAHSSRALVDMDFDGYAVGGMAVGEPAEEMYRMLELSHTI
LPESKPRYLMGVGTPANILNAIERGIDMFDCVIPTREGRNGRVYTRKGTINLRAGKYADDFRPIDEGFDNHVCRNYSRAY
IRHLLNVGEILGLKLCTMQNLSFYLWLTRTAREHIAAGDFTDWKESFLMEFNSNDKN

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=357, Percent_Identity=39.7759103641457, Blast_Score=267, Evalue=1e-71,
Organism=Escherichia coli, GI1786607, Length=371, Percent_Identity=47.7088948787062, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17544680, Length=372, Percent_Identity=35.4838709677419, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI25146502, Length=171, Percent_Identity=27.4853801169591, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=368, Percent_Identity=39.1304347826087, Blast_Score=281, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42662; Mature: 42662

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSVLSTDRHSAARCGVLSTSHGDIPTPIFMPVGTRASVKSVEPNELKALDAKIILANT
CEEEEECCCCCCHHHCCEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHEECEEEEEEEE
YHLYLKPGNDILFKAGGVHRFMNWDGPLLTDSGGYQVYSLTDLRKISEEGVMFKSHLDGS
EEEEEECCCEEEEECCCEEEEECCCCCEEECCCCEEEEEEHHHHHHHHCCCEEEECCCCC
KLHFTPENVVDTQRIIGSDIMMPLDECPPWPAEKEYVQKSGELTLRWAERARKHFLSTRP
EEEECCHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHCCCC
HYGYEQFQFGITQGGTFDDLRAHSSRALVDMDFDGYAVGGMAVGEPAEEMYRMLELSHTI
CCCHHHEEEECCCCCCHHHHHHCCCCEEEEECCCCEEECCEECCCCHHHHHHHHHHHHHH
LPESKPRYLMGVGTPANILNAIERGIDMFDCVIPTREGRNGRVYTRKGTINLRAGKYADD
CCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCEEEEECCCCCCC
FRPIDEGFDNHVCRNYSRAYIRHLLNVGEILGLKLCTMQNLSFYLWLTRTAREHIAAGDF
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEEHHHHHHHCCCCC
TDWKESFLMEFNSNDKN
CHHHHHHHEEECCCCCC
>Mature Secondary Structure
MKFSVLSTDRHSAARCGVLSTSHGDIPTPIFMPVGTRASVKSVEPNELKALDAKIILANT
CEEEEECCCCCCHHHCCEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHEECEEEEEEEE
YHLYLKPGNDILFKAGGVHRFMNWDGPLLTDSGGYQVYSLTDLRKISEEGVMFKSHLDGS
EEEEEECCCEEEEECCCEEEEECCCCCEEECCCCEEEEEEHHHHHHHHCCCEEEECCCCC
KLHFTPENVVDTQRIIGSDIMMPLDECPPWPAEKEYVQKSGELTLRWAERARKHFLSTRP
EEEECCHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHCCCC
HYGYEQFQFGITQGGTFDDLRAHSSRALVDMDFDGYAVGGMAVGEPAEEMYRMLELSHTI
CCCHHHEEEECCCCCCHHHHHHCCCCEEEEECCCCEEECCEECCCCHHHHHHHHHHHHHH
LPESKPRYLMGVGTPANILNAIERGIDMFDCVIPTREGRNGRVYTRKGTINLRAGKYADD
CCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCEEEEECCCCCCC
FRPIDEGFDNHVCRNYSRAYIRHLLNVGEILGLKLCTMQNLSFYLWLTRTAREHIAAGDF
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEEHHHHHHHCCCCC
TDWKESFLMEFNSNDKN
CHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA