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The map label for this gene is oprF [H]

Identifier: 21674175

GI number: 21674175

Start: 1272160

End: 1272855

Strand: Direct

Name: oprF [H]

Synonym: CT1353

Alternate gene names: 21674175

Gene position: 1272160-1272855 (Clockwise)

Preceding gene: 21674173

Following gene: 21674176

Centisome position: 59.03

GC content: 59.63

Gene sequence:

>696_bases
ATGACAATGAAAACGATCAAGAAATTCTCGAAACCGGCGGCCTTGCTCCTCCTTGCTTCGACAGCTACTGTCACCACCGG
CTGCCAGTCCACGACAAACGCCGGGCGCGGTGCCGGGTACGGTGCGGCAGCCGGCGGCTTGATCGGCGGCATTATCGGCA
GCAACAACGGCAGCTGGGTTCAGGGAGCGCTGATCGGCGCTGCCATCGGCGGCGCGGCTGGCGCGGTCATCGGCGACTAC
ATGGACAAGCAGGCCGACGAAATTCGCCAGGAGGTTCAGGGAGCGAAGGTCGAGCGAGTCGGCGAAGGCATCCGCGTGGT
TTTCGATACCGGCCTGCTCTTCTCGACCGACTCGGCAACCCTCAACGCCAACAGCCGTTACAACATCGAGAAACTTGCAA
GAATCCTGAACCGCTACAACGATACTAACGTGGTCATTGAAGGGCATACCGACAATACCGGCACAGAAGCCTCGAACCAG
ATTCTTTCCGAACGACGCGCCGAATCGGTTGCGACGCTTCTGAGAACCTATGGCGTCTCTGGCCGCCGTCTCACTGCAAT
CGGATACGGCGAAACCCGACCGGTAGCCACCAATGAAACCGAGGCAGGCAGACGCCTGAACCGCCGCGTCGAAGTACTGA
TTTACGCCAACGACGCTCTGAAGCGACAAGCCCAGGCCGGAGAACTAAAGCTTTGA

Upstream 100 bases:

>100_bases
TTCATAAATTTGCTCAAGAGACGCATCTGGTCATTCAGTATCGGTTGCGGCTAATCTGATAGCAATAGCACATGATATTT
TTTTTGAACCCCAAACCATG

Downstream 100 bases:

>100_bases
TTGTCTTGGTTCAAACCAAGGTTTGCCAAAACTAAAAAAATGCTTATTTTTAGCCCGTCCGGCTTGCTCAGAGATGCGTC
TGAGTCATGCCCGACGGGCA

Product: OmpA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTMKTIKKFSKPAALLLLASTATVTTGCQSTTNAGRGAGYGAAAGGLIGGIIGSNNGSWVQGALIGAAIGGAAGAVIGDY
MDKQADEIRQEVQGAKVERVGEGIRVVFDTGLLFSTDSATLNANSRYNIEKLARILNRYNDTNVVIEGHTDNTGTEASNQ
ILSERRAESVATLLRTYGVSGRRLTAIGYGETRPVATNETEAGRRLNRRVEVLIYANDALKRQAQAGELKL

Sequences:

>Translated_231_residues
MTMKTIKKFSKPAALLLLASTATVTTGCQSTTNAGRGAGYGAAAGGLIGGIIGSNNGSWVQGALIGAAIGGAAGAVIGDY
MDKQADEIRQEVQGAKVERVGEGIRVVFDTGLLFSTDSATLNANSRYNIEKLARILNRYNDTNVVIEGHTDNTGTEASNQ
ILSERRAESVATLLRTYGVSGRRLTAIGYGETRPVATNETEAGRRLNRRVEVLIYANDALKRQAQAGELKL
>Mature_230_residues
TMKTIKKFSKPAALLLLASTATVTTGCQSTTNAGRGAGYGAAAGGLIGGIIGSNNGSWVQGALIGAAIGGAAGAVIGDYM
DKQADEIRQEVQGAKVERVGEGIRVVFDTGLLFSTDSATLNANSRYNIEKLARILNRYNDTNVVIEGHTDNTGTEASNQI
LSERRAESVATLLRTYGVSGRRLTAIGYGETRPVATNETEAGRRLNRRVEVLIYANDALKRQAQAGELKL

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=137, Percent_Identity=36.4963503649635, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787191, Length=126, Percent_Identity=32.5396825396825, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 24335; Mature: 24204

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKTIKKFSKPAALLLLASTATVTTGCQSTTNAGRGAGYGAAAGGLIGGIIGSNNGSWV
CCHHHHHHHCCCEEEEEEECCHHEECCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCEE
QGALIGAAIGGAAGAVIGDYMDKQADEIRQEVQGAKVERVGEGIRVVFDTGLLFSTDSAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCEEEECCCCE
LNANSRYNIEKLARILNRYNDTNVVIEGHTDNTGTEASNQILSERRAESVATLLRTYGVS
ECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GRRLTAIGYGETRPVATNETEAGRRLNRRVEVLIYANDALKRQAQAGELKL
CCEEEEEECCCCCCCCCCCHHHHHHHCCEEEEEEEECHHHHHHHCCCCCCC
>Mature Secondary Structure 
TMKTIKKFSKPAALLLLASTATVTTGCQSTTNAGRGAGYGAAAGGLIGGIIGSNNGSWV
CHHHHHHHCCCEEEEEEECCHHEECCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCEE
QGALIGAAIGGAAGAVIGDYMDKQADEIRQEVQGAKVERVGEGIRVVFDTGLLFSTDSAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCEEEECCCCE
LNANSRYNIEKLARILNRYNDTNVVIEGHTDNTGTEASNQILSERRAESVATLLRTYGVS
ECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GRRLTAIGYGETRPVATNETEAGRRLNRRVEVLIYANDALKRQAQAGELKL
CCEEEEEECCCCCCCCCCCHHHHHHHCCEEEEEEEECHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]