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The map label for this gene is ispD

Identifier: 21674140

GI number: 21674140

Start: 1240042

End: 1240782

Strand: Direct

Name: ispD

Synonym: CT1317

Alternate gene names: 21674140

Gene position: 1240042-1240782 (Clockwise)

Preceding gene: 21674139

Following gene: 21674141

Centisome position: 57.54

GC content: 56.68

Gene sequence:

>741_bases
ATGAAAACAGTAGTCATCATTGCGGCAAGCGGCGTCGGCAAACGCATGAAGCTCGATGGCGGCCGCAGCAAGCAGATGCT
TGAAATCGGCGGCCAGCCGGTCATCTGGCACACCATGAAAGCGTTTCAGGAGGCATCGACGGTCGAATCGGTTTACATCG
CCACGCTGCCAGACAGCATCCCGGTTTTCAAGGAGATTGCGAAAGCAAACGGATTCACGAAGATCACAGCAATAATCGAA
GGCGGCAAGGAGCGGCAGGACTCGATCGGTAATTGCATGAAGCTGATCGAACAAGAGATTGAGAACTCGGGAGTGATGCC
CGACGCCATCCTCGTGCACGACGGCGCACGCCCGTTTATCCAGCCGGAGGAGATCGACGACATCGCGCGTCTGTCGGCGA
CACACGGCGCATGCGTGCCCGCCACAAAACCGAAAGATACCATCAAGTATGTCGGCTGCAATCCGGAGATTTTCGGCGAA
ACGCTCGACCGCAGCCGCCTGCTCCAGGTACAGACACCGCAGGGATTCGCCCCCGCAAAGCTTATCGAAGCCCACCGCCT
CGCCGGTGAAGAGCAATGGTACGCCACCGATGACGCCGCCCTCGTGGAGCGCTATTTCCCGCAGCAAGCGATTCGCATCT
ACGAAACCGGCTACCACAACATCAAGATCACCACGCCCGAAGATGTCTTCATTGGTGAAGCGATTCTTGCCGGGCTAAAA
GCCAGAAAATCGAAAAATTAA

Upstream 100 bases:

>100_bases
TTGCCGAGCACCGCCTGCTCATGGAAGCCTACAAAACCGCGCTCAAGAACAACTACCGTTTCCTCGCCTACGGGGATGCC
ATGCTTATCGTCTAACGCCC

Downstream 100 bases:

>100_bases
TTCATTTCGCTTCTTGTTTTTAGAAAGGCGACGCCGTATATTAATGGCCCTTAAGGTGAGGTAGCTCAGTTGGTTAGAGC
ACAGGATTCATAACCCTGAG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKTVVIIAASGVGKRMKLDGGRSKQMLEIGGQPVIWHTMKAFQEASTVESVYIATLPDSIPVFKEIAKANGFTKITAIIE
GGKERQDSIGNCMKLIEQEIENSGVMPDAILVHDGARPFIQPEEIDDIARLSATHGACVPATKPKDTIKYVGCNPEIFGE
TLDRSRLLQVQTPQGFAPAKLIEAHRLAGEEQWYATDDAALVERYFPQQAIRIYETGYHNIKITTPEDVFIGEAILAGLK
ARKSKN

Sequences:

>Translated_246_residues
MKTVVIIAASGVGKRMKLDGGRSKQMLEIGGQPVIWHTMKAFQEASTVESVYIATLPDSIPVFKEIAKANGFTKITAIIE
GGKERQDSIGNCMKLIEQEIENSGVMPDAILVHDGARPFIQPEEIDDIARLSATHGACVPATKPKDTIKYVGCNPEIFGE
TLDRSRLLQVQTPQGFAPAKLIEAHRLAGEEQWYATDDAALVERYFPQQAIRIYETGYHNIKITTPEDVFIGEAILAGLK
ARKSKN
>Mature_246_residues
MKTVVIIAASGVGKRMKLDGGRSKQMLEIGGQPVIWHTMKAFQEASTVESVYIATLPDSIPVFKEIAKANGFTKITAIIE
GGKERQDSIGNCMKLIEQEIENSGVMPDAILVHDGARPFIQPEEIDDIARLSATHGACVPATKPKDTIKYVGCNPEIFGE
TLDRSRLLQVQTPQGFAPAKLIEAHRLAGEEQWYATDDAALVERYFPQQAIRIYETGYHNIKITTPEDVFIGEAILAGLK
ARKSKN

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=24.0343347639485, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789104, Length=245, Percent_Identity=31.8367346938775, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CHLTE (Q8KCU3)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662205.1
- ProteinModelPortal:   Q8KCU3
- SMR:   Q8KCU3
- GeneID:   1006683
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1317
- NMPDR:   fig|194439.1.peg.1299
- TIGR:   CT1317
- HOGENOM:   HBG672839
- OMA:   KQMLEIG
- ProtClustDB:   CLSK637702
- BioCyc:   CTEP194439:CT_1317-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 27022; Mature: 27022

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVVIIAASGVGKRMKLDGGRSKQMLEIGGQPVIWHTMKAFQEASTVESVYIATLPDSI
CCEEEEEEECCCCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEECCCCC
PVFKEIAKANGFTKITAIIEGGKERQDSIGNCMKLIEQEIENSGVMPDAILVHDGARPFI
HHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
QPEEIDDIARLSATHGACVPATKPKDTIKYVGCNPEIFGETLDRSRLLQVQTPQGFAPAK
CCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHH
LIEAHRLAGEEQWYATDDAALVERYFPQQAIRIYETGYHNIKITTPEDVFIGEAILAGLK
HHHHHHHCCCCCEECCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
ARKSKN
HHCCCC
>Mature Secondary Structure
MKTVVIIAASGVGKRMKLDGGRSKQMLEIGGQPVIWHTMKAFQEASTVESVYIATLPDSI
CCEEEEEEECCCCCEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEECCCCC
PVFKEIAKANGFTKITAIIEGGKERQDSIGNCMKLIEQEIENSGVMPDAILVHDGARPFI
HHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
QPEEIDDIARLSATHGACVPATKPKDTIKYVGCNPEIFGETLDRSRLLQVQTPQGFAPAK
CCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHH
LIEAHRLAGEEQWYATDDAALVERYFPQQAIRIYETGYHNIKITTPEDVFIGEAILAGLK
HHHHHHHCCCCCEECCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
ARKSKN
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901