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The map label for this gene is rfaD [H]

Identifier: 21674081

GI number: 21674081

Start: 1181941

End: 1182939

Strand: Direct

Name: rfaD [H]

Synonym: CT1258

Alternate gene names: 21674081

Gene position: 1181941-1182939 (Clockwise)

Preceding gene: 21674080

Following gene: 21674082

Centisome position: 54.85

GC content: 56.86

Gene sequence:

>999_bases
ATGATTATCATCACCGGCGGCGCGGGGTTCATCGGCAGCGCCATGCTCTGGGAACTCAACCGCAACGGCACCGATGAGGT
ACTCATCGTCGATGACCTCGGAAGAGCGTCCGAGGGGAGATGGCTCAACCTCCGGGGCCTACGCTACACCGATTTCATTC
ACAAGGACGACCTGCCCGACCTGCTCGAACACGACCGCCTGCCCAAAATCGACGCCGTGATTCACATGGGAGCCATCAGC
TCCACCACCGAACAGGACGCCAACCTTCTGCTGCGCAACAACTACGAATACTCGAAGATGCTCGCCTCGTGGTGCGCAAA
AAAGGGCGTGCGTTTTATCTATGCGTCAAGCGCGGCAACCTTCGGCGACGGCTCCGAAGGATACAGTGACGGCATCGAGG
TGCTCGACCGGCTAAGACCACTGAACATGTACGGCTACTCGAAGCATCTGTTCGACTGCTGGGCGCTGCGCAACGGCATC
CTTGAAAAAGCGGCGGGGCTCAAGTTCTTCAACGTCTACGGGCCCAACGAATACCACAAGGAGGACATGACCAGCGTCGT
CTTCAAGGCGTTCCACCAGATCGGCGACAATGGCAAGGTAAGGCTTTTCAGATCGCACAATCCACAGTACGCCGATGGCG
AGCAGCTGCGCGACTTCGTCTATGTGAAGGATTGCACGAAAATCATGCAATGGCTGCTCGAAACGCCCTCGGCGACGGGA
CTGTTCAACATCGGCACCGGGCAGGCGCGCAGCTTCAGGGATTTGGTCATAGCAACCTTCACGGCGATGGATCGCCCCGT
CAGCATCGAGTTTATCGATATGCCCGAAACGATTCGGGACAAATACCAGTATTATACCTGTGCGGACTCTGCCCATCTCA
GGCAGGCTGGCTACACCGGCCAGATGACGCCGCTCGAAGAGGGAGTGCGAGATTATGTGCAGAACTATCTGAGCAAACCC
TCGCCGCATCTTGATACATTGGCTTTTGAACGGCAATAA

Upstream 100 bases:

>100_bases
ACTCCTCGGTAAAGGGCCAGCTCGTTTACGGGCTGGCCTTTTGCTTTTCCGGCGAGACGGACGTATATTTTTTCGCCTGT
CAACCATTTGACCTTTTTGC

Downstream 100 bases:

>100_bases
CAAACCGGGAAAAAACTTTTGACTGAAAAAACGATCGAATTCGAAGGGATCGAACCCGTCATCATTTTCGGGCCATACGA
CAGCTATCTAAAAAAAGTTC

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MIIITGGAGFIGSAMLWELNRNGTDEVLIVDDLGRASEGRWLNLRGLRYTDFIHKDDLPDLLEHDRLPKIDAVIHMGAIS
STTEQDANLLLRNNYEYSKMLASWCAKKGVRFIYASSAATFGDGSEGYSDGIEVLDRLRPLNMYGYSKHLFDCWALRNGI
LEKAAGLKFFNVYGPNEYHKEDMTSVVFKAFHQIGDNGKVRLFRSHNPQYADGEQLRDFVYVKDCTKIMQWLLETPSATG
LFNIGTGQARSFRDLVIATFTAMDRPVSIEFIDMPETIRDKYQYYTCADSAHLRQAGYTGQMTPLEEGVRDYVQNYLSKP
SPHLDTLAFERQ

Sequences:

>Translated_332_residues
MIIITGGAGFIGSAMLWELNRNGTDEVLIVDDLGRASEGRWLNLRGLRYTDFIHKDDLPDLLEHDRLPKIDAVIHMGAIS
STTEQDANLLLRNNYEYSKMLASWCAKKGVRFIYASSAATFGDGSEGYSDGIEVLDRLRPLNMYGYSKHLFDCWALRNGI
LEKAAGLKFFNVYGPNEYHKEDMTSVVFKAFHQIGDNGKVRLFRSHNPQYADGEQLRDFVYVKDCTKIMQWLLETPSATG
LFNIGTGQARSFRDLVIATFTAMDRPVSIEFIDMPETIRDKYQYYTCADSAHLRQAGYTGQMTPLEEGVRDYVQNYLSKP
SPHLDTLAFERQ
>Mature_332_residues
MIIITGGAGFIGSAMLWELNRNGTDEVLIVDDLGRASEGRWLNLRGLRYTDFIHKDDLPDLLEHDRLPKIDAVIHMGAIS
STTEQDANLLLRNNYEYSKMLASWCAKKGVRFIYASSAATFGDGSEGYSDGIEVLDRLRPLNMYGYSKHLFDCWALRNGI
LEKAAGLKFFNVYGPNEYHKEDMTSVVFKAFHQIGDNGKVRLFRSHNPQYADGEQLRDFVYVKDCTKIMQWLLETPSATG
LFNIGTGQARSFRDLVIATFTAMDRPVSIEFIDMPETIRDKYQYYTCADSAHLRQAGYTGQMTPLEEGVRDYVQNYLSKP
SPHLDTLAFERQ

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790049, Length=319, Percent_Identity=40.7523510971787, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.20 [H]

Molecular weight: Translated: 37723; Mature: 37723

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIITGGAGFIGSAMLWELNRNGTDEVLIVDDLGRASEGRWLNLRGLRYTDFIHKDDLPD
CEEEECCCCHHCCCEEEEECCCCCCCEEEEECCCCCCCCCEEEECCCCHHHCCCCCCCHH
LLEHDRLPKIDAVIHMGAISSTTEQDANLLLRNNYEYSKMLASWCAKKGVRFIYASSAAT
HHHHCCCCCHHHHEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCC
FGDGSEGYSDGIEVLDRLRPLNMYGYSKHLFDCWALRNGILEKAAGLKFFNVYGPNEYHK
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
EDMTSVVFKAFHQIGDNGKVRLFRSHNPQYADGEQLRDFVYVKDCTKIMQWLLETPSATG
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LFNIGTGQARSFRDLVIATFTAMDRPVSIEFIDMPETIRDKYQYYTCADSAHLRQAGYTG
EEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEEEECCCHHHHHCCCCC
QMTPLEEGVRDYVQNYLSKPSPHLDTLAFERQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIIITGGAGFIGSAMLWELNRNGTDEVLIVDDLGRASEGRWLNLRGLRYTDFIHKDDLPD
CEEEECCCCHHCCCEEEEECCCCCCCEEEEECCCCCCCCCEEEECCCCHHHCCCCCCCHH
LLEHDRLPKIDAVIHMGAISSTTEQDANLLLRNNYEYSKMLASWCAKKGVRFIYASSAAT
HHHHCCCCCHHHHEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCC
FGDGSEGYSDGIEVLDRLRPLNMYGYSKHLFDCWALRNGILEKAAGLKFFNVYGPNEYHK
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
EDMTSVVFKAFHQIGDNGKVRLFRSHNPQYADGEQLRDFVYVKDCTKIMQWLLETPSATG
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LFNIGTGQARSFRDLVIATFTAMDRPVSIEFIDMPETIRDKYQYYTCADSAHLRQAGYTG
EEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHEEEECCCHHHHHCCCCC
QMTPLEEGVRDYVQNYLSKPSPHLDTLAFERQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA