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The map label for this gene is lipA [H]

Identifier: 21673904

GI number: 21673904

Start: 1018835

End: 1019707

Strand: Reverse

Name: lipA [H]

Synonym: CT1078

Alternate gene names: 21673904

Gene position: 1019707-1018835 (Counterclockwise)

Preceding gene: 21673905

Following gene: 21673903

Centisome position: 47.32

GC content: 62.54

Gene sequence:

>873_bases
ATGAACTCTGGTCCCGGAAAGAAACCCGACTGGCTCAAGATCAAGCTCGCTTCGGGCTCGTCGTTTGCTTCGACGCGCAA
GCTGCTGAACCGGCACAGCCTCCACACGGTGTGCCGCTCGGCGATGTGCCCGAACCTGCACGAGTGCTGGTCGAAAGGCA
CGGCCACCTTCCTCTTGCTCGGCAACGTCTGCACCCGCTCCTGCCGTTTTTGCGCGGTCGGAACGGAATGCCGGCCGGCT
ATGCCCGATCCGGAGGAGCCGTCGAAGATCGCCGAGGCGGTGAAAACGATGAAGCTCCGCCACGCCGTGCTAACCAGCGT
CAACCGTGACGATCTCGCCGATGGCGGTGCAACGCACTGGGTTGAAACCATCCGCGCGATTCGCGAAGTGAACCCCGGCG
TGAGTCTCGAATGTCTCATTCCCGACTTCAGCGGCAATGAACAGTCGCTCGATTTGGTGATGCAGGAGCTCCCGGAGGTG
CTGAATCACAACATCGAAACCGTGCCGTCACGCTACGCCGCCGTGCGCCCGCAAGCCTTGTACGAACGCTCGCTTGCGGT
GATCGAGCGGGCGAAGCGCCAGTTCCGGCTCGCCACCAAGTCCGGCATGATGGTCGGCATGGGTGAAACGGAGGAGGAGC
TCGAAGCGTCGCTGCACGACCTGCGAGGTCACGGCTGTGACATGGTGACTATCGGCCAGTACCTGCAACCAACCGCCGCG
CATCTGCCGGTGAGCCGCTACGTGACGCCCGAAGAGTTCGAGCGCTACCGCGAAATCGCGCTCGACGCCGGATTCCGGCA
CGTTCAGTCGGGCCCCTTCGTGCGCAGTTCGTACCACGCCGAGGCATTCGAGCCAGTTGAAAAAATTTCCTGA

Upstream 100 bases:

>100_bases
TCCACTCCGGCCACGTCTTCCATCTGGCGATAGCAGGCATAATTGTCAGGATTGGCTATATTCGTGAAGTCTTTTTCAAC
TTCAGTTCTGATACCGCTGC

Downstream 100 bases:

>100_bases
ACCAAAAACCTGACCTGACATGCACACCATTCCGGCGCCTTTTCAGATTTATGCTTACTGGGCCATCGCCCTCATCATCG
GGCTTGCCTTCTTTCGGAAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNSGPGKKPDWLKIKLASGSSFASTRKLLNRHSLHTVCRSAMCPNLHECWSKGTATFLLLGNVCTRSCRFCAVGTECRPA
MPDPEEPSKIAEAVKTMKLRHAVLTSVNRDDLADGGATHWVETIRAIREVNPGVSLECLIPDFSGNEQSLDLVMQELPEV
LNHNIETVPSRYAAVRPQALYERSLAVIERAKRQFRLATKSGMMVGMGETEEELEASLHDLRGHGCDMVTIGQYLQPTAA
HLPVSRYVTPEEFERYREIALDAGFRHVQSGPFVRSSYHAEAFEPVEKIS

Sequences:

>Translated_290_residues
MNSGPGKKPDWLKIKLASGSSFASTRKLLNRHSLHTVCRSAMCPNLHECWSKGTATFLLLGNVCTRSCRFCAVGTECRPA
MPDPEEPSKIAEAVKTMKLRHAVLTSVNRDDLADGGATHWVETIRAIREVNPGVSLECLIPDFSGNEQSLDLVMQELPEV
LNHNIETVPSRYAAVRPQALYERSLAVIERAKRQFRLATKSGMMVGMGETEEELEASLHDLRGHGCDMVTIGQYLQPTAA
HLPVSRYVTPEEFERYREIALDAGFRHVQSGPFVRSSYHAEAFEPVEKIS
>Mature_290_residues
MNSGPGKKPDWLKIKLASGSSFASTRKLLNRHSLHTVCRSAMCPNLHECWSKGTATFLLLGNVCTRSCRFCAVGTECRPA
MPDPEEPSKIAEAVKTMKLRHAVLTSVNRDDLADGGATHWVETIRAIREVNPGVSLECLIPDFSGNEQSLDLVMQELPEV
LNHNIETVPSRYAAVRPQALYERSLAVIERAKRQFRLATKSGMMVGMGETEEELEASLHDLRGHGCDMVTIGQYLQPTAA
HLPVSRYVTPEEFERYREIALDAGFRHVQSGPFVRSSYHAEAFEPVEKIS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=42.1985815602837, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=41.8032786885246, Blast_Score=204, Evalue=6e-53,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=50.3597122302158, Blast_Score=290, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=255, Percent_Identity=45.4901960784314, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=285, Percent_Identity=47.0175438596491, Blast_Score=261, Evalue=8e-71,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=45.7446808510638, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 32248; Mature: 32248

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSGPGKKPDWLKIKLASGSSFASTRKLLNRHSLHTVCRSAMCPNLHECWSKGTATFLLL
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEH
GNVCTRSCRFCAVGTECRPAMPDPEEPSKIAEAVKTMKLRHAVLTSVNRDDLADGGATHW
HHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHH
VETIRAIREVNPGVSLECLIPDFSGNEQSLDLVMQELPEVLNHNIETVPSRYAAVRPQAL
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
YERSLAVIERAKRQFRLATKSGMMVGMGETEEELEASLHDLRGHGCDMVTIGQYLQPTAA
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCEEEHHHHHCCHHH
HLPVSRYVTPEEFERYREIALDAGFRHVQSGPFVRSSYHAEAFEPVEKIS
HCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNSGPGKKPDWLKIKLASGSSFASTRKLLNRHSLHTVCRSAMCPNLHECWSKGTATFLLL
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEH
GNVCTRSCRFCAVGTECRPAMPDPEEPSKIAEAVKTMKLRHAVLTSVNRDDLADGGATHW
HHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHH
VETIRAIREVNPGVSLECLIPDFSGNEQSLDLVMQELPEVLNHNIETVPSRYAAVRPQAL
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
YERSLAVIERAKRQFRLATKSGMMVGMGETEEELEASLHDLRGHGCDMVTIGQYLQPTAA
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCEEEHHHHHCCHHH
HLPVSRYVTPEEFERYREIALDAGFRHVQSGPFVRSSYHAEAFEPVEKIS
HCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA