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The map label for this gene is fumA [H]

Identifier: 21673662

GI number: 21673662

Start: 793250

End: 794761

Strand: Direct

Name: fumA [H]

Synonym: CT0833

Alternate gene names: 21673662

Gene position: 793250-794761 (Clockwise)

Preceding gene: 21673661

Following gene: 21673663

Centisome position: 36.81

GC content: 61.71

Gene sequence:

>1512_bases
ATGATCGCCTTGATCACCGAGACCTCCACGAATCTGCCGAGCGACGTTCGCAAGGCCATCGCCGATGCCGTTGGCAATGA
TGCTGCCGATTCGAGGGCCGGACTTGCCATGTCTGCCATCACGCTCAATATCGACATGGCGGTCGATAATGTCGGCCCGG
TTTGCCAGGATACCGGTATGCCTACCTTCTTCGTGCACACGCCGAAAGGCGCTGACCAGCTCGCCATGAAGCGCGACATC
GAGGAGGCTGTCGTCGAGGCAACCCGAACCGGCAAGCTTCGTCCCAATGCGGTCGATTCGCTGACCGGTAAGAACTCCGG
CAACAACCTTGGCTGCCATGTGCCGGTGATCCATTTCGAGCCGTGGGATCGCGACGAGATCGAGGTGAAGCTCATCCTCA
AAGGCGGCGGCTGCGAGAACAAAAACATCCAGTACTCGCTGCCTGCCGATATTCCCGGACTTGGCCGCGCGGCTCGCGAC
CTCGACGGCGTGCGTAAGTGCATCCTCCACGCCGTGTACCAGGCGCAGGGACAGGGGTGCAGCCCCGGCTTCATCGGCGT
CGGCGTCGGTGGCGACCGCACCAGCAGCTTCGAGCTGGCCAAGAAGCAGTTGCTCCGCTCGGTCGATGACACCAATTCCG
ATGCGGTGCTGGCCGAACTCGAACAGGAGATTCTCGACAAGGCTAACCGCCTGAACATCGGGCCGATGGGCTTTGCCGGC
AAGACCACGCTGCTTGGCTGCAAGATCGGCACATCGCATCGCGTTCCGGCTAGCTTCTTTGTCTCGGTAGCCTACAATTG
CTGGGCCTATCGCCGTCTTGGCGTCATCATCGACCCGAAAGAGGGTTCGATTTCCGAGTGGCAGTACCGCTATCCGGGCG
AAATCAAGCGCATGGCGCGCGGCGCGGGCATTCCGCTGACGGGCCGTGAGGTGGTGCTCACCGCGCCGGTCAGCGAGGAG
ACGATCCGCTCGCTCAAGGTTGGCGACATCGTGATCGTTGATGGCGAAATGCACACCGGACGCGATGCCTTCCACCACTA
CATCATGCACCATGATCTGCCCGAGGGTCTCGACATCCGCGGCGGCATCATCTACCACTGCGGCCCGGTGATGCTCAAGA
ACGAGGCGGGCGAGTACACGGTCGTTGCTGCCGGGCCGACCACTTCGATTCGCGAGGAGCCTTACCAGTCGGACGTGATC
GAAAAGCTCGGACTTCGCGCGGTGATTGGCAAGGGCGGCATGGGGCCGAAGACGCTTGCGGGCTTGCAGAAGCACGGTGC
GGTCTATCTCAACGCCATCGGCGGTGCGGCGCAGTACTACGCCAGGTGCATCGAAAAGGTGACCGGCGTCGATTTCTTGG
AAGAGATGGGCGTGCCGGAGGCGATGTGGCACTTGCAGGCCAAAGCCTTCCCGTGCATCGTCACGATGGACGCGCACGGC
AACAGCCTGCACAAGCAGGTCGATGAGGAGTCCTTCGCGATGCTTGAAAACATCGGCAAGGAGCAGGCGTAA

Upstream 100 bases:

>100_bases
TCGTCCTGCGGGATGGCAACGACAAGGCTGATTGCAGCAGACGCGTTCACAATCTTCGTTCACTCCAAGCTTGCCGACTA
TGAAACAGTTCAGGGATTCA

Downstream 100 bases:

>100_bases
GTCTCAACCGGAAAACAGTTTACTTTCTATGCCGCGCCGCGTCAGCCGCAACAGCCTGCCGCGGCGCGGCCATAATGGAT
CAGTTAAAACCAGCGAGAGC

Product: fumarate hydratase, class I, aerobic

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MIALITETSTNLPSDVRKAIADAVGNDAADSRAGLAMSAITLNIDMAVDNVGPVCQDTGMPTFFVHTPKGADQLAMKRDI
EEAVVEATRTGKLRPNAVDSLTGKNSGNNLGCHVPVIHFEPWDRDEIEVKLILKGGGCENKNIQYSLPADIPGLGRAARD
LDGVRKCILHAVYQAQGQGCSPGFIGVGVGGDRTSSFELAKKQLLRSVDDTNSDAVLAELEQEILDKANRLNIGPMGFAG
KTTLLGCKIGTSHRVPASFFVSVAYNCWAYRRLGVIIDPKEGSISEWQYRYPGEIKRMARGAGIPLTGREVVLTAPVSEE
TIRSLKVGDIVIVDGEMHTGRDAFHHYIMHHDLPEGLDIRGGIIYHCGPVMLKNEAGEYTVVAAGPTTSIREEPYQSDVI
EKLGLRAVIGKGGMGPKTLAGLQKHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHLQAKAFPCIVTMDAHG
NSLHKQVDEESFAMLENIGKEQA

Sequences:

>Translated_503_residues
MIALITETSTNLPSDVRKAIADAVGNDAADSRAGLAMSAITLNIDMAVDNVGPVCQDTGMPTFFVHTPKGADQLAMKRDI
EEAVVEATRTGKLRPNAVDSLTGKNSGNNLGCHVPVIHFEPWDRDEIEVKLILKGGGCENKNIQYSLPADIPGLGRAARD
LDGVRKCILHAVYQAQGQGCSPGFIGVGVGGDRTSSFELAKKQLLRSVDDTNSDAVLAELEQEILDKANRLNIGPMGFAG
KTTLLGCKIGTSHRVPASFFVSVAYNCWAYRRLGVIIDPKEGSISEWQYRYPGEIKRMARGAGIPLTGREVVLTAPVSEE
TIRSLKVGDIVIVDGEMHTGRDAFHHYIMHHDLPEGLDIRGGIIYHCGPVMLKNEAGEYTVVAAGPTTSIREEPYQSDVI
EKLGLRAVIGKGGMGPKTLAGLQKHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHLQAKAFPCIVTMDAHG
NSLHKQVDEESFAMLENIGKEQA
>Mature_503_residues
MIALITETSTNLPSDVRKAIADAVGNDAADSRAGLAMSAITLNIDMAVDNVGPVCQDTGMPTFFVHTPKGADQLAMKRDI
EEAVVEATRTGKLRPNAVDSLTGKNSGNNLGCHVPVIHFEPWDRDEIEVKLILKGGGCENKNIQYSLPADIPGLGRAARD
LDGVRKCILHAVYQAQGQGCSPGFIGVGVGGDRTSSFELAKKQLLRSVDDTNSDAVLAELEQEILDKANRLNIGPMGFAG
KTTLLGCKIGTSHRVPASFFVSVAYNCWAYRRLGVIIDPKEGSISEWQYRYPGEIKRMARGAGIPLTGREVVLTAPVSEE
TIRSLKVGDIVIVDGEMHTGRDAFHHYIMHHDLPEGLDIRGGIIYHCGPVMLKNEAGEYTVVAAGPTTSIREEPYQSDVI
EKLGLRAVIGKGGMGPKTLAGLQKHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHLQAKAFPCIVTMDAHG
NSLHKQVDEESFAMLENIGKEQA

Specific function: Unknown

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1789443, Length=173, Percent_Identity=37.5722543352601, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789442, Length=271, Percent_Identity=30.6273062730627, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787897, Length=495, Percent_Identity=24.8484848484848, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790564, Length=479, Percent_Identity=24.8434237995825, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54188; Mature: 54188

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALITETSTNLPSDVRKAIADAVGNDAADSRAGLAMSAITLNIDMAVDNVGPVCQDTGM
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEECCCCCCHHCCCC
PTFFVHTPKGADQLAMKRDIEEAVVEATRTGKLRPNAVDSLTGKNSGNNLGCHVPVIHFE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEC
PWDRDEIEVKLILKGGGCENKNIQYSLPADIPGLGRAARDLDGVRKCILHAVYQAQGQGC
CCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SPGFIGVGVGGDRTSSFELAKKQLLRSVDDTNSDAVLAELEQEILDKANRLNIGPMGFAG
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KTTLLGCKIGTSHRVPASFFVSVAYNCWAYRRLGVIIDPKEGSISEWQYRYPGEIKRMAR
CCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECEEECCCCCCCCCCEEECCHHHHHHHC
GAGIPLTGREVVLTAPVSEETIRSLKVGDIVIVDGEMHTGRDAFHHYIMHHDLPEGLDIR
CCCCCCCCCCEEEECCCCHHHHHHEECCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
GGIIYHCGPVMLKNEAGEYTVVAAGPTTSIREEPYQSDVIEKLGLRAVIGKGGMGPKTLA
CCEEEECCCEEEECCCCCEEEEEECCCCCHHCCCCHHHHHHHCCCEEEECCCCCCHHHHH
GLQKHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHLQAKAFPCIVTMDAHG
HHHHCCCEEEEECCHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCC
NSLHKQVDEESFAMLENIGKEQA
CHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIALITETSTNLPSDVRKAIADAVGNDAADSRAGLAMSAITLNIDMAVDNVGPVCQDTGM
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEECCCCCCHHCCCC
PTFFVHTPKGADQLAMKRDIEEAVVEATRTGKLRPNAVDSLTGKNSGNNLGCHVPVIHFE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEEEEEEC
PWDRDEIEVKLILKGGGCENKNIQYSLPADIPGLGRAARDLDGVRKCILHAVYQAQGQGC
CCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SPGFIGVGVGGDRTSSFELAKKQLLRSVDDTNSDAVLAELEQEILDKANRLNIGPMGFAG
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KTTLLGCKIGTSHRVPASFFVSVAYNCWAYRRLGVIIDPKEGSISEWQYRYPGEIKRMAR
CCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECEEECCCCCCCCCCEEECCHHHHHHHC
GAGIPLTGREVVLTAPVSEETIRSLKVGDIVIVDGEMHTGRDAFHHYIMHHDLPEGLDIR
CCCCCCCCCCEEEECCCCHHHHHHEECCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
GGIIYHCGPVMLKNEAGEYTVVAAGPTTSIREEPYQSDVIEKLGLRAVIGKGGMGPKTLA
CCEEEECCCEEEECCCCCEEEEEECCCCCHHCCCCHHHHHHHCCCEEEECCCCCCHHHHH
GLQKHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHLQAKAFPCIVTMDAHG
HHHHCCCEEEEECCHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCC
NSLHKQVDEESFAMLENIGKEQA
CHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8473853 [H]