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The map label for this gene is ygcM [C]

Identifier: 21673612

GI number: 21673612

Start: 754128

End: 754577

Strand: Reverse

Name: ygcM [C]

Synonym: CT0782

Alternate gene names: 21673612

Gene position: 754577-754128 (Counterclockwise)

Preceding gene: 21673613

Following gene: 21673611

Centisome position: 35.02

GC content: 47.11

Gene sequence:

>450_bases
ATGAACGACATCGTCGAAAAGCCGAGAAAAATTTACGTTACACGCCAAATCGAGTTCAATGCCGCCCACCGTCTGTTCAA
TCCGGAGCTATCGGACGAGGAAAACCAGCAGCTCTATGGAAAATGTTCTGGTAAGTATGGACATGGGCACAACTATCTGC
TTGAAATCACCCTATCGGGCATCATTGACCGGAAAACCGGTTATTTGTTCGATCTCAAGGAGCTAAAGAAAATTCTTGAA
GAGGAGATTGTGGCACGGTTCGACCACCGGCATCTGAACCATGAGGTCAACGAACTTGCAGGCCACGTCCCGACAACAGA
GATTCTTGCCGTCATCGTCTGGGAGATTCTCGATTCCCGGCTGAAAACCATTACCAAACAGGAGGTTAGCCTCCATGAAG
TCATAATACATGAAACAGGAAAAAACAGTGTCACCTACCGTGGAGAATAA

Upstream 100 bases:

>100_bases
CTTATAACTGGCAGGCCGGGTTAAACTGAAAAATCACATCGAATCCCCGTCATCCGGCAGATGCTGGAAGACAGTCAATG
AAACACCAGCGATACCCTTG

Downstream 100 bases:

>100_bases
CCGATCTGCAGAATCACGACTTTCGCAATGCGATCTCGATGAGTGCTTCGACGAGTCGCATGATCGTGACGAGGAGGTGC
TCGGGTCGATGACCGACGCC

Product: 6-pyruvoyl tetrahydrobiopterin synthase, putative

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MNDIVEKPRKIYVTRQIEFNAAHRLFNPELSDEENQQLYGKCSGKYGHGHNYLLEITLSGIIDRKTGYLFDLKELKKILE
EEIVARFDHRHLNHEVNELAGHVPTTEILAVIVWEILDSRLKTITKQEVSLHEVIIHETGKNSVTYRGE

Sequences:

>Translated_149_residues
MNDIVEKPRKIYVTRQIEFNAAHRLFNPELSDEENQQLYGKCSGKYGHGHNYLLEITLSGIIDRKTGYLFDLKELKKILE
EEIVARFDHRHLNHEVNELAGHVPTTEILAVIVWEILDSRLKTITKQEVSLHEVIIHETGKNSVTYRGE
>Mature_149_residues
MNDIVEKPRKIYVTRQIEFNAAHRLFNPELSDEENQQLYGKCSGKYGHGHNYLLEITLSGIIDRKTGYLFDLKELKKILE
EEIVARFDHRHLNHEVNELAGHVPTTEILAVIVWEILDSRLKTITKQEVSLHEVIIHETGKNSVTYRGE

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=137, Percent_Identity=43.7956204379562, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI115532226, Length=138, Percent_Identity=37.6811594202899, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI115532224, Length=138, Percent_Identity=37.6811594202899, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19550074, Length=137, Percent_Identity=47.4452554744526, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24585378, Length=137, Percent_Identity=47.4452554744526, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 17319; Mature: 17319

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIVEKPRKIYVTRQIEFNAAHRLFNPELSDEENQQLYGKCSGKYGHGHNYLLEITLSG
CCCHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECH
IIDRKTGYLFDLKELKKILEEEIVARFDHRHLNHEVNELAGHVPTTEILAVIVWEILDSR
HHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LKTITKQEVSLHEVIIHETGKNSVTYRGE
HHHHHHHHHHHHHHHEECCCCCCEEECCC
>Mature Secondary Structure
MNDIVEKPRKIYVTRQIEFNAAHRLFNPELSDEENQQLYGKCSGKYGHGHNYLLEITLSG
CCCHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEECH
IIDRKTGYLFDLKELKKILEEEIVARFDHRHLNHEVNELAGHVPTTEILAVIVWEILDSR
HHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LKTITKQEVSLHEVIIHETGKNSVTYRGE
HHHHHHHHHHHHHHHEECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA