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The map label for this gene is folE

Identifier: 21673611

GI number: 21673611

Start: 753494

End: 754168

Strand: Reverse

Name: folE

Synonym: CT0781

Alternate gene names: 21673611

Gene position: 754168-753494 (Counterclockwise)

Preceding gene: 21673612

Following gene: 21673590

Centisome position: 35.0

GC content: 56.74

Gene sequence:

>675_bases
ATGAAACAGGAAAAAACAGTGTCACCTACCGTGGAGAATAACCGATCTGCAGAATCACGACTTTCGCAATGCGATCTCGA
TGAGTGCTTCGACGAGTCGCATGATCGTGACGAGGAGGTGCTCGGGTCGATGACCGACGCCGTATACAGCTTGCTGAAAG
GGGTTGGCGAGGATCCGGAGCGCGAAGGATTGCTCCTGACCCCCGAGCGGGTGGCCAAATCCCTGCGATTCCTGACGAAG
GGATACCGGCAGGATCCAGAGCAGTTGCTCAAAAAAGCGGTGTTCACAGAGTCGTACGACGAGATGGTGCTGGTGAAGGA
CATCGATATTTACTCGATGTGCGAACACCACATGCTCCCCTTCTTCGGCAAGGCGCACGTGGCCTACATTCCCGATGGCA
AGATCGTGGGCTTATCGAAAATTCCAAGAGTCGTCGAGGTGTTCGCGCGGCGCTTGCAGGTACAGGAGCGGCTAACCCAG
CAGATCAGGGACGCCATTCAGAATGTGCTCAACCCGCGCGGTGTGGCCGTGGTAATCGAAGCGACACACATGTGCATGGT
GATGCGCGGCGTGGAGAAGCAGAACGCGGTGACGACAACCTCCGCCATGTCCGGAGATTTCATGACCAGCCAGTCTACCC
GAAGCGAGTTCCTGCGCCTGATCGGCAACCACTGA

Upstream 100 bases:

>100_bases
CCGACAACAGAGATTCTTGCCGTCATCGTCTGGGAGATTCTCGATTCCCGGCTGAAAACCATTACCAAACAGGAGGTTAG
CCTCCATGAAGTCATAATAC

Downstream 100 bases:

>100_bases
CCACACGTCAGCATTTCTTTACAGCTAAAAGCGATGAGGCATCCGGATATCCCCTGCAACGCAAGGGAAACCGGATGCCT
TTACTTTTACCAGCCACTCT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKQEKTVSPTVENNRSAESRLSQCDLDECFDESHDRDEEVLGSMTDAVYSLLKGVGEDPEREGLLLTPERVAKSLRFLTK
GYRQDPEQLLKKAVFTESYDEMVLVKDIDIYSMCEHHMLPFFGKAHVAYIPDGKIVGLSKIPRVVEVFARRLQVQERLTQ
QIRDAIQNVLNPRGVAVVIEATHMCMVMRGVEKQNAVTTTSAMSGDFMTSQSTRSEFLRLIGNH

Sequences:

>Translated_224_residues
MKQEKTVSPTVENNRSAESRLSQCDLDECFDESHDRDEEVLGSMTDAVYSLLKGVGEDPEREGLLLTPERVAKSLRFLTK
GYRQDPEQLLKKAVFTESYDEMVLVKDIDIYSMCEHHMLPFFGKAHVAYIPDGKIVGLSKIPRVVEVFARRLQVQERLTQ
QIRDAIQNVLNPRGVAVVIEATHMCMVMRGVEKQNAVTTTSAMSGDFMTSQSTRSEFLRLIGNH
>Mature_224_residues
MKQEKTVSPTVENNRSAESRLSQCDLDECFDESHDRDEEVLGSMTDAVYSLLKGVGEDPEREGLLLTPERVAKSLRFLTK
GYRQDPEQLLKKAVFTESYDEMVLVKDIDIYSMCEHHMLPFFGKAHVAYIPDGKIVGLSKIPRVVEVFARRLQVQERLTQ
QIRDAIQNVLNPRGVAVVIEATHMCMVMRGVEKQNAVTTTSAMSGDFMTSQSTRSEFLRLIGNH

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=189, Percent_Identity=53.968253968254, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI4503949, Length=189, Percent_Identity=53.968253968254, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI66932970, Length=159, Percent_Identity=49.685534591195, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI66932972, Length=150, Percent_Identity=52, Blast_Score=174, Evalue=6e-44,
Organism=Escherichia coli, GI1788476, Length=199, Percent_Identity=41.7085427135678, Blast_Score=130, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17560486, Length=185, Percent_Identity=58.3783783783784, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=54.4444444444444, Blast_Score=211, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24656782, Length=184, Percent_Identity=55.4347826086957, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24656791, Length=186, Percent_Identity=54.8387096774194, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24656796, Length=186, Percent_Identity=54.8387096774194, Blast_Score=204, Evalue=3e-53,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_CHLTE (Q8KEA8)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661676.1
- ProteinModelPortal:   Q8KEA8
- SMR:   Q8KEA8
- GeneID:   1006416
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0781
- NMPDR:   fig|194439.1.peg.770
- TIGR:   CT0781
- HOGENOM:   HBG370191
- OMA:   ARIVEMF
- ProtClustDB:   PRK09347
- BioCyc:   CTEP194439:CT_0781-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 25368; Mature: 25368

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEKTVSPTVENNRSAESRLSQCDLDECFDESHDRDEEVLGSMTDAVYSLLKGVGEDPE
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
REGLLLTPERVAKSLRFLTKGYRQDPEQLLKKAVFTESYDEMVLVKDIDIYSMCEHHMLP
CCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCC
FFGKAHVAYIPDGKIVGLSKIPRVVEVFARRLQVQERLTQQIRDAIQNVLNPRGVAVVIE
CCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEH
ATHMCMVMRGVEKQNAVTTTSAMSGDFMTSQSTRSEFLRLIGNH
HHHHHHHHHCCCHHCCEEHHHHCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQEKTVSPTVENNRSAESRLSQCDLDECFDESHDRDEEVLGSMTDAVYSLLKGVGEDPE
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
REGLLLTPERVAKSLRFLTKGYRQDPEQLLKKAVFTESYDEMVLVKDIDIYSMCEHHMLP
CCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCC
FFGKAHVAYIPDGKIVGLSKIPRVVEVFARRLQVQERLTQQIRDAIQNVLNPRGVAVVIE
CCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEH
ATHMCMVMRGVEKQNAVTTTSAMSGDFMTSQSTRSEFLRLIGNH
HHHHHHHHHCCCHHCCEEHHHHCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901