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The map label for this gene is hupL [H]

Identifier: 21673607

GI number: 21673607

Start: 750686

End: 752119

Strand: Direct

Name: hupL [H]

Synonym: CT0777

Alternate gene names: 21673607

Gene position: 750686-752119 (Clockwise)

Preceding gene: 21673606

Following gene: 21673608

Centisome position: 34.84

GC content: 56.14

Gene sequence:

>1434_bases
ATGAACGCCACGCAGCAGACGCAGGACCATCTGGTGCACTTTTATTACCTGCATGCCCTTGACTGGGTTGATGTGGTCAG
CGCATTGAAGGCTGATCCCGGCCAGGCTTCTGCGATCGCGCAGAGCATTTCGGCTTGGCCGAAGTCCTCGGTCGGCTACT
TCAAGGATTTGCAGAAGCGCCTCGTGGCTTTTGTCGAAAGCGGTCAGCTCGGCATCTTTTCGAACGGCTACTGGGGCCAT
CCTGCCTACAAATTGCCGCCGGAGGTGAACCTCATCGCCGTGGCGCACTACCTCGAAGCGCTCGATTTCCAGAAAGAGAT
CGTCAAGATCCAGACCGTCTTTGATGGCAAGAATCCGCATCCGAACTATGTCGTTGGCGGTATGGCCTGCGCGATCGATC
CGAACAGCGATACGGCGATCAACATCGAGCGTCTCAACATGATCAAGAAGATCATCGACGAGACGCAGACCTTTATCGAT
CAGGTTTACATCCCTGACCTCATCGCCATCGCCGGATTCTACAAGGAGTGGCTTTATGGTGGAGGTCTTGGCAACTTTAT
GAGCTACGGCGACTTTCCGGAGACCACGCTCGACGATTACGAAAAGCTTTTGTGGCCGCGCGGCATCATCATGGGCAAGG
ATCTTACGACAGTGCTCGACGTCGATCCACGTGATATGTCTCAGATCAAGGAGGAGATCGCCCACAGCTGGTACACCTAC
AGCAATGGCAGCGATCAGGGTTTACACCCGTGGGAAGGGGAGACCAACCCTAAATACACCGCTCCGAAGCCACCGTTTGA
GTATCTCGATACGGACAAGAAGTACAGTTGGCTCAAAACGCCGCGCTGGAAAGACCAGCCGGTAGAGGTCGGTCCGCTGG
CCAGCGTGCTGGTAGCCTACGCGAAGGGTGATTCGATGATCAAGGATACGGTCGGTATGGTGCTTTCGAAGCTTGAAGTC
GGCCCCGAAGCACTCTATTCGACCCTCGGACGAACAGCCGCGCGCGGCATCGAGTGCAAGCAGACCGCCGGCTTCATGCG
CCACTTCTACGACGAGCTGGTTGCCAACGTTAAAACCGGTGATTACCAGACCTTCAACAGCGAACGTTGGGAGCCCTTGA
CCTGGCCAAAAGAGTGCAAAGGCTTTGGCTATACCGAAGCGCCGCGCGGCTCGCTCGGTCACTGGATCAGGATCGAAAAC
GGCAAGATCGGCGACTATCAGATCGTGGTGCCATCGACCTGGAACGCTTCACCGAGGGATGGCCGTGGCAACTCAGGCGC
GTACGAAGCTGCCTTGAAGGGCACGCCGATGCACGATCCCTCGCAGCCGCTTGAGATCCTTCGCACGGTGCACTCGTTCG
ATCCTTGTCTTGCCTGTGCGTCGCACGTGTTTGACATGAATGGTAACGAGATTACGAAGGTAAGGATCGTTTAA

Upstream 100 bases:

>100_bases
ATTTTAATAATAACAATTGTAAATCATATTTACCTGTTATCTGGCGACGTTCATCCGGTACAGGAGGAAAGATGCAGGCG
AAATCCTCATCAGGAATCTG

Downstream 100 bases:

>100_bases
TACGGAGGTTATCATGGGACGGATTATTGAGGAAATCTATGTGTGGCGCCTGCCGGTCAGGCTCTACCACTGGGTCAACG
CCTTCAGCATCGCGGTACTG

Product: uptake hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNATQQTQDHLVHFYYLHALDWVDVVSALKADPGQASAIAQSISAWPKSSVGYFKDLQKRLVAFVESGQLGIFSNGYWGH
PAYKLPPEVNLIAVAHYLEALDFQKEIVKIQTVFDGKNPHPNYVVGGMACAIDPNSDTAINIERLNMIKKIIDETQTFID
QVYIPDLIAIAGFYKEWLYGGGLGNFMSYGDFPETTLDDYEKLLWPRGIIMGKDLTTVLDVDPRDMSQIKEEIAHSWYTY
SNGSDQGLHPWEGETNPKYTAPKPPFEYLDTDKKYSWLKTPRWKDQPVEVGPLASVLVAYAKGDSMIKDTVGMVLSKLEV
GPEALYSTLGRTAARGIECKQTAGFMRHFYDELVANVKTGDYQTFNSERWEPLTWPKECKGFGYTEAPRGSLGHWIRIEN
GKIGDYQIVVPSTWNASPRDGRGNSGAYEAALKGTPMHDPSQPLEILRTVHSFDPCLACASHVFDMNGNEITKVRIV

Sequences:

>Translated_477_residues
MNATQQTQDHLVHFYYLHALDWVDVVSALKADPGQASAIAQSISAWPKSSVGYFKDLQKRLVAFVESGQLGIFSNGYWGH
PAYKLPPEVNLIAVAHYLEALDFQKEIVKIQTVFDGKNPHPNYVVGGMACAIDPNSDTAINIERLNMIKKIIDETQTFID
QVYIPDLIAIAGFYKEWLYGGGLGNFMSYGDFPETTLDDYEKLLWPRGIIMGKDLTTVLDVDPRDMSQIKEEIAHSWYTY
SNGSDQGLHPWEGETNPKYTAPKPPFEYLDTDKKYSWLKTPRWKDQPVEVGPLASVLVAYAKGDSMIKDTVGMVLSKLEV
GPEALYSTLGRTAARGIECKQTAGFMRHFYDELVANVKTGDYQTFNSERWEPLTWPKECKGFGYTEAPRGSLGHWIRIEN
GKIGDYQIVVPSTWNASPRDGRGNSGAYEAALKGTPMHDPSQPLEILRTVHSFDPCLACASHVFDMNGNEITKVRIV
>Mature_477_residues
MNATQQTQDHLVHFYYLHALDWVDVVSALKADPGQASAIAQSISAWPKSSVGYFKDLQKRLVAFVESGQLGIFSNGYWGH
PAYKLPPEVNLIAVAHYLEALDFQKEIVKIQTVFDGKNPHPNYVVGGMACAIDPNSDTAINIERLNMIKKIIDETQTFID
QVYIPDLIAIAGFYKEWLYGGGLGNFMSYGDFPETTLDDYEKLLWPRGIIMGKDLTTVLDVDPRDMSQIKEEIAHSWYTY
SNGSDQGLHPWEGETNPKYTAPKPPFEYLDTDKKYSWLKTPRWKDQPVEVGPLASVLVAYAKGDSMIKDTVGMVLSKLEV
GPEALYSTLGRTAARGIECKQTAGFMRHFYDELVANVKTGDYQTFNSERWEPLTWPKECKGFGYTEAPRGSLGHWIRIEN
GKIGDYQIVVPSTWNASPRDGRGNSGAYEAALKGTPMHDPSQPLEILRTVHSFDPCLACASHVFDMNGNEITKVRIV

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=489, Percent_Identity=53.1697341513292, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1789368, Length=489, Percent_Identity=43.3537832310838, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 53452; Mature: 53452

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATQQTQDHLVHFYYLHALDWVDVVSALKADPGQASAIAQSISAWPKSSVGYFKDLQKR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
LVAFVESGQLGIFSNGYWGHPAYKLPPEVNLIAVAHYLEALDFQKEIVKIQTVFDGKNPH
HHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHEECCCCCC
PNYVVGGMACAIDPNSDTAINIERLNMIKKIIDETQTFIDQVYIPDLIAIAGFYKEWLYG
CCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGLGNFMSYGDFPETTLDDYEKLLWPRGIIMGKDLTTVLDVDPRDMSQIKEEIAHSWYTY
CCHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCEEE
SNGSDQGLHPWEGETNPKYTAPKPPFEYLDTDKKYSWLKTPRWKDQPVEVGPLASVLVAY
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AKGDSMIKDTVGMVLSKLEVGPEALYSTLGRTAARGIECKQTAGFMRHFYDELVANVKTG
HCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
DYQTFNSERWEPLTWPKECKGFGYTEAPRGSLGHWIRIENGKIGDYQIVVPSTWNASPRD
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
GRGNSGAYEAALKGTPMHDPSQPLEILRTVHSFDPCLACASHVFDMNGNEITKVRIV
CCCCCCCCHHEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MNATQQTQDHLVHFYYLHALDWVDVVSALKADPGQASAIAQSISAWPKSSVGYFKDLQKR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
LVAFVESGQLGIFSNGYWGHPAYKLPPEVNLIAVAHYLEALDFQKEIVKIQTVFDGKNPH
HHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHEECCCCCC
PNYVVGGMACAIDPNSDTAINIERLNMIKKIIDETQTFIDQVYIPDLIAIAGFYKEWLYG
CCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGLGNFMSYGDFPETTLDDYEKLLWPRGIIMGKDLTTVLDVDPRDMSQIKEEIAHSWYTY
CCHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHCEEE
SNGSDQGLHPWEGETNPKYTAPKPPFEYLDTDKKYSWLKTPRWKDQPVEVGPLASVLVAY
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AKGDSMIKDTVGMVLSKLEVGPEALYSTLGRTAARGIECKQTAGFMRHFYDELVANVKTG
HCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
DYQTFNSERWEPLTWPKECKGFGYTEAPRGSLGHWIRIENGKIGDYQIVVPSTWNASPRD
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
GRGNSGAYEAALKGTPMHDPSQPLEILRTVHSFDPCLACASHVFDMNGNEITKVRIV
CCCCCCCCHHEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2402452; 2103457 [H]