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The map label for this gene is ndhF [H]

Identifier: 21673604

GI number: 21673604

Start: 744945

End: 747212

Strand: Direct

Name: ndhF [H]

Synonym: CT0774

Alternate gene names: 21673604

Gene position: 744945-747212 (Clockwise)

Preceding gene: 21673603

Following gene: 21673605

Centisome position: 34.57

GC content: 55.78

Gene sequence:

>2268_bases
ATGATGCACAGTTTAATCCAGTTATCCATCGCTGTCCTGCTACTGCCGCTGCTCTCGTTTGTGGTGCTGATATTTTTCAA
CCGCAGGCTCCCGCGTGGTGGCGATTTCGTTGGCGTCGGTCTGCTTGGCACGACGCTGGCTATAGCGCTGTATATCTTCT
GGACGGTGATCGTTCAGCACTACGATCCGGCCTTCAGGCTTGCGTGGGATTTCACCTGGCTTGATTTTGGCAATGTCCCC
GGCGTCGGACCGCTGCAGGTCAAGATGGGTATCGTGATTGACAACCTGGCGGCGATCATGCTGGCAATGGTTTCGCTCAT
CAGTTTCCTGGTGCACCTCTACTCGACCGGGTATATGAAGGGGGAGATGTATTACGGACGCTTTTTTGCCTATCTCGGCA
TCTTTACCTTCTCGATGTTCGGCATCGTGCTTTCTGACAACCTCTTTTCGATCTACATCTTCTGGGAGCTTGTCGGTCTT
TCGTCTTACCTTCTGATCGGTTTCTACTTCCACAAAGACAGCGCCGCCGACGCGCAGAAAAAGGCCTTTCTCACCAACCG
TGTTGGCGACATCGGTATGTGGCTCGGCATCCTGATTCTCTATTCCCAGTTTCACACCTTCGGCTACCAGGAGATTTTCA
ACCATATCAAGAATGGCGACTTCCACATGTCGCAGGCTTGGCTGACCGCCGCCGGCATTCTGCTCTTCATGGGCTGCGTC
GGTAAATCGGCCCAGTTCCCGCTGCATGTCTGGCTTCCTGACGCCATGGAAGGCCCGACTCCGGTTTCGGCGCTCATCCA
CGCGGCAACGATGGTTGCGGCTGGCGTCTATTTTGTGGCCCGTATCTTTGTGCTTCTGACGCCGGATGCGCTGCACGTGA
TCGCTTTCATCGGCGCATTCACCGCTTTCATGGCGGCAACCATCGCCATCACGCAGTTCGACATCAAGCGCGTGCTGGCC
TATTCGACCGTTTCACAGCTTGGTTACATGGTGCTCGGTCTCGGTGTCGGCTCCTACTCCGCCGCGCTGTTCCATCTCCT
GACCCACGCTTTCTTCAAAGCCTGCCTCTTCCTCGGTTCCGGCGCGATCATCCACGCGATGCATCACGAGCAGGACATGC
GCTGGATGGGAGGCCTGTACAAAAAGATGCCCTGGACGTTTGTCACTTTCAGCATCGCCACATTGGCGCTTGCCGGTCTG
CCGCTAACCAGCGGCTTCCTTTCGAAAGATGCGATTCTGGCCGGTTCGCTCGGCTTCGCGCAGGCCGAGGGCGGCGGCAT
CTACTATCTCGTGCCAGTGTTTGGCTTCGGTGCCGCAGTGCTGACCGCCTTCTATATGGGCCGTCAGATATGGATGGTCT
TCTTCGGCGAGAACCGTACACATCTGAAACCGAAATCGGCGCACAGCATGGATGATCATGATGACGGCGATGTCGATCAC
ATTCATGACGCTGCGCACGGTGGTCACGATCATCATCCGGTGCACGAGGTTGCCTGGAACATGAGGCTTCCGCTGGTTGT
TCTTGCCACGCTCTCTGTCTTTATCGTCTTTTCGCCTGATCCGCTTGATGGCGGCAAGGGCTGGTTCATGCACCTGGTGC
AGACTCCGGCCACGGTTGTCAGCGTTGCCGAGATGGCGCACGAAGGCGCGCAACTTCACGCTCCGGAAGCTGGCAAGTTG
CTTGCTGAGGCGCATACGGATACCCAGCATGTCGAAGCCGCCACATCGCGTGAGGCTGAAGGGCAGCATGGTCCTGTTTT
CGCTGATCCCCGTCAGGCTGAGATTGCTCACATGACGCACGCCAATCACTACACGGCGATCAAGCTGTCGTCGATCATGG
TGGTTATCGGTATCGGTATGGCGCTCATTGTCTATGTTTTCAGGATTATCGATCCCGACAAGACGGCGCAGGCGATTCGT
CCGCTTTACCTCTACTCGTTCAACAAGTGGTACTGGGACGAGATTTACGACGCAACCATCATCAAGGGCTCCATACTGAT
CTCGAAGATTCTTGCCTGGTTCGACACGAACATCGTTGACGGTCTTGTCAACGGCGTGGCGACGATCTTCAGGAAGTTCG
CCTTCTTCAATGGCGGCGTTGACAAATATGTAGTTGACGGACTGGTCAACTTCACGGCCTTCACCGTCCAGACGACCGGC
GCGGTCTTCAGAAAGATTCAGACGGGCAAGGTGCAGACCTATCTGGTCATGGTGGTTTTTGCGGTTCTGGGCTGGTTCGC
CCTTTACTTTGCGCACCTCGTCAAATAA

Upstream 100 bases:

>100_bases
AAGACCTTCAAGACGGTCGATGTATCCAGCGTGGACACCATGAAGGAGTGATCCGCCCCGGTAAAACACTTATAGCTGCA
AATTCTTTGTAAAACGAAAC

Downstream 100 bases:

>100_bases
CCGAACGATTTAATTTTTACTCTCCGAACATAACAGGTATTGAACATGCTGAGCTTCATTGTATTTCTGCCGATCATTGC
CGGACTTGTCATTTTGGCTG

Product: NADH dehydrogenase I, subunit 5

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MMHSLIQLSIAVLLLPLLSFVVLIFFNRRLPRGGDFVGVGLLGTTLAIALYIFWTVIVQHYDPAFRLAWDFTWLDFGNVP
GVGPLQVKMGIVIDNLAAIMLAMVSLISFLVHLYSTGYMKGEMYYGRFFAYLGIFTFSMFGIVLSDNLFSIYIFWELVGL
SSYLLIGFYFHKDSAADAQKKAFLTNRVGDIGMWLGILILYSQFHTFGYQEIFNHIKNGDFHMSQAWLTAAGILLFMGCV
GKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYFVARIFVLLTPDALHVIAFIGAFTAFMAATIAITQFDIKRVLA
YSTVSQLGYMVLGLGVGSYSAALFHLLTHAFFKACLFLGSGAIIHAMHHEQDMRWMGGLYKKMPWTFVTFSIATLALAGL
PLTSGFLSKDAILAGSLGFAQAEGGGIYYLVPVFGFGAAVLTAFYMGRQIWMVFFGENRTHLKPKSAHSMDDHDDGDVDH
IHDAAHGGHDHHPVHEVAWNMRLPLVVLATLSVFIVFSPDPLDGGKGWFMHLVQTPATVVSVAEMAHEGAQLHAPEAGKL
LAEAHTDTQHVEAATSREAEGQHGPVFADPRQAEIAHMTHANHYTAIKLSSIMVVIGIGMALIVYVFRIIDPDKTAQAIR
PLYLYSFNKWYWDEIYDATIIKGSILISKILAWFDTNIVDGLVNGVATIFRKFAFFNGGVDKYVVDGLVNFTAFTVQTTG
AVFRKIQTGKVQTYLVMVVFAVLGWFALYFAHLVK

Sequences:

>Translated_755_residues
MMHSLIQLSIAVLLLPLLSFVVLIFFNRRLPRGGDFVGVGLLGTTLAIALYIFWTVIVQHYDPAFRLAWDFTWLDFGNVP
GVGPLQVKMGIVIDNLAAIMLAMVSLISFLVHLYSTGYMKGEMYYGRFFAYLGIFTFSMFGIVLSDNLFSIYIFWELVGL
SSYLLIGFYFHKDSAADAQKKAFLTNRVGDIGMWLGILILYSQFHTFGYQEIFNHIKNGDFHMSQAWLTAAGILLFMGCV
GKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYFVARIFVLLTPDALHVIAFIGAFTAFMAATIAITQFDIKRVLA
YSTVSQLGYMVLGLGVGSYSAALFHLLTHAFFKACLFLGSGAIIHAMHHEQDMRWMGGLYKKMPWTFVTFSIATLALAGL
PLTSGFLSKDAILAGSLGFAQAEGGGIYYLVPVFGFGAAVLTAFYMGRQIWMVFFGENRTHLKPKSAHSMDDHDDGDVDH
IHDAAHGGHDHHPVHEVAWNMRLPLVVLATLSVFIVFSPDPLDGGKGWFMHLVQTPATVVSVAEMAHEGAQLHAPEAGKL
LAEAHTDTQHVEAATSREAEGQHGPVFADPRQAEIAHMTHANHYTAIKLSSIMVVIGIGMALIVYVFRIIDPDKTAQAIR
PLYLYSFNKWYWDEIYDATIIKGSILISKILAWFDTNIVDGLVNGVATIFRKFAFFNGGVDKYVVDGLVNFTAFTVQTTG
AVFRKIQTGKVQTYLVMVVFAVLGWFALYFAHLVK
>Mature_755_residues
MMHSLIQLSIAVLLLPLLSFVVLIFFNRRLPRGGDFVGVGLLGTTLAIALYIFWTVIVQHYDPAFRLAWDFTWLDFGNVP
GVGPLQVKMGIVIDNLAAIMLAMVSLISFLVHLYSTGYMKGEMYYGRFFAYLGIFTFSMFGIVLSDNLFSIYIFWELVGL
SSYLLIGFYFHKDSAADAQKKAFLTNRVGDIGMWLGILILYSQFHTFGYQEIFNHIKNGDFHMSQAWLTAAGILLFMGCV
GKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYFVARIFVLLTPDALHVIAFIGAFTAFMAATIAITQFDIKRVLA
YSTVSQLGYMVLGLGVGSYSAALFHLLTHAFFKACLFLGSGAIIHAMHHEQDMRWMGGLYKKMPWTFVTFSIATLALAGL
PLTSGFLSKDAILAGSLGFAQAEGGGIYYLVPVFGFGAAVLTAFYMGRQIWMVFFGENRTHLKPKSAHSMDDHDDGDVDH
IHDAAHGGHDHHPVHEVAWNMRLPLVVLATLSVFIVFSPDPLDGGKGWFMHLVQTPATVVSVAEMAHEGAQLHAPEAGKL
LAEAHTDTQHVEAATSREAEGQHGPVFADPRQAEIAHMTHANHYTAIKLSSIMVVIGIGMALIVYVFRIIDPDKTAQAIR
PLYLYSFNKWYWDEIYDATIIKGSILISKILAWFDTNIVDGLVNGVATIFRKFAFFNGGVDKYVVDGLVNFTAFTVQTTG
AVFRKIQTGKVQTYLVMVVFAVLGWFALYFAHLVK

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=408, Percent_Identity=35.5392156862745, Blast_Score=235, Evalue=1e-61,
Organism=Escherichia coli, GI1788614, Length=456, Percent_Identity=44.5175438596491, Blast_Score=339, Evalue=4e-94,
Organism=Escherichia coli, GI1788829, Length=430, Percent_Identity=31.8604651162791, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788831, Length=339, Percent_Identity=30.9734513274336, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI1788827, Length=342, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1788613, Length=415, Percent_Identity=27.710843373494, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI145693160, Length=301, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI2367154, Length=182, Percent_Identity=30.2197802197802, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 83441; Mature: 83441

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHSLIQLSIAVLLLPLLSFVVLIFFNRRLPRGGDFVGVGLLGTTLAIALYIFWTVIVQH
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
YDPAFRLAWDFTWLDFGNVPGVGPLQVKMGIVIDNLAAIMLAMVSLISFLVHLYSTGYMK
CCCCEEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GEMYYGRFFAYLGIFTFSMFGIVLSDNLFSIYIFWELVGLSSYLLIGFYFHKDSAADAQK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHEEHEEEEEECCCCCHHHH
KAFLTNRVGDIGMWLGILILYSQFHTFGYQEIFNHIKNGDFHMSQAWLTAAGILLFMGCV
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYFVARIFVLLTPDALHVIAFIGAF
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TAFMAATIAITQFDIKRVLAYSTVSQLGYMVLGLGVGSYSAALFHLLTHAFFKACLFLGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
GAIIHAMHHEQDMRWMGGLYKKMPWTFVTFSIATLALAGLPLTSGFLSKDAILAGSLGFA
CCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCC
QAEGGGIYYLVPVFGFGAAVLTAFYMGRQIWMVFFGENRTHLKPKSAHSMDDHDDGDVDH
EECCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
IHDAAHGGHDHHPVHEVAWNMRLPLVVLATLSVFIVFSPDPLDGGKGWFMHLVQTPATVV
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCHHHHH
SVAEMAHEGAQLHAPEAGKLLAEAHTDTQHVEAATSREAEGQHGPVFADPRQAEIAHMTH
HHHHHHHCCCEECCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHC
ANHYTAIKLSSIMVVIGIGMALIVYVFRIIDPDKTAQAIRPLYLYSFNKWYWDEIYDATI
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHEEECCCHHHHHHHHHHH
IKGSILISKILAWFDTNIVDGLVNGVATIFRKFAFFNGGVDKYVVDGLVNFTAFTVQTTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEHHH
AVFRKIQTGKVQTYLVMVVFAVLGWFALYFAHLVK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMHSLIQLSIAVLLLPLLSFVVLIFFNRRLPRGGDFVGVGLLGTTLAIALYIFWTVIVQH
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
YDPAFRLAWDFTWLDFGNVPGVGPLQVKMGIVIDNLAAIMLAMVSLISFLVHLYSTGYMK
CCCCEEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GEMYYGRFFAYLGIFTFSMFGIVLSDNLFSIYIFWELVGLSSYLLIGFYFHKDSAADAQK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHEEHEEEEEECCCCCHHHH
KAFLTNRVGDIGMWLGILILYSQFHTFGYQEIFNHIKNGDFHMSQAWLTAAGILLFMGCV
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYFVARIFVLLTPDALHVIAFIGAF
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TAFMAATIAITQFDIKRVLAYSTVSQLGYMVLGLGVGSYSAALFHLLTHAFFKACLFLGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
GAIIHAMHHEQDMRWMGGLYKKMPWTFVTFSIATLALAGLPLTSGFLSKDAILAGSLGFA
CCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCC
QAEGGGIYYLVPVFGFGAAVLTAFYMGRQIWMVFFGENRTHLKPKSAHSMDDHDDGDVDH
EECCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
IHDAAHGGHDHHPVHEVAWNMRLPLVVLATLSVFIVFSPDPLDGGKGWFMHLVQTPATVV
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCHHHHH
SVAEMAHEGAQLHAPEAGKLLAEAHTDTQHVEAATSREAEGQHGPVFADPRQAEIAHMTH
HHHHHHHCCCEECCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHC
ANHYTAIKLSSIMVVIGIGMALIVYVFRIIDPDKTAQAIRPLYLYSFNKWYWDEIYDATI
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHEEECCCHHHHHHHHHHH
IKGSILISKILAWFDTNIVDGLVNGVATIFRKFAFFNGGVDKYVVDGLVNFTAFTVQTTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEHHH
AVFRKIQTGKVQTYLVMVVFAVLGWFALYFAHLVK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]