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The map label for this gene is thiF [H]

Identifier: 21673526

GI number: 21673526

Start: 682110

End: 682853

Strand: Reverse

Name: thiF [H]

Synonym: CT0696

Alternate gene names: 21673526

Gene position: 682853-682110 (Counterclockwise)

Preceding gene: 21673527

Following gene: 21673525

Centisome position: 31.69

GC content: 59.81

Gene sequence:

>744_bases
GTGCACGTGAGCCTCAGCGACGAGCAGTGCCAGCGCTACGCCCGCCACCTCGCGCTGCCGGAAGTTGGCGAGGCGGGGCA
AGAGAAGCTGCTGCACTCGAAAGTGCTGGTCATCGGTGCAGGCGGCCTCGGCTCCCCTGCTGCGTTCTACCTTGCCGCAG
CAGGTGTCGGCACCATTGGCCTCATGGACGGCGACACGGTCGATCTCTCGAACCTGCAACGGCAGATTCTGCACACGACC
GCCTCGGTCGGTGCGAATAAAACCGCCTCGGCGCAGGAGCGTCTCAAAGCGCTCGACCCGTCGATCCGCATCGAAACTCA
CCCCTTCCGGCTGAGAAAAGAGAACGCCACGGAAATTCTCGCCAGATACGACTTCGTCATCGACGCCACGGACAACTTCG
CATCCCGCTTCCTCATCGCTCGCGCCTGCCACGAAGCATCGAAACCATGGTCTCACGGCGGAATCCGTAACTTCCACGGC
CAGACCATGACCATCATCCCCGGGCAAACAGCCTGCTACTGCTGCATCTTCCACGAAGAGGACGAATCAAAAGAGGCTAT
TCCACAAGGCCCTATCGGCGCACTGCCGGGCGTCATCGGATCGATTCAAGCCATCGAAGCGATCAAATATTTGCTCAACA
TCGGCACACTGCTGACCGACGCGCTGATGACGTTCGATGCGCTCACGATGAGTTTCCGCAAAGTCGCGGTACGGAGGAAT
TCAAGATGTGCGTTGTGTGGATGA

Upstream 100 bases:

>100_bases
GAAGCGTCGAAGCGTTTTGCGCCGCATTGCGCGCAAAAGATCTGGAACCAGTGTTCAAAAACTGGGACGCGGCCTACAAC
AACCCCCTGCCCGCAGAGGA

Downstream 100 bases:

>100_bases
AAAACTGCTGCCTGAGAGCACTGCGGTGAGCATCCCCTGATTTTCGCACGAGTTGAACGCGGCATCGACTCGTTGACGAT
ATTCGGGATTAATGCTACTT

Product: thiamine biosynthesis protein ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MHVSLSDEQCQRYARHLALPEVGEAGQEKLLHSKVLVIGAGGLGSPAAFYLAAAGVGTIGLMDGDTVDLSNLQRQILHTT
ASVGANKTASAQERLKALDPSIRIETHPFRLRKENATEILARYDFVIDATDNFASRFLIARACHEASKPWSHGGIRNFHG
QTMTIIPGQTACYCCIFHEEDESKEAIPQGPIGALPGVIGSIQAIEAIKYLLNIGTLLTDALMTFDALTMSFRKVAVRRN
SRCALCG

Sequences:

>Translated_247_residues
MHVSLSDEQCQRYARHLALPEVGEAGQEKLLHSKVLVIGAGGLGSPAAFYLAAAGVGTIGLMDGDTVDLSNLQRQILHTT
ASVGANKTASAQERLKALDPSIRIETHPFRLRKENATEILARYDFVIDATDNFASRFLIARACHEASKPWSHGGIRNFHG
QTMTIIPGQTACYCCIFHEEDESKEAIPQGPIGALPGVIGSIQAIEAIKYLLNIGTLLTDALMTFDALTMSFRKVAVRRN
SRCALCG
>Mature_247_residues
MHVSLSDEQCQRYARHLALPEVGEAGQEKLLHSKVLVIGAGGLGSPAAFYLAAAGVGTIGLMDGDTVDLSNLQRQILHTT
ASVGANKTASAQERLKALDPSIRIETHPFRLRKENATEILARYDFVIDATDNFASRFLIARACHEASKPWSHGGIRNFHG
QTMTIIPGQTACYCCIFHEEDESKEAIPQGPIGALPGVIGSIQAIEAIKYLLNIGTLLTDALMTFDALTMSFRKVAVRRN
SRCALCG

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=248, Percent_Identity=37.9032258064516, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI13376212, Length=254, Percent_Identity=27.1653543307087, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI38327032, Length=215, Percent_Identity=25.5813953488372, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4885649, Length=143, Percent_Identity=35.6643356643357, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI23510338, Length=208, Percent_Identity=27.4038461538462, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI23510340, Length=208, Percent_Identity=27.4038461538462, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI38045944, Length=185, Percent_Identity=31.3513513513514, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI38045942, Length=164, Percent_Identity=32.9268292682927, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI87082356, Length=245, Percent_Identity=42.0408163265306, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787048, Length=246, Percent_Identity=39.8373983739837, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789177, Length=150, Percent_Identity=30.6666666666667, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=248, Percent_Identity=34.2741935483871, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI193203301, Length=150, Percent_Identity=38, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17508819, Length=255, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71984476, Length=166, Percent_Identity=30.1204819277108, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI86565433, Length=206, Percent_Identity=28.6407766990291, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=206, Percent_Identity=28.6407766990291, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=253, Percent_Identity=37.5494071146245, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6320598, Length=185, Percent_Identity=28.6486486486486, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322639, Length=199, Percent_Identity=29.1457286432161, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325323, Length=190, Percent_Identity=26.8421052631579, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=40.6374501992032, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24660640, Length=204, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641311, Length=254, Percent_Identity=30.7086614173228, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20129973, Length=155, Percent_Identity=31.6129032258064, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28573937, Length=196, Percent_Identity=27.0408163265306, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 26704; Mature: 26704

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVSLSDEQCQRYARHLALPEVGEAGQEKLLHSKVLVIGAGGLGSPAAFYLAAAGVGTIG
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCEEE
LMDGDTVDLSNLQRQILHTTASVGANKTASAQERLKALDPSIRIETHPFRLRKENATEIL
ECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEECCCHHHHHH
ARYDFVIDATDNFASRFLIARACHEASKPWSHGGIRNFHGQTMTIIPGQTACYCCIFHEE
HHHHEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECC
DESKEAIPQGPIGALPGVIGSIQAIEAIKYLLNIGTLLTDALMTFDALTMSFRKVAVRRN
CCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRCALCG
CCCCCCC
>Mature Secondary Structure
MHVSLSDEQCQRYARHLALPEVGEAGQEKLLHSKVLVIGAGGLGSPAAFYLAAAGVGTIG
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCEEE
LMDGDTVDLSNLQRQILHTTASVGANKTASAQERLKALDPSIRIETHPFRLRKENATEIL
ECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEECCCHHHHHH
ARYDFVIDATDNFASRFLIARACHEASKPWSHGGIRNFHGQTMTIIPGQTACYCCIFHEE
HHHHEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECC
DESKEAIPQGPIGALPGVIGSIQAIEAIKYLLNIGTLLTDALMTFDALTMSFRKVAVRRN
CCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRCALCG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]