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The map label for this gene is thiH [H]

Identifier: 21673527

GI number: 21673527

Start: 682844

End: 683911

Strand: Reverse

Name: thiH [H]

Synonym: CT0697

Alternate gene names: 21673527

Gene position: 683911-682844 (Counterclockwise)

Preceding gene: 21673528

Following gene: 21673526

Centisome position: 31.74

GC content: 62.73

Gene sequence:

>1068_bases
ATGATTGCGCTGCCCGCATGGCTGACCGACGAGCGGCTGTCGGAAGATATCGAACCGCTGTTGCGACAAACGGATAACGA
GTCGCTCGAACGGCTTGCCGCCGAAGCGCAGGCAGTGACACTGCGCCGTTTCGGGCGCGTCATTTCGCTCTATACGCCGC
TCTACCTCTCCAACTTCTGCTCGAGCGGTTGCGTCTATTGCGGCTTCGCTTCGGACAGACGTTCGCCGCGCCGCAAGCTG
GATACTGACGAAATCGAAAAGGAGCTGCTCGCAATGAAGGCTCTCGGCGTCAGCGACGTTTTGCTGCTCACCGGCGAGCG
CACCAACTCGGTGGGATTCGACTATCTGCGTCGCGCCGTGGATATCGCCGCCCGCCACATGCCGCGCGTAGCCGTCGAGG
CGTTTCCGATGAGCGTCGCAGAGTATCGCGGCCTGGCCGAATGTGGGTGCACCGGCCTGACGATTTACCAGGAAACCTAC
GATCCGGATCATTACCGCGAGCTGCACCGCTGGGGGCCGAAGCAGGATTTCCTCGAACGGCTCGAAACGCCGGAACGCGC
CATCACCGGCGGCATCCGGAGCGTCGGCATCGGCGCACTGCTCGGCCTGTCGGAGCCGGTCGGCGAAGCGCTCGCCGTGT
TGCGCCACGCGCGGTATCTGTGCAAAACGTACTGGAAAGCAGGCGTCACGGTCTCCTTTCCCCGCATCCGCCCGCAGGAG
GGCGGCTTTCAGCCCAGCTTCACGGTCTCGGATCGCTTCCTCGCACGAATGATCTTCGCCTTCCGCATCGGAATGCCGGA
TGTCGATCTGGTGCTCTCGACGCGAGAGAGTTCGAATTTTCGGGACGGCATGGCTGGCCTCGGCATCACCCGCATGAGCA
TCGCCAGCCGCACCACCGTTGGCGGCTACGTCGAAAAGGAGACGGCTGGAGCCAGCCAGTTCGAGGTGAGCGACAACCGA
AGCGTCGAAGCGTTTTGCGCCGCATTGCGCGCAAAAGATCTGGAACCAGTGTTCAAAAACTGGGACGCGGCCTACAACAA
CCCCCTGCCCGCAGAGGAGTGCACGTGA

Upstream 100 bases:

>100_bases
CGGTCGAGGCGGGATTCGAGGCACGGAACGCAGGGCTGATGCCACGTTCGGGATCGGCGGTCGCAACCAGCCCGCTCACC
TCGTTCCTGGGAGCTACTCG

Downstream 100 bases:

>100_bases
GCCTCAGCGACGAGCAGTGCCAGCGCTACGCCCGCCACCTCGCGCTGCCGGAAGTTGGCGAGGCGGGGCAAGAGAAGCTG
CTGCACTCGAAAGTGCTGGT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIALPAWLTDERLSEDIEPLLRQTDNESLERLAAEAQAVTLRRFGRVISLYTPLYLSNFCSSGCVYCGFASDRRSPRRKL
DTDEIEKELLAMKALGVSDVLLLTGERTNSVGFDYLRRAVDIAARHMPRVAVEAFPMSVAEYRGLAECGCTGLTIYQETY
DPDHYRELHRWGPKQDFLERLETPERAITGGIRSVGIGALLGLSEPVGEALAVLRHARYLCKTYWKAGVTVSFPRIRPQE
GGFQPSFTVSDRFLARMIFAFRIGMPDVDLVLSTRESSNFRDGMAGLGITRMSIASRTTVGGYVEKETAGASQFEVSDNR
SVEAFCAALRAKDLEPVFKNWDAAYNNPLPAEECT

Sequences:

>Translated_355_residues
MIALPAWLTDERLSEDIEPLLRQTDNESLERLAAEAQAVTLRRFGRVISLYTPLYLSNFCSSGCVYCGFASDRRSPRRKL
DTDEIEKELLAMKALGVSDVLLLTGERTNSVGFDYLRRAVDIAARHMPRVAVEAFPMSVAEYRGLAECGCTGLTIYQETY
DPDHYRELHRWGPKQDFLERLETPERAITGGIRSVGIGALLGLSEPVGEALAVLRHARYLCKTYWKAGVTVSFPRIRPQE
GGFQPSFTVSDRFLARMIFAFRIGMPDVDLVLSTRESSNFRDGMAGLGITRMSIASRTTVGGYVEKETAGASQFEVSDNR
SVEAFCAALRAKDLEPVFKNWDAAYNNPLPAEECT
>Mature_355_residues
MIALPAWLTDERLSEDIEPLLRQTDNESLERLAAEAQAVTLRRFGRVISLYTPLYLSNFCSSGCVYCGFASDRRSPRRKL
DTDEIEKELLAMKALGVSDVLLLTGERTNSVGFDYLRRAVDIAARHMPRVAVEAFPMSVAEYRGLAECGCTGLTIYQETY
DPDHYRELHRWGPKQDFLERLETPERAITGGIRSVGIGALLGLSEPVGEALAVLRHARYLCKTYWKAGVTVSFPRIRPQE
GGFQPSFTVSDRFLARMIFAFRIGMPDVDLVLSTRESSNFRDGMAGLGITRMSIASRTTVGGYVEKETAGASQFEVSDNR
SVEAFCAALRAKDLEPVFKNWDAAYNNPLPAEECT

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=330, Percent_Identity=41.2121212121212, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39591; Mature: 39591

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALPAWLTDERLSEDIEPLLRQTDNESLERLAAEAQAVTLRRFGRVISLYTPLYLSNFC
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGCVYCGFASDRRSPRRKLDTDEIEKELLAMKALGVSDVLLLTGERTNSVGFDYLRRAV
CCCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
DIAARHMPRVAVEAFPMSVAEYRGLAECGCTGLTIYQETYDPDHYRELHRWGPKQDFLER
HHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHH
LETPERAITGGIRSVGIGALLGLSEPVGEALAVLRHARYLCKTYWKAGVTVSFPRIRPQE
HCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GGFQPSFTVSDRFLARMIFAFRIGMPDVDLVLSTRESSNFRDGMAGLGITRMSIASRTTV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCHHHHHHHHCCCC
GGYVEKETAGASQFEVSDNRSVEAFCAALRAKDLEPVFKNWDAAYNNPLPAEECT
CCEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHCCCHHCCCCCCHHCCC
>Mature Secondary Structure
MIALPAWLTDERLSEDIEPLLRQTDNESLERLAAEAQAVTLRRFGRVISLYTPLYLSNFC
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSGCVYCGFASDRRSPRRKLDTDEIEKELLAMKALGVSDVLLLTGERTNSVGFDYLRRAV
CCCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
DIAARHMPRVAVEAFPMSVAEYRGLAECGCTGLTIYQETYDPDHYRELHRWGPKQDFLER
HHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHH
LETPERAITGGIRSVGIGALLGLSEPVGEALAVLRHARYLCKTYWKAGVTVSFPRIRPQE
HCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GGFQPSFTVSDRFLARMIFAFRIGMPDVDLVLSTRESSNFRDGMAGLGITRMSIASRTTV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCHHHHHHHHCCCC
GGYVEKETAGASQFEVSDNRSVEAFCAALRAKDLEPVFKNWDAAYNNPLPAEECT
CCEEECCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHCCCHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]