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The map label for this gene is nudG [H]

Identifier: 21673518

GI number: 21673518

Start: 678791

End: 679225

Strand: Direct

Name: nudG [H]

Synonym: CT0687

Alternate gene names: 21673518

Gene position: 678791-679225 (Clockwise)

Preceding gene: 21673517

Following gene: 21673519

Centisome position: 31.5

GC content: 58.62

Gene sequence:

>435_bases
ATGGTTATCGGCGACGTCGTTTGCGCCATCATCGAGCGTGATGGCCGGTTTCTCATAGCCCGGAGACCGGAGGGGAAACA
TCTTGCACGGAAATGGGAGTTCCCGGGCGGCAAGGTCGAGGCTGGCGAATCGGAGGCAGCAGCGCTTGATCGGGAGCTCC
AGGAAGAGCTTGGAGTGAGGGTGGAAATTATCGAACGCCTCACTCCGGTCGAGCACAGCTACCCGGACCGGTCGCTTCGG
TTGATCGCTTTTCGTTGCAGGATTGTCGATGGAGTGCCGGATGCTGGCGAGCATGAGGAGCTGCGATGGATCGAAATCGA
TGAAGCCGGCGCATACGATTTTCCGGAGGCAGATCTTCCCATTCTTGCCGAGTATCGCCTCAAAATCGCCGCTGTGCCAC
CGGGTGCTCCCGATCAGCTGCATAAAGCGGATTGA

Upstream 100 bases:

>100_bases
GATGGAGAGGTGAGGTTTCGTTGGCCGAACTTTTTCTTCCTTTTTCCCTTCCTTGAGTGCGGTTTGCGAATTGTGCCTTC
CATAACTTGGAACTGAAATA

Downstream 100 bases:

>100_bases
GTACAGCTCTTTTCAGGGAGGGCAAGGTTCATTGTTCCGGCTGAACGGGCGCAGTTCCGTGTCGTGCCTGGTGAACGCTC
GCGTGATTTTTCAGCATTGA

Product: Nudix family protein, MutT subfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MVIGDVVCAIIERDGRFLIARRPEGKHLARKWEFPGGKVEAGESEAAALDRELQEELGVRVEIIERLTPVEHSYPDRSLR
LIAFRCRIVDGVPDAGEHEELRWIEIDEAGAYDFPEADLPILAEYRLKIAAVPPGAPDQLHKAD

Sequences:

>Translated_144_residues
MVIGDVVCAIIERDGRFLIARRPEGKHLARKWEFPGGKVEAGESEAAALDRELQEELGVRVEIIERLTPVEHSYPDRSLR
LIAFRCRIVDGVPDAGEHEELRWIEIDEAGAYDFPEADLPILAEYRLKIAAVPPGAPDQLHKAD
>Mature_144_residues
MVIGDVVCAIIERDGRFLIARRPEGKHLARKWEFPGGKVEAGESEAAALDRELQEELGVRVEIIERLTPVEHSYPDRSLR
LIAFRCRIVDGVPDAGEHEELRWIEIDEAGAYDFPEADLPILAEYRLKIAAVPPGAPDQLHKAD

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=133, Percent_Identity=40.6015037593985, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI1786288, Length=120, Percent_Identity=34.1666666666667, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 16148; Mature: 16148

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIGDVVCAIIERDGRFLIARRPEGKHLARKWEFPGGKVEAGESEAAALDRELQEELGVR
CCHHHEEHEEHHCCCCEEEEECCCCCCHHEECCCCCCEEECCCHHHHHHHHHHHHHHCCE
VEIIERLTPVEHSYPDRSLRLIAFRCRIVDGVPDAGEHEELRWIEIDEAGAYDFPEADLP
EEHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
ILAEYRLKIAAVPPGAPDQLHKAD
EEEECEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MVIGDVVCAIIERDGRFLIARRPEGKHLARKWEFPGGKVEAGESEAAALDRELQEELGVR
CCHHHEEHEEHHCCCCEEEEECCCCCCHHEECCCCCCEEECCCHHHHHHHHHHHHHHCCE
VEIIERLTPVEHSYPDRSLRLIAFRCRIVDGVPDAGEHEELRWIEIDEAGAYDFPEADLP
EEHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
ILAEYRLKIAAVPPGAPDQLHKAD
EEEECEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]