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The map label for this gene is rpoE [H]

Identifier: 21673480

GI number: 21673480

Start: 644239

End: 644835

Strand: Reverse

Name: rpoE [H]

Synonym: CT0648

Alternate gene names: 21673480

Gene position: 644835-644239 (Counterclockwise)

Preceding gene: 21673482

Following gene: 21673479

Centisome position: 29.92

GC content: 46.06

Gene sequence:

>597_bases
ATGAAAGAGAACAAACAGACGCTCACAGCTCAGGAACAGCTCAAACAGCAGGAGTTTCAAAAGGAGGCTGTCGCGCATAT
CAATTCACTCTACAACTATGCGCTGCATCTCACGATGAACCCTGACGATGCCCATGATCTGGTGCAGGAGACATACCTGA
AAGCATATAGATTTTTCGATTCCTTCGAGCGGGGAACAAACTGCAAGGCGTGGTTGTTCAAAATTCTCAAAAACAATTAC
ATCAACAAATTTCGTAAAAACGCAAGGGAACCCGGCAAAGTCGATTACGACCTGATCAAGGACTTTTACCATACGATCAA
GGATGTGCAGAGCGACACAACCGAAGCCGAGTCGGATTTTTTCCATTCCCTGTTGCACGAAGAGGTTTACCAGGCATTGC
AGTCACTTCCCGAAGAGTTCAGGGAAGTCATACAGTTGTGTGACATTGAAGGATTTACCTATGAGGAGATTGCCAACATG
GTCGAAAGCCCGATCGGAACGGTTCGATCACGTTTGTACAGAGGCAGAAAACTGTTGCGTGCCAAACTTGAAGATTACGC
CAAAAAGCATGGATACGACACCGAAATCGATCAGTGA

Upstream 100 bases:

>100_bases
TTTTTATATTGGTTGTTATGAACGTCCTTATCAAAGGTCATCGAATTCGCGGCTCTATTCAGGAGATCTCCTCTCAAAGC
ATTCGGCAAAACCACACCCC

Downstream 100 bases:

>100_bases
TCCCTTTCATTCAAACAATTTATCAGTTGACAGCCATGAACTGTAACAAAGCAATGGTGCTCATGAGCGCAGCTATTGAC
GGGGAACTTTCCGCGAAAGA

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKENKQTLTAQEQLKQQEFQKEAVAHINSLYNYALHLTMNPDDAHDLVQETYLKAYRFFDSFERGTNCKAWLFKILKNNY
INKFRKNAREPGKVDYDLIKDFYHTIKDVQSDTTEAESDFFHSLLHEEVYQALQSLPEEFREVIQLCDIEGFTYEEIANM
VESPIGTVRSRLYRGRKLLRAKLEDYAKKHGYDTEIDQ

Sequences:

>Translated_198_residues
MKENKQTLTAQEQLKQQEFQKEAVAHINSLYNYALHLTMNPDDAHDLVQETYLKAYRFFDSFERGTNCKAWLFKILKNNY
INKFRKNAREPGKVDYDLIKDFYHTIKDVQSDTTEAESDFFHSLLHEEVYQALQSLPEEFREVIQLCDIEGFTYEEIANM
VESPIGTVRSRLYRGRKLLRAKLEDYAKKHGYDTEIDQ
>Mature_198_residues
MKENKQTLTAQEQLKQQEFQKEAVAHINSLYNYALHLTMNPDDAHDLVQETYLKAYRFFDSFERGTNCKAWLFKILKNNY
INKFRKNAREPGKVDYDLIKDFYHTIKDVQSDTTEAESDFFHSLLHEEVYQALQSLPEEFREVIQLCDIEGFTYEEIANM
VESPIGTVRSRLYRGRKLLRAKLEDYAKKHGYDTEIDQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=147, Percent_Identity=32.6530612244898, Blast_Score=97, Evalue=5e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23493; Mature: 23493

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENKQTLTAQEQLKQQEFQKEAVAHINSLYNYALHLTMNPDDAHDLVQETYLKAYRFFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHH
SFERGTNCKAWLFKILKNNYINKFRKNAREPGKVDYDLIKDFYHTIKDVQSDTTEAESDF
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
FHSLLHEEVYQALQSLPEEFREVIQLCDIEGFTYEEIANMVESPIGTVRSRLYRGRKLLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKLEDYAKKHGYDTEIDQ
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKENKQTLTAQEQLKQQEFQKEAVAHINSLYNYALHLTMNPDDAHDLVQETYLKAYRFFD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHH
SFERGTNCKAWLFKILKNNYINKFRKNAREPGKVDYDLIKDFYHTIKDVQSDTTEAESDF
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
FHSLLHEEVYQALQSLPEEFREVIQLCDIEGFTYEEIANMVESPIGTVRSRLYRGRKLLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKLEDYAKKHGYDTEIDQ
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]