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The map label for this gene is ilvB [H]

Identifier: 21673453

GI number: 21673453

Start: 619129

End: 620850

Strand: Reverse

Name: ilvB [H]

Synonym: CT0618

Alternate gene names: 21673453

Gene position: 620850-619129 (Counterclockwise)

Preceding gene: 21673454

Following gene: 21673452

Centisome position: 28.81

GC content: 60.34

Gene sequence:

>1722_bases
ATGCATAACAACGGAGAGAAACTGATCGGATCCGAAATATTTTTCGAGTGTCTAAGGCGTGAAAACGTCGAATACATCTT
CGGCTACCCCGGCGGGGCATTGCTGAAAGTCTATGAAACCCTGCACGATGTCGAGGATATTGAGCACATTCTGGCGCGCC
ACGAACAGGGTGCGACCCACATGGCCGAGGGCTACGCCCGCGCCACCGGCAGGCCCGGCGTGGTGCTGGTCACCTCCGGC
CCCGGCGCGACCAACACCGTCACCGGCATCACCAACGCCTACATGGACTCCACGCCGCTAGTGGTCTTCACCGGCCAGGT
GCCGAGTTCGCTGATCGGCAACGACGCCTTTCAGGAAGCGGACATCGTCGGCATCACGCGGCCGATCACCAAGCACAACT
TCCTCGTCAAGGATGTGCGCGAGCTGGCTACGACCATCCGCAAGGCATTTTACCTGGCCACCAACGGACGGCCAGGCCCG
GTGCTGGTCGATATGCCCAAAGATGTGCTGAACGCCGAATGCACCTTCGAGTGGCCGGAGAACGTTGACATTCGAGGCTT
CAAGCCGACGATCAAGTGCCACGCCAACCAGGTCAGCAAAGCGGCGAAAATGATCGCCAAAGCCAAGCGTCCGCTCTTCT
ACGTCGGCGGCGGCGTCATCAGCGCGGAGGCATCGGCGGAGCTGCGCAAGCTGGCCATCGACCAGCAGATTCCGGTCACC
ATGACCCTGCAAGGACTCGGCGCGTTCCCCGGCGACCATCCGCTCTCCATGGGAATGCTCGGCATGCACGGCACCTACTG
GGCCAACCAGGCGGTGAGCAACTGTGACCTGTTGATCGCCGTCGGTGCGCGCTTCGACGACCGCGTCACCGGCAAGGTTG
ACACCTTCGCCACCCATGCGTACAAAATCCACAACGACATCGACCCGACCAACGTGGACAAGAACATCAAGGTCGATCTG
CCGGTGGTCGGCGACTCGAAGGACTTCCTCGCCTCGCTCATCGAAGCGATGCCGAAATCGAGGGAGGATCGCAGCGCGTG
GCTCGCCGAAATCGAGAAGTGGCGCAAGCAGTGTCCGCTCGACTACGAGATCGAGCCGGATTCGCTCAAAACCGAGTTCG
TCATCGACGAGGTCTCCAGGCAGACCAAAGGTCACGCGGTGGTTGTTACCGACGTCGGCCAGCACCAGATGTGGACGTCG
CAGTACTACAAGTTCACCGAACCGCGCTCGATCATCACGAGTGGCGGACTCGGCACCATGGGCTTCGGCCTGCCGTCGGC
CATCGGCGCGGCCTTCGGCGTCACCGACCGGCCAGTGTTGCTCTTCAGTGGCGACGGCGGTCTGATGATGAACATCCAGG
AGATGGTCACGGCGGTTTACAACAAACTGCCGATCAAGATTTTCCTGATCAACAACAGCTACCTCGGCATGGTGCGCCAG
TGGCAGGAGCTGTTCCATCAGGAGAAGTACACCTTCACCGACCTGGCGTCGAGCAACCCCGATTTCGTCAAGGTTGCCGA
AGCCTTTGGCTGCAAGGCGATGAGCGCCAGCAATCCGGAAGCAGCGCGCGCCGCCATCACCGAAGCGCTCGCGTACAACG
ACGGGCCAGTGCTGGTTGATTTCAGGGTTATCCGCAAGGATATGGTTTTTCCAATGGTACCCGCCGGCGGCTCGATTTCA
GACATGCTGTTGGCCCGGCTGAATCCGAAAACAATGGTTTAA

Upstream 100 bases:

>100_bases
CGGTGCGGTTCTGAAAAACGATTTTTAACTAATATGAATATGGGGCTGGCCATTGCGCTTCCGGATGACTCGCCTTTCCA
TCGATCAACAGGATTGAACC

Downstream 100 bases:

>100_bases
GCTACTTCTATGAAACATCTTATATCCGTTCTGGTCGAGAACAAGTTCGGCACGCTGAACCGGGTTGCCGCCATGTTCAG
CGCTCGCGGCTTCAATCTCG

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MHNNGEKLIGSEIFFECLRRENVEYIFGYPGGALLKVYETLHDVEDIEHILARHEQGATHMAEGYARATGRPGVVLVTSG
PGATNTVTGITNAYMDSTPLVVFTGQVPSSLIGNDAFQEADIVGITRPITKHNFLVKDVRELATTIRKAFYLATNGRPGP
VLVDMPKDVLNAECTFEWPENVDIRGFKPTIKCHANQVSKAAKMIAKAKRPLFYVGGGVISAEASAELRKLAIDQQIPVT
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVSNCDLLIAVGARFDDRVTGKVDTFATHAYKIHNDIDPTNVDKNIKVDL
PVVGDSKDFLASLIEAMPKSREDRSAWLAEIEKWRKQCPLDYEIEPDSLKTEFVIDEVSRQTKGHAVVVTDVGQHQMWTS
QYYKFTEPRSIITSGGLGTMGFGLPSAIGAAFGVTDRPVLLFSGDGGLMMNIQEMVTAVYNKLPIKIFLINNSYLGMVRQ
WQELFHQEKYTFTDLASSNPDFVKVAEAFGCKAMSASNPEAARAAITEALAYNDGPVLVDFRVIRKDMVFPMVPAGGSIS
DMLLARLNPKTMV

Sequences:

>Translated_573_residues
MHNNGEKLIGSEIFFECLRRENVEYIFGYPGGALLKVYETLHDVEDIEHILARHEQGATHMAEGYARATGRPGVVLVTSG
PGATNTVTGITNAYMDSTPLVVFTGQVPSSLIGNDAFQEADIVGITRPITKHNFLVKDVRELATTIRKAFYLATNGRPGP
VLVDMPKDVLNAECTFEWPENVDIRGFKPTIKCHANQVSKAAKMIAKAKRPLFYVGGGVISAEASAELRKLAIDQQIPVT
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVSNCDLLIAVGARFDDRVTGKVDTFATHAYKIHNDIDPTNVDKNIKVDL
PVVGDSKDFLASLIEAMPKSREDRSAWLAEIEKWRKQCPLDYEIEPDSLKTEFVIDEVSRQTKGHAVVVTDVGQHQMWTS
QYYKFTEPRSIITSGGLGTMGFGLPSAIGAAFGVTDRPVLLFSGDGGLMMNIQEMVTAVYNKLPIKIFLINNSYLGMVRQ
WQELFHQEKYTFTDLASSNPDFVKVAEAFGCKAMSASNPEAARAAITEALAYNDGPVLVDFRVIRKDMVFPMVPAGGSIS
DMLLARLNPKTMV
>Mature_573_residues
MHNNGEKLIGSEIFFECLRRENVEYIFGYPGGALLKVYETLHDVEDIEHILARHEQGATHMAEGYARATGRPGVVLVTSG
PGATNTVTGITNAYMDSTPLVVFTGQVPSSLIGNDAFQEADIVGITRPITKHNFLVKDVRELATTIRKAFYLATNGRPGP
VLVDMPKDVLNAECTFEWPENVDIRGFKPTIKCHANQVSKAAKMIAKAKRPLFYVGGGVISAEASAELRKLAIDQQIPVT
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVSNCDLLIAVGARFDDRVTGKVDTFATHAYKIHNDIDPTNVDKNIKVDL
PVVGDSKDFLASLIEAMPKSREDRSAWLAEIEKWRKQCPLDYEIEPDSLKTEFVIDEVSRQTKGHAVVVTDVGQHQMWTS
QYYKFTEPRSIITSGGLGTMGFGLPSAIGAAFGVTDRPVLLFSGDGGLMMNIQEMVTAVYNKLPIKIFLINNSYLGMVRQ
WQELFHQEKYTFTDLASSNPDFVKVAEAFGCKAMSASNPEAARAAITEALAYNDGPVLVDFRVIRKDMVFPMVPAGGSIS
DMLLARLNPKTMV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=26.4388489208633, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI21361361, Length=600, Percent_Identity=24.5, Blast_Score=140, Evalue=5e-33,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=45.9649122807018, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=44.5632798573975, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=33.641404805915, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=26.2962962962963, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1788716, Length=583, Percent_Identity=25.3859348198971, Blast_Score=146, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=576, Percent_Identity=25.1736111111111, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=25.3097345132743, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=25.3097345132743, Blast_Score=142, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=45.6410256410256, Blast_Score=491, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320816, Length=517, Percent_Identity=24.7582205029014, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=530, Percent_Identity=23.0188679245283, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=537, Percent_Identity=23.6499068901304, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320123, Length=530, Percent_Identity=20.5660377358491, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=522, Percent_Identity=22.4137931034483, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922626, Length=557, Percent_Identity=25.673249551167, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62875; Mature: 62875

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNNGEKLIGSEIFFECLRRENVEYIFGYPGGALLKVYETLHDVEDIEHILARHEQGATH
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGRPGVVLVTSGPGATNTVTGITNAYMDSTPLVVFTGQVPSSLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCHHHHCCCHHCCC
DIVGITRPITKHNFLVKDVRELATTIRKAFYLATNGRPGPVLVDMPKDVLNAECTFEWPE
CEEEEECCCHHCHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCEEEEECCC
NVDIRGFKPTIKCHANQVSKAAKMIAKAKRPLFYVGGGVISAEASAELRKLAIDQQIPVT
CCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEECCEEEECCHHHHHHHHHCCCCCCEE
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVSNCDLLIAVGARFDDRVTGKVDTFATHA
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHEEEE
YKIHNDIDPTNVDKNIKVDLPVVGDSKDFLASLIEAMPKSREDRSAWLAEIEKWRKQCPL
EEECCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DYEIEPDSLKTEFVIDEVSRQTKGHAVVVTDVGQHQMWTSQYYKFTEPRSIITSGGLGTM
CCEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHEEECCCCHHEECCCCCCC
GFGLPSAIGAAFGVTDRPVLLFSGDGGLMMNIQEMVTAVYNKLPIKIFLINNSYLGMVRQ
CCCCHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQELFHQEKYTFTDLASSNPDFVKVAEAFGCKAMSASNPEAARAAITEALAYNDGPVLVD
HHHHHHCCCCEEEHHCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
FRVIRKDMVFPMVPAGGSISDMLLARLNPKTMV
HHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHNNGEKLIGSEIFFECLRRENVEYIFGYPGGALLKVYETLHDVEDIEHILARHEQGATH
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGRPGVVLVTSGPGATNTVTGITNAYMDSTPLVVFTGQVPSSLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCHHHHCCCHHCCC
DIVGITRPITKHNFLVKDVRELATTIRKAFYLATNGRPGPVLVDMPKDVLNAECTFEWPE
CEEEEECCCHHCHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCEEEEECCC
NVDIRGFKPTIKCHANQVSKAAKMIAKAKRPLFYVGGGVISAEASAELRKLAIDQQIPVT
CCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEECCEEEECCHHHHHHHHHCCCCCCEE
MTLQGLGAFPGDHPLSMGMLGMHGTYWANQAVSNCDLLIAVGARFDDRVTGKVDTFATHA
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHEEEE
YKIHNDIDPTNVDKNIKVDLPVVGDSKDFLASLIEAMPKSREDRSAWLAEIEKWRKQCPL
EEECCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DYEIEPDSLKTEFVIDEVSRQTKGHAVVVTDVGQHQMWTSQYYKFTEPRSIITSGGLGTM
CCEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHEEECCCCHHEECCCCCCC
GFGLPSAIGAAFGVTDRPVLLFSGDGGLMMNIQEMVTAVYNKLPIKIFLINNSYLGMVRQ
CCCCHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQELFHQEKYTFTDLASSNPDFVKVAEAFGCKAMSASNPEAARAAITEALAYNDGPVLVD
HHHHHHCCCCEEEHHCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
FRVIRKDMVFPMVPAGGSISDMLLARLNPKTMV
HHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]