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The map label for this gene is ilvD [H]

Identifier: 21673454

GI number: 21673454

Start: 620923

End: 622605

Strand: Reverse

Name: ilvD [H]

Synonym: CT0619

Alternate gene names: 21673454

Gene position: 622605-620923 (Counterclockwise)

Preceding gene: 21673455

Following gene: 21673453

Centisome position: 28.89

GC content: 62.39

Gene sequence:

>1683_bases
ATGAGATCAGATACCATAAAAAAAGGATTTGAAAAGGCTCCGCACCGCAGTCTCCTCAAGGCAACCGGATGCGTTTCGAC
ACGAGATGATTTCTCGAAACCGTTCATCGGCATCTGCAACTCCTTCAACGAGCTGATTCCGGGCCACGCGCATTTGCAGG
AGCTGGGCCGCATCGCCAAGGAAGCCGTGCGCGAGGCTGGCGGCGTGCCGTTCGAGTTCAACACCATCGGCGTCTGCGAC
GGCATTGCGATGGGCCACGTCGGCATGCGCTACTCGCTGGCCAGCCGCGAGCTGATCGCCGACTCGGTCGAGACCGTCGT
CGAAGCGCACCGGCTCGACGGCCTGGTCTGCATCCCAAACTGCGACAAAATCACCCCCGGCATGATGATGGGCGCGCTCA
GGACGAACGTGCCGGTTGTCTTCGTCTCAGGCGGGCCGATGAAGGCCGGGCACACCCCATCGGGCAAAACGGTCGATCTT
ATCTCGGTTTTCGAGGCGGTCGGCAAGTGCAGCACCGGCGAAATCACGGAGGATGAACTCCAGACCGTCGAGGAGTGCGG
CTGCCCAGGATGCGGCTCCTGCTCCGGCATGTTCACGGCCAACTCGATGAACTGCCTCTGCGAAGCGCTCGGTTTCGCGC
TGCCCGGCAACGGCACGATCCTTGCCGCCGATCCGCGACGCAACGAGCTGGTCAAAGCCGCTGCCGGACGCATCATCGAT
CTGGTGAAGAAGGAGGTTCGCCCGCGCCAGATTCTGACCCGTACGTCGATGCTCAACGCCTTCGCGCTCGACCTGGCGAT
GGGCGGCAGCACCAACACGATTCTGCACACGCTGGCCATCGCAAGCGAAGCTGAACTCGACTTCGACTTCTCGGAGCTGA
ACGACCTGTCAGCCAAAACGCCCTATATCTGCAAGGTGAGCCCGGCAACCACCGAGGTACACATCGAGGATGTTGATCGC
GCGGGCGGCATTTCGGCCATTCTGAAGGAGCTGTCGAAGGTTGAGGGGCTGCTCGACCTCTCCGCCCCGACCGTCACCGG
CAAGACGCTCGGCGAGAATATCGCCAGCGCCGAGGTGCTCGACCGCACGGTGATTCGCTCCGTCGAGGAGCCGTATTCGA
CCACCGGCGGTCTGGCAGTGCTCTACGGGAACCTTGCGCCGAACGGCGCAGTTGTCAAAACCGGCGCGGTCAGCCCGGCG
ATGATGAAGCACACCGGCCCGGCGAAGGTCTATGACTGCCAGGACGACGCCATTGCGGGCATTATGAATGGCGACGTCAA
GTCGGGCGATGTGGTGGTGATCCGCTACGAAGGCCCGCGCGGCGGCCCCGGGATGCCGGAGATGCTCTCGCCAACCAGCG
CCATCATAGGCCGCGGCCTCGGCGATTCAGTCGCGCTCATCACCGACGGGCGCTTCTCCGGCGGATCGCGTGGCGCCTGT
GTAGGTCACGTCTCGCCCGAAGCCGCCGACCGCGGCCCGATCGCGGCGGTACAAACCGGCGACATGATCACCATCGACAT
ACCGGCTCGTTCGATGACGGTAGCACTCGATGACGAGACCATCCGCCAGCGCATCGAGGCGTTGCCAAAGTTCGAGCCGA
AAATCAAAAAAGGGTATCTGGCACGATACGCACGAATGGTCACCTCGGCCAATACCGGTGCGGTTCTGAAAAACGATTTT
TAA

Upstream 100 bases:

>100_bases
TATTTTCCGCGACAATACATCAGACTTCAAGGCTTCGGGACAGGCAGGCCCGGACTTTTGTTTTTCTTTGTTTTTCAATT
ACTAACCATCGACTTGACCC

Downstream 100 bases:

>100_bases
CTAATATGAATATGGGGCTGGCCATTGCGCTTCCGGATGACTCGCCTTTCCATCGATCAACAGGATTGAACCATGCATAA
CAACGGAGAGAAACTGATCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MRSDTIKKGFEKAPHRSLLKATGCVSTRDDFSKPFIGICNSFNELIPGHAHLQELGRIAKEAVREAGGVPFEFNTIGVCD
GIAMGHVGMRYSLASRELIADSVETVVEAHRLDGLVCIPNCDKITPGMMMGALRTNVPVVFVSGGPMKAGHTPSGKTVDL
ISVFEAVGKCSTGEITEDELQTVEECGCPGCGSCSGMFTANSMNCLCEALGFALPGNGTILAADPRRNELVKAAAGRIID
LVKKEVRPRQILTRTSMLNAFALDLAMGGSTNTILHTLAIASEAELDFDFSELNDLSAKTPYICKVSPATTEVHIEDVDR
AGGISAILKELSKVEGLLDLSAPTVTGKTLGENIASAEVLDRTVIRSVEEPYSTTGGLAVLYGNLAPNGAVVKTGAVSPA
MMKHTGPAKVYDCQDDAIAGIMNGDVKSGDVVVIRYEGPRGGPGMPEMLSPTSAIIGRGLGDSVALITDGRFSGGSRGAC
VGHVSPEAADRGPIAAVQTGDMITIDIPARSMTVALDDETIRQRIEALPKFEPKIKKGYLARYARMVTSANTGAVLKNDF

Sequences:

>Translated_560_residues
MRSDTIKKGFEKAPHRSLLKATGCVSTRDDFSKPFIGICNSFNELIPGHAHLQELGRIAKEAVREAGGVPFEFNTIGVCD
GIAMGHVGMRYSLASRELIADSVETVVEAHRLDGLVCIPNCDKITPGMMMGALRTNVPVVFVSGGPMKAGHTPSGKTVDL
ISVFEAVGKCSTGEITEDELQTVEECGCPGCGSCSGMFTANSMNCLCEALGFALPGNGTILAADPRRNELVKAAAGRIID
LVKKEVRPRQILTRTSMLNAFALDLAMGGSTNTILHTLAIASEAELDFDFSELNDLSAKTPYICKVSPATTEVHIEDVDR
AGGISAILKELSKVEGLLDLSAPTVTGKTLGENIASAEVLDRTVIRSVEEPYSTTGGLAVLYGNLAPNGAVVKTGAVSPA
MMKHTGPAKVYDCQDDAIAGIMNGDVKSGDVVVIRYEGPRGGPGMPEMLSPTSAIIGRGLGDSVALITDGRFSGGSRGAC
VGHVSPEAADRGPIAAVQTGDMITIDIPARSMTVALDDETIRQRIEALPKFEPKIKKGYLARYARMVTSANTGAVLKNDF
>Mature_560_residues
MRSDTIKKGFEKAPHRSLLKATGCVSTRDDFSKPFIGICNSFNELIPGHAHLQELGRIAKEAVREAGGVPFEFNTIGVCD
GIAMGHVGMRYSLASRELIADSVETVVEAHRLDGLVCIPNCDKITPGMMMGALRTNVPVVFVSGGPMKAGHTPSGKTVDL
ISVFEAVGKCSTGEITEDELQTVEECGCPGCGSCSGMFTANSMNCLCEALGFALPGNGTILAADPRRNELVKAAAGRIID
LVKKEVRPRQILTRTSMLNAFALDLAMGGSTNTILHTLAIASEAELDFDFSELNDLSAKTPYICKVSPATTEVHIEDVDR
AGGISAILKELSKVEGLLDLSAPTVTGKTLGENIASAEVLDRTVIRSVEEPYSTTGGLAVLYGNLAPNGAVVKTGAVSPA
MMKHTGPAKVYDCQDDAIAGIMNGDVKSGDVVVIRYEGPRGGPGMPEMLSPTSAIIGRGLGDSVALITDGRFSGGSRGAC
VGHVSPEAADRGPIAAVQTGDMITIDIPARSMTVALDDETIRQRIEALPKFEPKIKKGYLARYARMVTSANTGAVLKNDF

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=607, Percent_Identity=48.2701812191104, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1788157, Length=525, Percent_Identity=32, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI2367371, Length=507, Percent_Identity=32.3471400394477, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1786464, Length=503, Percent_Identity=31.2127236580517, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322476, Length=554, Percent_Identity=39.5306859205776, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 58916; Mature: 58916

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDTIKKGFEKAPHRSLLKATGCVSTRDDFSKPFIGICNSFNELIPGHAHLQELGRIAK
CCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
EAVREAGGVPFEFNTIGVCDGIAMGHVGMRYSLASRELIADSVETVVEAHRLDGLVCIPN
HHHHHHCCCCEEECEEECCCCHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEECCC
CDKITPGMMMGALRTNVPVVFVSGGPMKAGHTPSGKTVDLISVFEAVGKCSTGEITEDEL
CCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCHHHH
QTVEECGCPGCGSCSGMFTANSMNCLCEALGFALPGNGTILAADPRRNELVKAAAGRIID
HHHHHCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
LVKKEVRPRQILTRTSMLNAFALDLAMGGSTNTILHTLAIASEAELDFDFSELNDLSAKT
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCC
PYICKVSPATTEVHIEDVDRAGGISAILKELSKVEGLLDLSAPTVTGKTLGENIASAEVL
CEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHH
DRTVIRSVEEPYSTTGGLAVLYGNLAPNGAVVKTGAVSPAMMKHTGPAKVYDCQDDAIAG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHCCCCCEEEECCCCCEEE
IMNGDVKSGDVVVIRYEGPRGGPGMPEMLSPTSAIIGRGLGDSVALITDGRFSGGSRGAC
EECCCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCEE
VGHVSPEAADRGPIAAVQTGDMITIDIPARSMTVALDDETIRQRIEALPKFEPKIKKGYL
EECCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHH
ARYARMVTSANTGAVLKNDF
HHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure
MRSDTIKKGFEKAPHRSLLKATGCVSTRDDFSKPFIGICNSFNELIPGHAHLQELGRIAK
CCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
EAVREAGGVPFEFNTIGVCDGIAMGHVGMRYSLASRELIADSVETVVEAHRLDGLVCIPN
HHHHHHCCCCEEECEEECCCCHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEECCC
CDKITPGMMMGALRTNVPVVFVSGGPMKAGHTPSGKTVDLISVFEAVGKCSTGEITEDEL
CCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCHHHH
QTVEECGCPGCGSCSGMFTANSMNCLCEALGFALPGNGTILAADPRRNELVKAAAGRIID
HHHHHCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
LVKKEVRPRQILTRTSMLNAFALDLAMGGSTNTILHTLAIASEAELDFDFSELNDLSAKT
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCC
PYICKVSPATTEVHIEDVDRAGGISAILKELSKVEGLLDLSAPTVTGKTLGENIASAEVL
CEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHH
DRTVIRSVEEPYSTTGGLAVLYGNLAPNGAVVKTGAVSPAMMKHTGPAKVYDCQDDAIAG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHCCCCCEEEECCCCCEEE
IMNGDVKSGDVVVIRYEGPRGGPGMPEMLSPTSAIIGRGLGDSVALITDGRFSGGSRGAC
EECCCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCEE
VGHVSPEAADRGPIAAVQTGDMITIDIPARSMTVALDDETIRQRIEALPKFEPKIKKGYL
EECCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHH
ARYARMVTSANTGAVLKNDF
HHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA