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The map label for this gene is leuB

Identifier: 21673450

GI number: 21673450

Start: 616537

End: 617595

Strand: Reverse

Name: leuB

Synonym: CT0615

Alternate gene names: 21673450

Gene position: 617595-616537 (Counterclockwise)

Preceding gene: 21673451

Following gene: 21673449

Centisome position: 28.66

GC content: 59.96

Gene sequence:

>1059_bases
ATGATGTATAAAATTGTCTCTATCCCCGGCGACGGCATCGGCCCCGAAGTGGTAGCCGGTGCGCTCGACGTGCTGAACGC
CGTCGCGAAAAAGCACGGCTTTGAAGTTTCGGTCGAGGAGCACCTGTTCGGCGGCGCGTCGTATGACGTGCACGGCTCCA
TGCTGACCGACGAGACGCTCGAGGCATGCAAGAACTGCGACGCGGTGCTGCTTGGCGCCGTCGGCGGCTACAAATGGGAG
AACCTGCCGCACGACAAAAAGCCTGAAGCGGCCCTGCTGAAAATCCGCAAGGAGCTCGGCCTGTTTGCAAACTTGCGTCC
GGCGAGGGTGTACGATGCTCTCGTGGCATCATCGACGCTGAAAACTGAAGTGGTGCAGGGCACTGACTTTATGGTGTTCC
GTGAACTGACCGGCGGCATCTACTTCGGTCAGCCGAGAGGCTACGACGAAACGCGCGGCTGGAACACAATGGTTTACGAG
CGTTACGAGGTAGAGCGCATCGCGCGGCTTGCGTTCGAATACGCGCAGAAACGCGGCAACGCCAAGGTCACCTCGATCGA
CAAGGCCAACGTACTTGAAGTCTCACAGTTCTGGCGCAACATCGTGCATGAAGTGCACCAGGATTTCCCGGAAATCGAGC
TGGTCGATATGTACGTTGACAACGCCGCAATGCAGGTGGTGCGCAACCCGAAGCAGTTTGAGGTGATCGTGACGAGCAAC
CTCTTCGGCGACATCCTGAGCGACATCTCCGGCATGATCACCGGCAGCCTCGGCATGTTGCCCTCGGCCAGCATCGGCTC
GGAGCACGCGCTCTACGAACCGATTCACGGCAGCGCTCCCGACATCGCGGGCCAGAACAAGGCCAACCCGATCGCGACCA
TTGCATCGGTGGCCATGATGTTCGAGAACAGCTTCAACCGGCCCGAAGTGGCGGCTGACATTTACGCGGCCATCGAAGGC
GCGCTTGCTGCAGGCTTCCGCACGGGCGACATCGCCGCAGCGGGCGAGGCGATCTCTTCGACAACAGAAATGACGGCGGC
TATCGTCGCCCGAATTTGA

Upstream 100 bases:

>100_bases
TGAACGAGTTGCGTGAGAGCAACCGCAACCACCCCATCGAAGTCGTCGGCGCGAAGCTGCGCGGCATGATGAGCTGGCTG
AAAAAGAAATAAGCGGGAGG

Downstream 100 bases:

>100_bases
CGGCGAACAACGCAAAGAAAACGACAGAGCACATGCGGCGGTGAGCCGCAACGAGAAAAGCCGGAGCTCTGCAGGTCTCC
GGCTTTTCAGTTTTTGACGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MMYKIVSIPGDGIGPEVVAGALDVLNAVAKKHGFEVSVEEHLFGGASYDVHGSMLTDETLEACKNCDAVLLGAVGGYKWE
NLPHDKKPEAALLKIRKELGLFANLRPARVYDALVASSTLKTEVVQGTDFMVFRELTGGIYFGQPRGYDETRGWNTMVYE
RYEVERIARLAFEYAQKRGNAKVTSIDKANVLEVSQFWRNIVHEVHQDFPEIELVDMYVDNAAMQVVRNPKQFEVIVTSN
LFGDILSDISGMITGSLGMLPSASIGSEHALYEPIHGSAPDIAGQNKANPIATIASVAMMFENSFNRPEVAADIYAAIEG
ALAAGFRTGDIAAAGEAISSTTEMTAAIVARI

Sequences:

>Translated_352_residues
MMYKIVSIPGDGIGPEVVAGALDVLNAVAKKHGFEVSVEEHLFGGASYDVHGSMLTDETLEACKNCDAVLLGAVGGYKWE
NLPHDKKPEAALLKIRKELGLFANLRPARVYDALVASSTLKTEVVQGTDFMVFRELTGGIYFGQPRGYDETRGWNTMVYE
RYEVERIARLAFEYAQKRGNAKVTSIDKANVLEVSQFWRNIVHEVHQDFPEIELVDMYVDNAAMQVVRNPKQFEVIVTSN
LFGDILSDISGMITGSLGMLPSASIGSEHALYEPIHGSAPDIAGQNKANPIATIASVAMMFENSFNRPEVAADIYAAIEG
ALAAGFRTGDIAAAGEAISSTTEMTAAIVARI
>Mature_352_residues
MMYKIVSIPGDGIGPEVVAGALDVLNAVAKKHGFEVSVEEHLFGGASYDVHGSMLTDETLEACKNCDAVLLGAVGGYKWE
NLPHDKKPEAALLKIRKELGLFANLRPARVYDALVASSTLKTEVVQGTDFMVFRELTGGIYFGQPRGYDETRGWNTMVYE
RYEVERIARLAFEYAQKRGNAKVTSIDKANVLEVSQFWRNIVHEVHQDFPEIELVDMYVDNAAMQVVRNPKQFEVIVTSN
LFGDILSDISGMITGSLGMLPSASIGSEHALYEPIHGSAPDIAGQNKANPIATIASVAMMFENSFNRPEVAADIYAAIEG
ALAAGFRTGDIAAAGEAISSTTEMTAAIVARI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=302, Percent_Identity=32.1192052980132, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI28178821, Length=312, Percent_Identity=28.8461538461538, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI28178816, Length=312, Percent_Identity=28.8461538461538, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI4758582, Length=304, Percent_Identity=28.2894736842105, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI28178838, Length=304, Percent_Identity=28.2894736842105, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI28178819, Length=149, Percent_Identity=35.5704697986577, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=51.9662921348315, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1788101, Length=363, Percent_Identity=37.465564738292, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1787381, Length=375, Percent_Identity=28, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=32.7586206896552, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17550882, Length=306, Percent_Identity=29.7385620915033, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=302, Percent_Identity=31.1258278145695, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=311, Percent_Identity=30.8681672025724, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=46.6850828729282, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=363, Percent_Identity=34.9862258953168, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=357, Percent_Identity=31.9327731092437, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=358, Percent_Identity=31.0055865921788, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643268, Length=347, Percent_Identity=31.1239193083574, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643270, Length=347, Percent_Identity=31.1239193083574, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=28.6135693215339, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=28.6135693215339, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=28.6135693215339, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=28.6135693215339, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI161078639, Length=346, Percent_Identity=28.9017341040462, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24661184, Length=308, Percent_Identity=29.5454545454545, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=30.0578034682081, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=30.0578034682081, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20130355, Length=302, Percent_Identity=23.1788079470199, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_CHLTE (P59028)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661515.1
- ProteinModelPortal:   P59028
- SMR:   P59028
- GeneID:   1006259
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0615
- NMPDR:   fig|194439.1.peg.609
- TIGR:   CT0615
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   CTEP194439:CT_0615-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38170; Mature: 38170

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 134-134 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYKIVSIPGDGIGPEVVAGALDVLNAVAKKHGFEVSVEEHLFGGASYDVHGSMLTDETL
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCCCCCCCHHHH
EACKNCDAVLLGAVGGYKWENLPHDKKPEAALLKIRKELGLFANLRPARVYDALVASSTL
HHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTEVVQGTDFMVFRELTGGIYFGQPRGYDETRGWNTMVYERYEVERIARLAFEYAQKRGN
HHHHHCCCCCEEEHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AKVTSIDKANVLEVSQFWRNIVHEVHQDFPEIELVDMYVDNAAMQVVRNPKQFEVIVTSN
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEECH
LFGDILSDISGMITGSLGMLPSASIGSEHALYEPIHGSAPDIAGQNKANPIATIASVAMM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
FENSFNRPEVAADIYAAIEGALAAGFRTGDIAAAGEAISSTTEMTAAIVARI
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMYKIVSIPGDGIGPEVVAGALDVLNAVAKKHGFEVSVEEHLFGGASYDVHGSMLTDETL
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCCCCCCCHHHH
EACKNCDAVLLGAVGGYKWENLPHDKKPEAALLKIRKELGLFANLRPARVYDALVASSTL
HHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTEVVQGTDFMVFRELTGGIYFGQPRGYDETRGWNTMVYERYEVERIARLAFEYAQKRGN
HHHHHCCCCCEEEHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AKVTSIDKANVLEVSQFWRNIVHEVHQDFPEIELVDMYVDNAAMQVVRNPKQFEVIVTSN
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEECH
LFGDILSDISGMITGSLGMLPSASIGSEHALYEPIHGSAPDIAGQNKANPIATIASVAMM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
FENSFNRPEVAADIYAAIEGALAAGFRTGDIAAAGEAISSTTEMTAAIVARI
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901