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The map label for this gene is oprM [H]

Identifier: 21673389

GI number: 21673389

Start: 561726

End: 563228

Strand: Direct

Name: oprM [H]

Synonym: CT0554

Alternate gene names: 21673389

Gene position: 561726-563228 (Clockwise)

Preceding gene: 21673388

Following gene: 21673392

Centisome position: 26.07

GC content: 62.74

Gene sequence:

>1503_bases
TTGGTGAACAGGCCGCGAAAAACCAGTAACCGGATGAGAACCGTGTCGAAAAAGATCGCTTCACGCGGCGTCGTACTGCT
TGCCGTTCCCGCGCTTCTGCTGTCGGGATGCGCGGCAGGACCGGACTTCGTCAAGCCGGAGGCTCCGGCAGTCAAAAGCT
ACGACCAGCAACCACTTCCGAAGCCGGTCGATGGCGGGCAGCGCTTTGACGAGGGCGTTGGACCTGCTGCATACTGGTGG
AAATCGTTCGGTTCCACCCAGATCGACTCCATTGTCGAAGAGGGGCTGGTGGGTAATCCGGGCTTGCAGGCTGCCGAGGC
AAGCCTCAAAGCGAGCCGGGAGAATCTCCGCGCCGGGGCTGGGATTTTTTATCCGCAGGCAAGTGCGACCTTTTCGCAGA
CGCGGGAAACTTCTTCGCCAGCCACGACAGCGGGCTCGGGCGCCATCGTTAACCTCTCGACCCTCTCCGCGTCAGTGAGC
TATGCGCTCGACCTCTTCGGCGGGCAGCGGCGCAGCGTCGAGGCGCTCGGAGCGCAGGTGGACGTGCAGCGGGCGCAGAC
TCTCGGTGTGTACATGACGCTGACTGGCAACATCGTTAACACGAGTATTGCCATTGCCGCCTATCGCGAGGAACTCGACG
AGTACGAGCAGCTCATCGCCCTCGAAAAGGATCAGCTTTCGATAGCCACGAAGCAGTACCAGGCGGGTGTAGCACCCTAC
AGCTCCGTGCTGGCGTTACGGAGCCAGATTGCCTCGCTGGAGGCCTCCGTGCCGCCAGTCAGGCAGAAGTTAAGTCAGGC
CGAGCACCTGCTTGCGACGCTTGCAGGAAAGACTCCGGGGGAGTGGGTGAGGCCCGATCTGCGCCTCTCAGGCATTTCCC
TGCCAGAGCGTGTGCCGCTCTCGCTGCCATCGGAGCTGGTGCGCCAGCGCCCGGACATCCTCGCCGCCGAAGCGGAGCTG
CACGCGGCCAACGCCGAAATCGGCGTTGCCACGGCAGCGCTCTTCCCGAGCTTCACGCTCACTGGCAGTTACGGGCGCAC
GGCCAGCCAGCCGCATGAGCTTTCCGATCCACTCAACCGATTCTGGTCAATTGGTGGCAACATCGCCGCGCCGATCTTCA
ACGGCGGCTCGCTCCGGGCCAAACGCCGGGCTGCGATCGCCACGCGAAACCAGGCGCTCGCGTTGTACCGCCAGACCGTG
CTTGCCGCCTTCGCCCAGGTTGCCGACCTCATCCGCGCGCTTGAGCACGACGCACAGCAAGCCGAAGCCGAAAAGCAGGC
GGTTGAGAGTGCAAAACTTTCGCTCGACCTCGTTCAGGCCAACTACAAGGCGGGCATGGTCAACTACGAGCAGGTGATTC
TTGCTGACATTCAGTACCGGCAAGCGAAAATCGGTTACCTGCAAGCCCGCGCTCAGCAACTGCAAGACACCGCCGCCATG
TACGTCGCTCTTGGTGGGGGCTGGCCGCATGATGGGGTAGCGAGGGAGAAAGCAGGCTTGTAG

Upstream 100 bases:

>100_bases
CGCACCGAGCGGGTGGATGTCCGGGTGCTGCCGGTCATCTTCAGCTTCAAGAAGCCAGCAAACCTGACCCTCTATCCCGG
TCAGCTGGTCGATATTTACA

Downstream 100 bases:

>100_bases
GGCTTGAGTGAGAGCCATAAAACAAAAAAGACCTGCAAGTCGAAACTCGCAAGTCATTCTGCTTTATGGTACACCCGAGA
GGATTCGAACCCCTAACCTT

Product: ABC-type export system, outer membrane channel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MVNRPRKTSNRMRTVSKKIASRGVVLLAVPALLLSGCAAGPDFVKPEAPAVKSYDQQPLPKPVDGGQRFDEGVGPAAYWW
KSFGSTQIDSIVEEGLVGNPGLQAAEASLKASRENLRAGAGIFYPQASATFSQTRETSSPATTAGSGAIVNLSTLSASVS
YALDLFGGQRRSVEALGAQVDVQRAQTLGVYMTLTGNIVNTSIAIAAYREELDEYEQLIALEKDQLSIATKQYQAGVAPY
SSVLALRSQIASLEASVPPVRQKLSQAEHLLATLAGKTPGEWVRPDLRLSGISLPERVPLSLPSELVRQRPDILAAEAEL
HAANAEIGVATAALFPSFTLTGSYGRTASQPHELSDPLNRFWSIGGNIAAPIFNGGSLRAKRRAAIATRNQALALYRQTV
LAAFAQVADLIRALEHDAQQAEAEKQAVESAKLSLDLVQANYKAGMVNYEQVILADIQYRQAKIGYLQARAQQLQDTAAM
YVALGGGWPHDGVAREKAGL

Sequences:

>Translated_500_residues
MVNRPRKTSNRMRTVSKKIASRGVVLLAVPALLLSGCAAGPDFVKPEAPAVKSYDQQPLPKPVDGGQRFDEGVGPAAYWW
KSFGSTQIDSIVEEGLVGNPGLQAAEASLKASRENLRAGAGIFYPQASATFSQTRETSSPATTAGSGAIVNLSTLSASVS
YALDLFGGQRRSVEALGAQVDVQRAQTLGVYMTLTGNIVNTSIAIAAYREELDEYEQLIALEKDQLSIATKQYQAGVAPY
SSVLALRSQIASLEASVPPVRQKLSQAEHLLATLAGKTPGEWVRPDLRLSGISLPERVPLSLPSELVRQRPDILAAEAEL
HAANAEIGVATAALFPSFTLTGSYGRTASQPHELSDPLNRFWSIGGNIAAPIFNGGSLRAKRRAAIATRNQALALYRQTV
LAAFAQVADLIRALEHDAQQAEAEKQAVESAKLSLDLVQANYKAGMVNYEQVILADIQYRQAKIGYLQARAQQLQDTAAM
YVALGGGWPHDGVAREKAGL
>Mature_500_residues
MVNRPRKTSNRMRTVSKKIASRGVVLLAVPALLLSGCAAGPDFVKPEAPAVKSYDQQPLPKPVDGGQRFDEGVGPAAYWW
KSFGSTQIDSIVEEGLVGNPGLQAAEASLKASRENLRAGAGIFYPQASATFSQTRETSSPATTAGSGAIVNLSTLSASVS
YALDLFGGQRRSVEALGAQVDVQRAQTLGVYMTLTGNIVNTSIAIAAYREELDEYEQLIALEKDQLSIATKQYQAGVAPY
SSVLALRSQIASLEASVPPVRQKLSQAEHLLATLAGKTPGEWVRPDLRLSGISLPERVPLSLPSELVRQRPDILAAEAEL
HAANAEIGVATAALFPSFTLTGSYGRTASQPHELSDPLNRFWSIGGNIAAPIFNGGSLRAKRRAAIATRNQALALYRQTV
LAAFAQVADLIRALEHDAQQAEAEKQAVESAKLSLDLVQANYKAGMVNYEQVILADIQYRQAKIGYLQARAQQLQDTAAM
YVALGGGWPHDGVAREKAGL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=476, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI2367348, Length=491, Percent_Identity=24.0325865580448, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53359; Mature: 53359

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNRPRKTSNRMRTVSKKIASRGVVLLAVPALLLSGCAAGPDFVKPEAPAVKSYDQQPLP
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCC
KPVDGGQRFDEGVGPAAYWWKSFGSTQIDSIVEEGLVGNPGLQAAEASLKASRENLRAGA
CCCCCCHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
GIFYPQASATFSQTRETSSPATTAGSGAIVNLSTLSASVSYALDLFGGQRRSVEALGAQV
CEECCCCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCHH
DVQRAQTLGVYMTLTGNIVNTSIAIAAYREELDEYEQLIALEKDQLSIATKQYQAGVAPY
HHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHH
SSVLALRSQIASLEASVPPVRQKLSQAEHLLATLAGKTPGEWVRPDLRLSGISLPERVPL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCC
SLPSELVRQRPDILAAEAELHAANAEIGVATAALFPSFTLTGSYGRTASQPHELSDPLNR
CCCHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
FWSIGGNIAAPIFNGGSLRAKRRAAIATRNQALALYRQTVLAAFAQVADLIRALEHDAQQ
HHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAEKQAVESAKLSLDLVQANYKAGMVNYEQVILADIQYRQAKIGYLQARAQQLQDTAAM
HHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
YVALGGGWPHDGVAREKAGL
EEEECCCCCCCCCCHHHCCC
>Mature Secondary Structure
MVNRPRKTSNRMRTVSKKIASRGVVLLAVPALLLSGCAAGPDFVKPEAPAVKSYDQQPLP
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCC
KPVDGGQRFDEGVGPAAYWWKSFGSTQIDSIVEEGLVGNPGLQAAEASLKASRENLRAGA
CCCCCCHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
GIFYPQASATFSQTRETSSPATTAGSGAIVNLSTLSASVSYALDLFGGQRRSVEALGAQV
CEECCCCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCHH
DVQRAQTLGVYMTLTGNIVNTSIAIAAYREELDEYEQLIALEKDQLSIATKQYQAGVAPY
HHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHH
SSVLALRSQIASLEASVPPVRQKLSQAEHLLATLAGKTPGEWVRPDLRLSGISLPERVPL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCC
SLPSELVRQRPDILAAEAELHAANAEIGVATAALFPSFTLTGSYGRTASQPHELSDPLNR
CCCHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
FWSIGGNIAAPIFNGGSLRAKRRAAIATRNQALALYRQTVLAAFAQVADLIRALEHDAQQ
HHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEAEKQAVESAKLSLDLVQANYKAGMVNYEQVILADIQYRQAKIGYLQARAQQLQDTAAM
HHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
YVALGGGWPHDGVAREKAGL
EEEECCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]