Click here to switch to the map view.

The map label for this gene is queA-1

Identifier: 21673380

GI number: 21673380

Start: 553309

End: 554331

Strand: Reverse

Name: queA-1

Synonym: CT0545

Alternate gene names: 21673380

Gene position: 554331-553309 (Counterclockwise)

Preceding gene: 21673381

Following gene: 21673378

Centisome position: 25.72

GC content: 61.0

Gene sequence:

>1023_bases
ATGAAGGTCAGCGATTTCGATTACGCCCTTCCCGAAGAGCGCATCGCCTGTTATCCTCCCGCAAAACGCGGTTCGACGAG
ACTGATTGTGCTCAATCGGGCAACCGGATCGATCACCCATTCAGCCTACGCCTCACTGCACGAAGAGCTACGGCCCGGCG
ATCTGCTGGTACTGAACAACAGCCGCGTGATCCGCGCTCGGCTCATCGCCCACAAGCCAACCGGCGCCCGCATCGAACTG
ATGCTGCTCGAAAAGCATGAGGAAGCGCAGAACCTCGCGCTCTACCGGGGACGCCTCCGTATCGGCGACCGGCTGCTGGC
GCACGGCGTCGAACTCTCAGTCGAAGCGCTGCCCAGCGACGGCATCGCGCGCCTCTCCTGCGCCACCGGAAACCTCGCCG
ATCTTTTCGACGCGCACGGCTCCGTACCGATTCCGCCCTATCTCAAGCGCGATGCCGAGGAGCTTGACCGCGAGCGCTAC
CAGACGGTCTTTGCCGAGCTGCCCGGTTCGGTAGCCGCGCCGACAGCATCTCTTAATCTCACCGATGAATTGCTCGATAA
AATCAATGCAAAAGGTGTCGGAATTGCCCACGTGACGCTGCACGTCGGCCTAGGCACCTTCCTACCGATCCGTTCGGAGC
GCTTCGAAGAGCACGTCATGCACCGGGAATTCTACAACATCCCGCAAACGGCCGCTCGAAAGATTGGCGAGACCAAAGCC
TCTGGCGGACGTGTGGTCGCCGCCGGCACCACCGTAACCCGCGCGCTCGAACACGCCGCGCCGAAGCTCATCGAGAGCGG
CTTCACACAGGAGGTGAGCAGCGAGGCGGACATCTTCATCTATCCCGGCTACGAGTTCAGAATCATCGACGCGCTCATCA
CCAACTTCCACGCCCCCCGCTCTACGGTACTCATGCTCACCGCCGCCTTCGCCGAAAAGGAGCTGCTCATGCGAGCCTAC
CACGAAGCCCTCGACAACGGCTACCGTTTCCTCAGCTATGGCGACAGTATGTTTATCAGTTGA

Upstream 100 bases:

>100_bases
AGCTTGCCCGCACCGGGGAGAAGATCGCCCGCTTCGCCGACCATGAAGGGCTTCAGGCTCACGCCGAGGCGGTTCGCGTC
AGACTGAAACACCTGTAACA

Downstream 100 bases:

>100_bases
TAATTATTGCCCACAGGGACAGGCTCCTGAGGCTACCCTGAATCCTTATAAAATCATCGCCTTGCCATACATTCCGTTTT
CAATCAGCGCAATATTTTTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKVSDFDYALPEERIACYPPAKRGSTRLIVLNRATGSITHSAYASLHEELRPGDLLVLNNSRVIRARLIAHKPTGARIEL
MLLEKHEEAQNLALYRGRLRIGDRLLAHGVELSVEALPSDGIARLSCATGNLADLFDAHGSVPIPPYLKRDAEELDRERY
QTVFAELPGSVAAPTASLNLTDELLDKINAKGVGIAHVTLHVGLGTFLPIRSERFEEHVMHREFYNIPQTAARKIGETKA
SGGRVVAAGTTVTRALEHAAPKLIESGFTQEVSSEADIFIYPGYEFRIIDALITNFHAPRSTVLMLTAAFAEKELLMRAY
HEALDNGYRFLSYGDSMFIS

Sequences:

>Translated_340_residues
MKVSDFDYALPEERIACYPPAKRGSTRLIVLNRATGSITHSAYASLHEELRPGDLLVLNNSRVIRARLIAHKPTGARIEL
MLLEKHEEAQNLALYRGRLRIGDRLLAHGVELSVEALPSDGIARLSCATGNLADLFDAHGSVPIPPYLKRDAEELDRERY
QTVFAELPGSVAAPTASLNLTDELLDKINAKGVGIAHVTLHVGLGTFLPIRSERFEEHVMHREFYNIPQTAARKIGETKA
SGGRVVAAGTTVTRALEHAAPKLIESGFTQEVSSEADIFIYPGYEFRIIDALITNFHAPRSTVLMLTAAFAEKELLMRAY
HEALDNGYRFLSYGDSMFIS
>Mature_340_residues
MKVSDFDYALPEERIACYPPAKRGSTRLIVLNRATGSITHSAYASLHEELRPGDLLVLNNSRVIRARLIAHKPTGARIEL
MLLEKHEEAQNLALYRGRLRIGDRLLAHGVELSVEALPSDGIARLSCATGNLADLFDAHGSVPIPPYLKRDAEELDRERY
QTVFAELPGSVAAPTASLNLTDELLDKINAKGVGIAHVTLHVGLGTFLPIRSERFEEHVMHREFYNIPQTAARKIGETKA
SGGRVVAAGTTVTRALEHAAPKLIESGFTQEVSSEADIFIYPGYEFRIIDALITNFHAPRSTVLMLTAAFAEKELLMRAY
HEALDNGYRFLSYGDSMFIS

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=43.6416184971098, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_CHLTE (Q8KEY7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661445.1
- GeneID:   1007598
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0545
- NMPDR:   fig|194439.1.peg.539
- TIGR:   CT0545
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   CLSK637367
- BioCyc:   CTEP194439:CT_0545-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37547; Mature: 37547

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSDFDYALPEERIACYPPAKRGSTRLIVLNRATGSITHSAYASLHEELRPGDLLVLNN
CCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
SRVIRARLIAHKPTGARIELMLLEKHEEAQNLALYRGRLRIGDRLLAHGVELSVEALPSD
CCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCC
GIARLSCATGNLADLFDAHGSVPIPPYLKRDAEELDRERYQTVFAELPGSVAAPTASLNL
CCEEEEECCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TDELLDKINAKGVGIAHVTLHVGLGTFLPIRSERFEEHVMHREFYNIPQTAARKIGETKA
HHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
SGGRVVAAGTTVTRALEHAAPKLIESGFTQEVSSEADIFIYPGYEFRIIDALITNFHAPR
CCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCEEEHHHHHHCCCCCC
STVLMLTAAFAEKELLMRAYHEALDNGYRFLSYGDSMFIS
HHEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECC
>Mature Secondary Structure
MKVSDFDYALPEERIACYPPAKRGSTRLIVLNRATGSITHSAYASLHEELRPGDLLVLNN
CCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECC
SRVIRARLIAHKPTGARIELMLLEKHEEAQNLALYRGRLRIGDRLLAHGVELSVEALPSD
CCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCC
GIARLSCATGNLADLFDAHGSVPIPPYLKRDAEELDRERYQTVFAELPGSVAAPTASLNL
CCEEEEECCCCHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
TDELLDKINAKGVGIAHVTLHVGLGTFLPIRSERFEEHVMHREFYNIPQTAARKIGETKA
HHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
SGGRVVAAGTTVTRALEHAAPKLIESGFTQEVSSEADIFIYPGYEFRIIDALITNFHAPR
CCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCEEEHHHHHHCCCCCC
STVLMLTAAFAEKELLMRAYHEALDNGYRFLSYGDSMFIS
HHEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901