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The map label for this gene is psrB [H]

Identifier: 21673330

GI number: 21673330

Start: 507170

End: 507769

Strand: Reverse

Name: psrB [H]

Synonym: CT0495

Alternate gene names: 21673330

Gene position: 507769-507170 (Counterclockwise)

Preceding gene: 21673331

Following gene: 21673329

Centisome position: 23.56

GC content: 62.17

Gene sequence:

>600_bases
ATGGCCCGTTATGGAATGGTAATGGATATGCGTACCTGCGTCGGCTGTCAGGCCTGCATGGCGGCGTGTTCGACCGAAAA
CCAGACCCCGTTCTGGAGCGAGAAGTTTCGGACGCATGTGGAGGACAAGGAGACAGGAGCCTTCCCCGATGTACGCCGGG
TGCAGCTTCCGCGCCTCTGCATGCACTGCGAAAACACCCCCTGCCTGTCGGCCTGCCCGACCGGGGCGACCCATATGAAC
AAGGACGGCATCGTGCTGGTCAACTATGACCGTTGCATCGGCTGCTACGCCTGCTGCATCGCCTGCCCGTACGACGCCCG
CTACGCCTACGACAGCGAGGATGTCCAGAAGGAGCGCGAACTGTACGGCAAACTGGTCACGCACGACGTGCCACATGTGG
ACAAATGCACCTTCTGCGTGCAGCGCCTGTCGGAAAAACTCGAACCGGCCTGCGTCGCCACCTGCCCGACCCACACGCGC
ATCTTCGGCGACCTCGATGACCGCAAGAGCGAGGTGCACAAGCTCGCCGCGAGCGGCAAGGCGCAGGCGCTCAACCAGGG
CCTCGGCACCTCGCCGAAAGTATTCTACATCCCATCATAA

Upstream 100 bases:

>100_bases
GATGCCGTACAGCATCGAGCCGATCGCAGCGTCGTTCATGTCGCAGGAGTTCACCGTAAGCGTCAAGCCTGTAAACAGTT
AACTGGAGGAGTAAGTATCT

Downstream 100 bases:

>100_bases
AGGAGGGCACTCATTATGACCTTTGTACATCAGGAAGTATGGCACTGGCAGATCGCAACCTACCTGTTTCTGGGCGGTCT
GGGCGGCGCGACGTTCGCCA

Product: polysulfide reductase, subunit B, putative

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MARYGMVMDMRTCVGCQACMAACSTENQTPFWSEKFRTHVEDKETGAFPDVRRVQLPRLCMHCENTPCLSACPTGATHMN
KDGIVLVNYDRCIGCYACCIACPYDARYAYDSEDVQKERELYGKLVTHDVPHVDKCTFCVQRLSEKLEPACVATCPTHTR
IFGDLDDRKSEVHKLAASGKAQALNQGLGTSPKVFYIPS

Sequences:

>Translated_199_residues
MARYGMVMDMRTCVGCQACMAACSTENQTPFWSEKFRTHVEDKETGAFPDVRRVQLPRLCMHCENTPCLSACPTGATHMN
KDGIVLVNYDRCIGCYACCIACPYDARYAYDSEDVQKERELYGKLVTHDVPHVDKCTFCVQRLSEKLEPACVATCPTHTR
IFGDLDDRKSEVHKLAASGKAQALNQGLGTSPKVFYIPS
>Mature_198_residues
ARYGMVMDMRTCVGCQACMAACSTENQTPFWSEKFRTHVEDKETGAFPDVRRVQLPRLCMHCENTPCLSACPTGATHMNK
DGIVLVNYDRCIGCYACCIACPYDARYAYDSEDVQKERELYGKLVTHDVPHVDKCTFCVQRLSEKLEPACVATCPTHTRI
FGDLDDRKSEVHKLAASGKAQALNQGLGTSPKVFYIPS

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=198, Percent_Identity=37.3737373737374, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI226510944, Length=171, Percent_Identity=35.672514619883, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787122, Length=205, Percent_Identity=32.1951219512195, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787872, Length=205, Percent_Identity=30.7317073170732, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787749, Length=201, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790326, Length=198, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1789370, Length=203, Percent_Identity=29.064039408867, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1789067, Length=187, Percent_Identity=32.0855614973262, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI87082179, Length=183, Percent_Identity=26.775956284153, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082180, Length=182, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082114, Length=202, Percent_Identity=31.6831683168317, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787740, Length=121, Percent_Identity=33.0578512396694, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1787478, Length=140, Percent_Identity=30, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789079, Length=108, Percent_Identity=37.037037037037, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI2367245, Length=186, Percent_Identity=28.494623655914, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22207; Mature: 22075

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
12.1 %Cys+Met (Translated Protein)
8.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
11.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYGMVMDMRTCVGCQACMAACSTENQTPFWSEKFRTHVEDKETGAFPDVRRVQLPRLC
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
MHCENTPCLSACPTGATHMNKDGIVLVNYDRCIGCYACCIACPYDARYAYDSEDVQKERE
HHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LYGKLVTHDVPHVDKCTFCVQRLSEKLEPACVATCPTHTRIFGDLDDRKSEVHKLAASGK
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHEECCCHHHHHHHHHHHHCCH
AQALNQGLGTSPKVFYIPS
HHHHHCCCCCCCEEEEECC
>Mature Secondary Structure 
ARYGMVMDMRTCVGCQACMAACSTENQTPFWSEKFRTHVEDKETGAFPDVRRVQLPRLC
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
MHCENTPCLSACPTGATHMNKDGIVLVNYDRCIGCYACCIACPYDARYAYDSEDVQKERE
HHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LYGKLVTHDVPHVDKCTFCVQRLSEKLEPACVATCPTHTRIFGDLDDRKSEVHKLAASGK
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHEECCCHHHHHHHHHHHHCCH
AQALNQGLGTSPKVFYIPS
HHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]