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The map label for this gene is mrpA [H]

Identifier: 21673319

GI number: 21673319

Start: 499482

End: 500891

Strand: Direct

Name: mrpA [H]

Synonym: CT0484

Alternate gene names: 21673319

Gene position: 499482-500891 (Clockwise)

Preceding gene: 21673318

Following gene: 21673320

Centisome position: 23.18

GC content: 60.0

Gene sequence:

>1410_bases
ATGAGGACCCCGCCTTTGCTTGCCCTGATCATCACCGGCTTTGCCGTTTCGGCAGCTGCTCCATATCTCTATCGTTTGCT
GAAGGCCCGTTTTGTCTGGTTCGGAGTTGCCTTTCCGCTAGCTCTGTTCGCGAGCTTCATGCTCCGCTATCCGCAGGCGG
CCTCCGGCGTGCCGGTGCGCGAGCGCTGGAGCTGGGTGCCGTCGCTCGGTCTTGACCTGAGCTTCGTGCTCGACGGGCTG
AGCCTGACCTTCGTGATGCTGGTGACGCTTATCGGTGCGGCGGTGTTTCTGTACGCTTCGGTCTACCTGCGCCACCATGA
AGAGGCCGACCGCTTTTTTGGCTTCATTGGCATGTTCATGACCTGGATGCTCGGCGTGGTACTGGCCGATAACATGCTGC
TGCTGTTTCTCTTCTGGGAGCTGACCAGCATCAGCTCGTTCCTCCTGATCGGCTTCAACCACCATGCGGCTTCGTCGCGC
GCTTCAGCGCTGAAGGCGCTGCTCGTGACCGGGGCGGGCGGCCTCCTGGCGCTGTTGGCCGGAATGCTGCTGTTGGGGAA
CGTGACCGGCAGCTTCGAGATTTCCTCGTTCTACGCGATGAACGATCTCATCACCTCGCATCGCCTCTATCCGGCCATTG
TCGCGCTGATTCTTGTCGGCGCGTTCACCCAATCGGTGCAGTTTCCGTTCCACTTCTGGCTTCCCGACGCCATGGCCGCG
CCTTCGCCGGTGAGCGCCTACCTGCACTCGGCCACGATGGTCAAGGCGGGCATCTATCTCATCGCGCGGTTCAACCACGA
AATCGGCAGCACCGCGCTCTGGCAGGATACGATCCTCTTTACCGGCGCAGCCACCATGATTTTCGCAGGCTTGCTCTTCT
ACCGCCAGAGCGACCTGAAAAGGTTGCTGCCACTTCATCTGCCTGGTCTCGTCAAGCCATCGATCTGGTACGAAGAGGCG
CTGTCGGGGATGCTGCGGTTTGCCGCCTGGCTCACCTCGGTGTTGCAGAACGGCGATTTGCGACGCTATCTCGCGGTCAT
CATCTTCTCGGCGCTCATTCCGGTTTCACTGATGCTTTTCAGCTCTGGCGGTTTTTCGGTAACGCTGCCCGCCGATCTTT
CGGTCGCGTCGTATGAGGTCGCGCTTGCCCTGATTATAGTGCTGGCGACCGCCCTCTTGCTAACGAGCGATTCGCGCCTG
AAGGCGATTGTGTCGATGGGCGTGCTCGGCTTTGGTGTTGGTATGATCTTCATCATCTACGGCGCGCCCGACGTGGCGCT
GACCACTTTTGTTGCCATCGAGACGCTGAACGTCATTCTCTTTGTGCTGGTGCTGGCCCATCTGCCGAAGTTCACCTCTC
GTTCGCGCACCACCGGGCGGATTCGCGCTGCTCAAACTCAGAACCGGTAA

Upstream 100 bases:

>100_bases
CGTTAAGAGGAACCTCTTGTCATAAAACGAGGTTGCTCTGAGCAGAACGTTGATTCGTCTAATAGAAGTTGATGGCCTGC
AACCGGGCCTTCGACATTTG

Downstream 100 bases:

>100_bases
AACGGATTGTTAGGCGGACAATCACAAATCATAAGGAGATAAACATGAAAGAGTACAAAGTTCTCACACAGAAGGACCGC
TTCTTCGGCGGCACGTTCGA

Product: NADH dehydrogenase I

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRTPPLLALIITGFAVSAAAPYLYRLLKARFVWFGVAFPLALFASFMLRYPQAASGVPVRERWSWVPSLGLDLSFVLDGL
SLTFVMLVTLIGAAVFLYASVYLRHHEEADRFFGFIGMFMTWMLGVVLADNMLLLFLFWELTSISSFLLIGFNHHAASSR
ASALKALLVTGAGGLLALLAGMLLLGNVTGSFEISSFYAMNDLITSHRLYPAIVALILVGAFTQSVQFPFHFWLPDAMAA
PSPVSAYLHSATMVKAGIYLIARFNHEIGSTALWQDTILFTGAATMIFAGLLFYRQSDLKRLLPLHLPGLVKPSIWYEEA
LSGMLRFAAWLTSVLQNGDLRRYLAVIIFSALIPVSLMLFSSGGFSVTLPADLSVASYEVALALIIVLATALLLTSDSRL
KAIVSMGVLGFGVGMIFIIYGAPDVALTTFVAIETLNVILFVLVLAHLPKFTSRSRTTGRIRAAQTQNR

Sequences:

>Translated_469_residues
MRTPPLLALIITGFAVSAAAPYLYRLLKARFVWFGVAFPLALFASFMLRYPQAASGVPVRERWSWVPSLGLDLSFVLDGL
SLTFVMLVTLIGAAVFLYASVYLRHHEEADRFFGFIGMFMTWMLGVVLADNMLLLFLFWELTSISSFLLIGFNHHAASSR
ASALKALLVTGAGGLLALLAGMLLLGNVTGSFEISSFYAMNDLITSHRLYPAIVALILVGAFTQSVQFPFHFWLPDAMAA
PSPVSAYLHSATMVKAGIYLIARFNHEIGSTALWQDTILFTGAATMIFAGLLFYRQSDLKRLLPLHLPGLVKPSIWYEEA
LSGMLRFAAWLTSVLQNGDLRRYLAVIIFSALIPVSLMLFSSGGFSVTLPADLSVASYEVALALIIVLATALLLTSDSRL
KAIVSMGVLGFGVGMIFIIYGAPDVALTTFVAIETLNVILFVLVLAHLPKFTSRSRTTGRIRAAQTQNR
>Mature_469_residues
MRTPPLLALIITGFAVSAAAPYLYRLLKARFVWFGVAFPLALFASFMLRYPQAASGVPVRERWSWVPSLGLDLSFVLDGL
SLTFVMLVTLIGAAVFLYASVYLRHHEEADRFFGFIGMFMTWMLGVVLADNMLLLFLFWELTSISSFLLIGFNHHAASSR
ASALKALLVTGAGGLLALLAGMLLLGNVTGSFEISSFYAMNDLITSHRLYPAIVALILVGAFTQSVQFPFHFWLPDAMAA
PSPVSAYLHSATMVKAGIYLIARFNHEIGSTALWQDTILFTGAATMIFAGLLFYRQSDLKRLLPLHLPGLVKPSIWYEEA
LSGMLRFAAWLTSVLQNGDLRRYLAVIIFSALIPVSLMLFSSGGFSVTLPADLSVASYEVALALIIVLATALLLTSDSRL
KAIVSMGVLGFGVGMIFIIYGAPDVALTTFVAIETLNVILFVLVLAHLPKFTSRSRTTGRIRAAQTQNR

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=304, Percent_Identity=28.9473684210526, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1788614, Length=248, Percent_Identity=38.7096774193548, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788829, Length=242, Percent_Identity=36.3636363636364, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788613, Length=259, Percent_Identity=30.1158301158301, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788831, Length=247, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788827, Length=239, Percent_Identity=30.9623430962343, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 51228; Mature: 51228

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPPLLALIITGFAVSAAAPYLYRLLKARFVWFGVAFPLALFASFMLRYPQAASGVPVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
ERWSWVPSLGLDLSFVLDGLSLTFVMLVTLIGAAVFLYASVYLRHHEEADRFFGFIGMFM
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TWMLGVVLADNMLLLFLFWELTSISSFLLIGFNHHAASSRASALKALLVTGAGGLLALLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GMLLLGNVTGSFEISSFYAMNDLITSHRLYPAIVALILVGAFTQSVQFPFHFWLPDAMAA
HHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCC
PSPVSAYLHSATMVKAGIYLIARFNHEIGSTALWQDTILFTGAATMIFAGLLFYRQSDLK
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLPLHLPGLVKPSIWYEEALSGMLRFAAWLTSVLQNGDLRRYLAVIIFSALIPVSLMLF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SSGGFSVTLPADLSVASYEVALALIIVLATALLLTSDSRLKAIVSMGVLGFGVGMIFIIY
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEE
GAPDVALTTFVAIETLNVILFVLVLAHLPKFTSRSRTTGRIRAAQTQNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MRTPPLLALIITGFAVSAAAPYLYRLLKARFVWFGVAFPLALFASFMLRYPQAASGVPVR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
ERWSWVPSLGLDLSFVLDGLSLTFVMLVTLIGAAVFLYASVYLRHHEEADRFFGFIGMFM
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TWMLGVVLADNMLLLFLFWELTSISSFLLIGFNHHAASSRASALKALLVTGAGGLLALLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GMLLLGNVTGSFEISSFYAMNDLITSHRLYPAIVALILVGAFTQSVQFPFHFWLPDAMAA
HHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCC
PSPVSAYLHSATMVKAGIYLIARFNHEIGSTALWQDTILFTGAATMIFAGLLFYRQSDLK
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLPLHLPGLVKPSIWYEEALSGMLRFAAWLTSVLQNGDLRRYLAVIIFSALIPVSLMLF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SSGGFSVTLPADLSVASYEVALALIIVLATALLLTSDSRLKAIVSMGVLGFGVGMIFIIY
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEE
GAPDVALTTFVAIETLNVILFVLVLAHLPKFTSRSRTTGRIRAAQTQNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]