Click here to switch to the map view.

The map label for this gene is cobA [H]

Identifier: 21673229

GI number: 21673229

Start: 404925

End: 405728

Strand: Reverse

Name: cobA [H]

Synonym: CT0390

Alternate gene names: 21673229

Gene position: 405728-404925 (Counterclockwise)

Preceding gene: 21673230

Following gene: 21673228

Centisome position: 18.83

GC content: 64.55

Gene sequence:

>804_bases
ATGAGCGACGGTAAGGGTAAGGTTTTTCTGGTGGGCGGCGGGCCGGGCGATCCGGAGCTGCTGACCATACGTGCACACAA
CGTGCTGCAATCGGCGGACGTGGTGCTGCATGACGCGCTCATCAGCCCGGAAATTCTTGCGTTGCTGCCGAACGGCGCGG
AGCGGATCAGCGTCGGCAAGCGCCTCGGCGACGGCAAGGATCAGACCGACCGCCAGACGAAGATCAACGACCTGCTCGTC
CGTCACGCCAGAGAGGGTAAATGCGTCGTACGGCTGAAGGCTGGCGACCCCTTCATGTTCGGGCGCGGCATCGAGGAGGT
GCGTGCGCTCGCCGCCGCCGGAGTGCCGTGCGAGGTGGTGCCGGGCATCACGACGGGCATCGCCGCCGCTGACCTCTGCG
GCATCCCGCTCACCGAACGCCACCGGAACAGCTCGGTGCTCTTCTGCACCGGCCACACGGCGGACTACTCGCTTGGGCAT
TTCGCGGCGGTGATCGAGCTGATGAAGGCGGGTACGCCGCTGGTGATGTACATGGGTTTCGAGAATCTGGACAAGATCGT
CGAGCGCTTCATCGACTCCGGGCTGTCGCCGGAACTCCCGGCGTGCGCGGTTTCGCGCGTCTCGCGGAGCGACCAGACGC
TGGTTGCGGCCACGATCGGCACGATTGTCCAGCAGATTCGCGAGCGCGAGCTATCCCTGCCGGTGGTGTTCATCATCGGC
GAACACGCCGTTCCCGAGGGGGCGTGCCCAGATCAGTCCGATGCGTCCGATCCGTCTGATCAAAATCACAATGAACAGCA
ATGA

Upstream 100 bases:

>100_bases
ATTCTCGTCTCGAAGGACGGCTTCACGCTGCCTGGCAATGTGATGACAATTGGCGCAGCGCGAAAGGTTTTGAAAACATT
GAAAGAGAATCAAAAAAGCG

Downstream 100 bases:

>100_bases
GTAAGCACAAGAAACGGTCACAGATCAACAAGAAGGCGATTCTGCTTGCCCATTTCGGCACCACCTATCCCTCGGCACTG
CCGTCGCTGGAGAACATCCG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSDGKGKVFLVGGGPGDPELLTIRAHNVLQSADVVLHDALISPEILALLPNGAERISVGKRLGDGKDQTDRQTKINDLLV
RHAREGKCVVRLKAGDPFMFGRGIEEVRALAAAGVPCEVVPGITTGIAAADLCGIPLTERHRNSSVLFCTGHTADYSLGH
FAAVIELMKAGTPLVMYMGFENLDKIVERFIDSGLSPELPACAVSRVSRSDQTLVAATIGTIVQQIRERELSLPVVFIIG
EHAVPEGACPDQSDASDPSDQNHNEQQ

Sequences:

>Translated_267_residues
MSDGKGKVFLVGGGPGDPELLTIRAHNVLQSADVVLHDALISPEILALLPNGAERISVGKRLGDGKDQTDRQTKINDLLV
RHAREGKCVVRLKAGDPFMFGRGIEEVRALAAAGVPCEVVPGITTGIAAADLCGIPLTERHRNSSVLFCTGHTADYSLGH
FAAVIELMKAGTPLVMYMGFENLDKIVERFIDSGLSPELPACAVSRVSRSDQTLVAATIGTIVQQIRERELSLPVVFIIG
EHAVPEGACPDQSDASDPSDQNHNEQQ
>Mature_266_residues
SDGKGKVFLVGGGPGDPELLTIRAHNVLQSADVVLHDALISPEILALLPNGAERISVGKRLGDGKDQTDRQTKINDLLVR
HAREGKCVVRLKAGDPFMFGRGIEEVRALAAAGVPCEVVPGITTGIAAADLCGIPLTERHRNSSVLFCTGHTADYSLGHF
AAVIELMKAGTPLVMYMGFENLDKIVERFIDSGLSPELPACAVSRVSRSDQTLVAATIGTIVQQIRERELSLPVVFIIGE
HAVPEGACPDQSDASDPSDQNHNEQQ

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=241, Percent_Identity=39.8340248962656, Blast_Score=153, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322922, Length=236, Percent_Identity=30.9322033898305, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 28462; Mature: 28331

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGKGKVFLVGGGPGDPELLTIRAHNVLQSADVVLHDALISPEILALLPNGAERISVGK
CCCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
RLGDGKDQTDRQTKINDLLVRHAREGKCVVRLKAGDPFMFGRGIEEVRALAAAGVPCEVV
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCEEC
PGITTGIAAADLCGIPLTERHRNSSVLFCTGHTADYSLGHFAAVIELMKAGTPLVMYMGF
CCCHHHHHHHHHCCCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECH
ENLDKIVERFIDSGLSPELPACAVSRVSRSDQTLVAATIGTIVQQIRERELSLPVVFIIG
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EHAVPEGACPDQSDASDPSDQNHNEQQ
CCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDGKGKVFLVGGGPGDPELLTIRAHNVLQSADVVLHDALISPEILALLPNGAERISVGK
CCCCEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
RLGDGKDQTDRQTKINDLLVRHAREGKCVVRLKAGDPFMFGRGIEEVRALAAAGVPCEVV
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCEEC
PGITTGIAAADLCGIPLTERHRNSSVLFCTGHTADYSLGHFAAVIELMKAGTPLVMYMGF
CCCHHHHHHHHHCCCCCHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECH
ENLDKIVERFIDSGLSPELPACAVSRVSRSDQTLVAATIGTIVQQIRERELSLPVVFIIG
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EHAVPEGACPDQSDASDPSDQNHNEQQ
CCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA