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The map label for this gene is cbiL [H]

Identifier: 21673227

GI number: 21673227

Start: 403221

End: 403961

Strand: Reverse

Name: cbiL [H]

Synonym: CT0388

Alternate gene names: 21673227

Gene position: 403961-403221 (Counterclockwise)

Preceding gene: 21673228

Following gene: 21673226

Centisome position: 18.75

GC content: 62.08

Gene sequence:

>741_bases
ATGAACAATCAAGGCTCGATCATCAGCGTTTCGCTTGGACCGGGTGATCCCGGGCTGATTACCGTCAAGGCGCTTTCGCA
GCTTCGCGAGGCTGACGTGATCTACTATCCCGGCACGGTCAGCGCTTCGGGCGCGGTGACGAGCGTGGCGCTCGATATTC
TCAAGGAGTTCGATCTCGATCCGTCGAAGCTCCGGGGAATGCTCGTGCCGATGTCGCGCTCGCGTGGGGCCGCCGAGGCG
AGTTATGCCGCGAACTACGCCTCGATGGCGGAAGAAGTGCAGGCGGGCCGACGGGTGGCGGTGGTGAGTGTCGGTGACGG
CGGCTTTTACAGCACTGCGTCGGCGATTATCGAACGGGCGCGGCGAGACGGTCTCGATTGCTCGATGACGCCAGGAATTC
CGGCCTTCATCGCTGCCGGGTCAGCTGCCGGGATGCCGCTTGCGTTGCAGAGCGACAGCGTGTTGGTGTTGGCGCAGATC
GACGAGATCGGCGAACTCGAACGCGCTCTCGTCACGCACAGCACCGTAGTGGTGATGAAGCTCTCGACGGTCAGAGACGA
GCTGGTTAGCTTCCTCGAACGGTACGCCAAACCCTTCCTCTACGCCGAAAAGGTGGGCATGGCGGGCGAGTTCATCACGA
TGGAAGTCGACGCCTTGCGCTCACGCGCTATCCCCTACTTTTCGCTGCTGGTCTGCTCGCCGCATTGCCGGCAGTCAACC
CTTTCGCCCTTTGCGTCATGA

Upstream 100 bases:

>100_bases
TTCGCCAAGCGGATTCTTGAAACCGCTGAAGATCATGGTATCGATCTGTTTTCAGCATCCGACAAAATCGATTAACGCTT
TGAACAACCGTTTGCAACCA

Downstream 100 bases:

>100_bases
ACGGCACGATTACCGTTGTGGGCCTCGGCCCCGGCAGCGACTCGATGATGACCCCGCAGGTGCTCGATGCGATCCGCACG
GCTGACGCGGTGGTCGGCTA

Product: precorrin-2 C20-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNNQGSIISVSLGPGDPGLITVKALSQLREADVIYYPGTVSASGAVTSVALDILKEFDLDPSKLRGMLVPMSRSRGAAEA
SYAANYASMAEEVQAGRRVAVVSVGDGGFYSTASAIIERARRDGLDCSMTPGIPAFIAAGSAAGMPLALQSDSVLVLAQI
DEIGELERALVTHSTVVVMKLSTVRDELVSFLERYAKPFLYAEKVGMAGEFITMEVDALRSRAIPYFSLLVCSPHCRQST
LSPFAS

Sequences:

>Translated_246_residues
MNNQGSIISVSLGPGDPGLITVKALSQLREADVIYYPGTVSASGAVTSVALDILKEFDLDPSKLRGMLVPMSRSRGAAEA
SYAANYASMAEEVQAGRRVAVVSVGDGGFYSTASAIIERARRDGLDCSMTPGIPAFIAAGSAAGMPLALQSDSVLVLAQI
DEIGELERALVTHSTVVVMKLSTVRDELVSFLERYAKPFLYAEKVGMAGEFITMEVDALRSRAIPYFSLLVCSPHCRQST
LSPFAS
>Mature_246_residues
MNNQGSIISVSLGPGDPGLITVKALSQLREADVIYYPGTVSASGAVTSVALDILKEFDLDPSKLRGMLVPMSRSRGAAEA
SYAANYASMAEEVQAGRRVAVVSVGDGGFYSTASAIIERARRDGLDCSMTPGIPAFIAAGSAAGMPLALQSDSVLVLAQI
DEIGELERALVTHSTVVVMKLSTVRDELVSFLERYAKPFLYAEKVGMAGEFITMEVDALRSRAIPYFSLLVCSPHCRQST
LSPFAS

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 26046; Mature: 26046

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQGSIISVSLGPGDPGLITVKALSQLREADVIYYPGTVSASGAVTSVALDILKEFDLD
CCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCC
PSKLRGMLVPMSRSRGAAEASYAANYASMAEEVQAGRRVAVVSVGDGGFYSTASAIIERA
HHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
RRDGLDCSMTPGIPAFIAAGSAAGMPLALQSDSVLVLAQIDEIGELERALVTHSTVVVMK
HHCCCCCCCCCCCHHHEECCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHCEEEEEE
LSTVRDELVSFLERYAKPFLYAEKVGMAGEFITMEVDALRSRAIPYFSLLVCSPHCRQST
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEHHHHHHCCCCEEEEEEECCCHHHHC
LSPFAS
CCCCCC
>Mature Secondary Structure
MNNQGSIISVSLGPGDPGLITVKALSQLREADVIYYPGTVSASGAVTSVALDILKEFDLD
CCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCC
PSKLRGMLVPMSRSRGAAEASYAANYASMAEEVQAGRRVAVVSVGDGGFYSTASAIIERA
HHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
RRDGLDCSMTPGIPAFIAAGSAAGMPLALQSDSVLVLAQIDEIGELERALVTHSTVVVMK
HHCCCCCCCCCCCHHHEECCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHCEEEEEE
LSTVRDELVSFLERYAKPFLYAEKVGMAGEFITMEVDALRSRAIPYFSLLVCSPHCRQST
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEHHHHHHCCCCEEEEEEECCCHHHHC
LSPFAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]