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The map label for this gene is cbiGF [H]

Identifier: 21673224

GI number: 21673224

Start: 398680

End: 400554

Strand: Reverse

Name: cbiGF [H]

Synonym: CT0385

Alternate gene names: 21673224

Gene position: 400554-398680 (Counterclockwise)

Preceding gene: 21673225

Following gene: 21673223

Centisome position: 18.59

GC content: 64.43

Gene sequence:

>1875_bases
ATGCACGAACGAATTGCCATTATCGCCATCACCGAAACCGGAATCGCGCTCGCCCGCTCACTGAAAAGCCTGCTCGTGGC
CGACGGGTTTGCCGGGTGCGGGCTGTTCTCGTCCCGCGTCGCCGAGGGTGTCGAACCCATCGAAAGCGTTCCGGCGTTTG
TCCGGCACTCGTTCGGCAGCTTCGACGCTTTTCTTTTTATAGGCTCGCTCGGCATCTGCGTGCGCTCGATTGCGCCGGTG
TTGCAGGGCAAGCTGCGCGACCCGGCGGTCATCAACTGCGACGAGTCCGGGCGCTTCGTGCAGAGCGTGTTGTCGGGCCA
TGCGGGCGGGGCGAACGCGCTGGCCGGGCGGGTCGCGCGGCTGCTGGGGGCACAGGCGGTGCTGAGCACGTCGAGCGACG
TGCAGGGGTTGTGGCCGCTCGACATTCTGGGGCGTGAAGAGGGGTGGGGCGTCGAGTTCGCCTCGCCGGTCGCGGGTGAA
TCGATGACTACGGCGATGGCGGCCTTCGTGAACCACGAGCCGACCACGCTGCTACTTGATGTTCGCGACTCGCTGACCGA
TGAGTTGGAGCGAACCGCACCGCCGTTCGTGACGATTGCCTATCGCTACGAAGAGGTCGATTTCAGCACGTGCAGTCTTC
TGCTCGCCGTGACGCCGCGGATCATCGAAGCATCTGTCCAGACGGTGTTCTATCGACCGAAGGTGCTCTGCGTTGGCGTT
GGGTCGGAGCGAGGCATCGACCCCGAACGGTTCGTCAGCTCGATTTCCGAACAATTTGCAAGCAACGGTTTCTCCATGCG
CTCGATCCGCAGCGTCGGTTCGGTTGATTTCAAGCTAAACGAAGAGGCCTTCATTGCCTTTGCCGAAGCGTGCGGCACGA
CGCTGACGGGTTTCACGCCGGAGCAGCTCGAAAGTGTCGGGCCGGTACCCAATCCGTCTGATGTCGTCTTCCGCAAAACT
GGCGTGCACAGCGTTTCCGAGGCTTCGGCGGCGCTGCTCTCCGGCGAGAACCGCTGGCTGATCGAGAAACAGAAGATCTC
GCTCGACGGTATTCCGGAGGGAGAGCCGCGCCACTACACCTTCGCCGTCAGCCTTTTGCGCGGAGCTGAACGGCGCGGGC
GCATCGCCATCGTCGGGGCGGGGCCGGGCGATCCGGAGCTGGTGACGGTCAAGGGCAAACGCTACCTCGAACAGGCCGAC
CTGATTCTCTACGCGGGCAGCCTCGTGCCGGAGAAGCTGACCCACTATGCCAAACCGGGCGCGCTGGTGCGGAGTTCGGC
TTCGCTTTCGCTCGAAGAGCAGTTCGCGTTGATGGAGCGGTTCTATCGACAGGGCAAATTCGTGGTGCGACTGCACACCG
GCGATCCGTCGATTTACGGAGCGATTCAGGAGCAGATGGCCTTTTTCGACGCTGAGGGGTTCGAGTACGAAATCGTGCCG
GGCGTGTCGTCGTTCCAGGCAGCGGCAGCGGTGTTGCAGTCGCAGTTTACCGTGCCGGAGAAGGTGCAGACCATCATCCT
GACGCGCGGCAGCGGGCGGACGCCGGTGCCAGACAAGGAGCGGCTCTCGGAGCTGGCGCGTGCGAGGGCGACCATGTGCA
TCTACCTCAGCGCCGAGTGGAGCGACCAGGTGCAGTCCGAGCTGCTCGAACACTACGCGCCCGATACCCCCGTGGCGGTC
TGCTACCGGCTCACCTGGGACGACCAGCAGGTGTGGCGCGGGCGGCTCGACGGGCTGGCCTCGCTGGTGCGCGAAAGCGG
CAAAACCCGCACGGTGCTGCTGGTGGTCGGTGAGGCGATCGGTGCGCGCGGCGGGCGCTCGAAGCTCTACGACCCATCCT
TCACGCATGGCTTCCGCGAAGGGCATGGCGCGTGA

Upstream 100 bases:

>100_bases
TGAGTGTGATGAAGGCGGTGATGCCGGGCTGAGCGCTTGAGACGCCCTGTCCGATCAGAACGTTATCCGGACTATTTCAA
ACAGTTGTTTTACAAAACCC

Downstream 100 bases:

>100_bases
GGAGGGCGGCGTGATTCTGCTCTTCGGCGGTACCACCGAGGGACGGCAGGCGGCGGCGCTCTGCGACCGGCTCGGCCTGC
CTTTCATCTACTCGACCAAA

Product: precorrin-4 C11-methyltransferase/cobalamin biosynthesis protein

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MHERIAIIAITETGIALARSLKSLLVADGFAGCGLFSSRVAEGVEPIESVPAFVRHSFGSFDAFLFIGSLGICVRSIAPV
LQGKLRDPAVINCDESGRFVQSVLSGHAGGANALAGRVARLLGAQAVLSTSSDVQGLWPLDILGREEGWGVEFASPVAGE
SMTTAMAAFVNHEPTTLLLDVRDSLTDELERTAPPFVTIAYRYEEVDFSTCSLLLAVTPRIIEASVQTVFYRPKVLCVGV
GSERGIDPERFVSSISEQFASNGFSMRSIRSVGSVDFKLNEEAFIAFAEACGTTLTGFTPEQLESVGPVPNPSDVVFRKT
GVHSVSEASAALLSGENRWLIEKQKISLDGIPEGEPRHYTFAVSLLRGAERRGRIAIVGAGPGDPELVTVKGKRYLEQAD
LILYAGSLVPEKLTHYAKPGALVRSSASLSLEEQFALMERFYRQGKFVVRLHTGDPSIYGAIQEQMAFFDAEGFEYEIVP
GVSSFQAAAAVLQSQFTVPEKVQTIILTRGSGRTPVPDKERLSELARARATMCIYLSAEWSDQVQSELLEHYAPDTPVAV
CYRLTWDDQQVWRGRLDGLASLVRESGKTRTVLLVVGEAIGARGGRSKLYDPSFTHGFREGHGA

Sequences:

>Translated_624_residues
MHERIAIIAITETGIALARSLKSLLVADGFAGCGLFSSRVAEGVEPIESVPAFVRHSFGSFDAFLFIGSLGICVRSIAPV
LQGKLRDPAVINCDESGRFVQSVLSGHAGGANALAGRVARLLGAQAVLSTSSDVQGLWPLDILGREEGWGVEFASPVAGE
SMTTAMAAFVNHEPTTLLLDVRDSLTDELERTAPPFVTIAYRYEEVDFSTCSLLLAVTPRIIEASVQTVFYRPKVLCVGV
GSERGIDPERFVSSISEQFASNGFSMRSIRSVGSVDFKLNEEAFIAFAEACGTTLTGFTPEQLESVGPVPNPSDVVFRKT
GVHSVSEASAALLSGENRWLIEKQKISLDGIPEGEPRHYTFAVSLLRGAERRGRIAIVGAGPGDPELVTVKGKRYLEQAD
LILYAGSLVPEKLTHYAKPGALVRSSASLSLEEQFALMERFYRQGKFVVRLHTGDPSIYGAIQEQMAFFDAEGFEYEIVP
GVSSFQAAAAVLQSQFTVPEKVQTIILTRGSGRTPVPDKERLSELARARATMCIYLSAEWSDQVQSELLEHYAPDTPVAV
CYRLTWDDQQVWRGRLDGLASLVRESGKTRTVLLVVGEAIGARGGRSKLYDPSFTHGFREGHGA
>Mature_624_residues
MHERIAIIAITETGIALARSLKSLLVADGFAGCGLFSSRVAEGVEPIESVPAFVRHSFGSFDAFLFIGSLGICVRSIAPV
LQGKLRDPAVINCDESGRFVQSVLSGHAGGANALAGRVARLLGAQAVLSTSSDVQGLWPLDILGREEGWGVEFASPVAGE
SMTTAMAAFVNHEPTTLLLDVRDSLTDELERTAPPFVTIAYRYEEVDFSTCSLLLAVTPRIIEASVQTVFYRPKVLCVGV
GSERGIDPERFVSSISEQFASNGFSMRSIRSVGSVDFKLNEEAFIAFAEACGTTLTGFTPEQLESVGPVPNPSDVVFRKT
GVHSVSEASAALLSGENRWLIEKQKISLDGIPEGEPRHYTFAVSLLRGAERRGRIAIVGAGPGDPELVTVKGKRYLEQAD
LILYAGSLVPEKLTHYAKPGALVRSSASLSLEEQFALMERFYRQGKFVVRLHTGDPSIYGAIQEQMAFFDAEGFEYEIVP
GVSSFQAAAAVLQSQFTVPEKVQTIILTRGSGRTPVPDKERLSELARARATMCIYLSAEWSDQVQSELLEHYAPDTPVAV
CYRLTWDDQQVWRGRLDGLASLVRESGKTRTVLLVVGEAIGARGGRSKLYDPSFTHGFREGHGA

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=241, Percent_Identity=29.045643153527, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6322922, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 67531; Mature: 67531

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHERIAIIAITETGIALARSLKSLLVADGFAGCGLFSSRVAEGVEPIESVPAFVRHSFGS
CCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCC
FDAFLFIGSLGICVRSIAPVLQGKLRDPAVINCDESGRFVQSVLSGHAGGANALAGRVAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHHH
LLGAQAVLSTSSDVQGLWPLDILGREEGWGVEFASPVAGESMTTAMAAFVNHEPTTLLLD
HHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEE
VRDSLTDELERTAPPFVTIAYRYEEVDFSTCSLLLAVTPRIIEASVQTVFYRPKVLCVGV
CHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHCHHHHHHHHHHHEECCEEEEEEC
GSERGIDPERFVSSISEQFASNGFSMRSIRSVGSVDFKLNEEAFIAFAEACGTTLTGFTP
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCH
EQLESVGPVPNPSDVVFRKTGVHSVSEASAALLSGENRWLIEKQKISLDGIPEGEPRHYT
HHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEH
FAVSLLRGAERRGRIAIVGAGPGDPELVTVKGKRYLEQADLILYAGSLVPEKLTHYAKPG
HHHHHHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCHHHHHHHCCCC
ALVRSSASLSLEEQFALMERFYRQGKFVVRLHTGDPSIYGAIQEQMAFFDAEGFEYEIVP
HHEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEC
GVSSFQAAAAVLQSQFTVPEKVQTIILTRGSGRTPVPDKERLSELARARATMCIYLSAEW
CCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCEEEEEEECCC
SDQVQSELLEHYAPDTPVAVCYRLTWDDQQVWRGRLDGLASLVRESGKTRTVLLVVGEAI
HHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHH
GARGGRSKLYDPSFTHGFREGHGA
CCCCCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MHERIAIIAITETGIALARSLKSLLVADGFAGCGLFSSRVAEGVEPIESVPAFVRHSFGS
CCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCC
FDAFLFIGSLGICVRSIAPVLQGKLRDPAVINCDESGRFVQSVLSGHAGGANALAGRVAR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHHH
LLGAQAVLSTSSDVQGLWPLDILGREEGWGVEFASPVAGESMTTAMAAFVNHEPTTLLLD
HHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEE
VRDSLTDELERTAPPFVTIAYRYEEVDFSTCSLLLAVTPRIIEASVQTVFYRPKVLCVGV
CHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHCHHHHHHHHHHHEECCEEEEEEC
GSERGIDPERFVSSISEQFASNGFSMRSIRSVGSVDFKLNEEAFIAFAEACGTTLTGFTP
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCH
EQLESVGPVPNPSDVVFRKTGVHSVSEASAALLSGENRWLIEKQKISLDGIPEGEPRHYT
HHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEH
FAVSLLRGAERRGRIAIVGAGPGDPELVTVKGKRYLEQADLILYAGSLVPEKLTHYAKPG
HHHHHHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCEEEEECCCCHHHHHHHCCCC
ALVRSSASLSLEEQFALMERFYRQGKFVVRLHTGDPSIYGAIQEQMAFFDAEGFEYEIVP
HHEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEC
GVSSFQAAAAVLQSQFTVPEKVQTIILTRGSGRTPVPDKERLSELARARATMCIYLSAEW
CCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCEEEEEEECCC
SDQVQSELLEHYAPDTPVAVCYRLTWDDQQVWRGRLDGLASLVRESGKTRTVLLVVGEAI
HHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHH
GARGGRSKLYDPSFTHGFREGHGA
CCCCCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]