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The map label for this gene is nfo

Identifier: 21673155

GI number: 21673155

Start: 332294

End: 333139

Strand: Reverse

Name: nfo

Synonym: CT0316

Alternate gene names: 21673155

Gene position: 333139-332294 (Counterclockwise)

Preceding gene: 21673156

Following gene: 21673152

Centisome position: 15.46

GC content: 59.69

Gene sequence:

>846_bases
ATGAAACGGGTCGGCGCCCATGTCAGTATTGCCGGAGGCGTCGAGAACGCCCCGCTGAACGCACAAAAGATCGGCGCGAA
AGCGTTTGCCATGTTCACCCGCAACCAGCGCCAGTGGCACTCCGCGCCGCTTACGGCGGCCTCGATCGAGGCGTTCCGGC
GCAATTGCGATGAAGCAGGATTCCTGCCTGAGCATATTTTGCCGCACGACAGCTACCTCATCAACCTCGGTGCTCCCGAA
GCCGACAAGATCGAAAAGTCGCGCAAGGCGTTCGTCACCGAAATGCAGCGCGCCGAGGCGCTCGGCCTGACGATGCTGAA
CTTCCACCCCGGCAGCCACCTCAATCTGACTGATGAAGATGCCTGCCTCAAAACCATCGCCGAATCGGTGAACCGCTCAC
TCGATGCGACGGCGGGTGTGACAGCGGTGATCGAAAACACGGCAGGACAGGGCAGTAATCTCGGCTGGCGCTTCGAGCAT
CTGGCGCGAATCATCGAACTGGTGGAGGACAAGTCGCGCGTCGGCGTCTGCCTCGACACCTGCCATCTTTTCGCCAGTGG
CTACGATTTGAGAACGCCGGAAGCATTCGACGCGACGCTGAGAGAGTTTGACCGTGTTGTGGGATTGCTTTATCTGAAGG
GAATGCACCTGAACGATGCGAAGCAGAAACTCGGCAGTAAGGTTGACCGGCACGAGTGCCTCGGCAAGGGGATGATCGGC
ATCGACGCCTTTGCGCACATCATGCGTCACCCGGCGCTCGAGGAGATACCGCTGATACTCGAAACCCCCAATGCCGAGGG
CTGGGCCGAAGAGATCGCGATGCTCTATGGCTTCACCAACGAGTAG

Upstream 100 bases:

>100_bases
GCCGGACGAGATCATCGACAGGCTAACCGATTGAACCTTCAACGGCAGATCGTCGTTAAGCTCTGATCAACACACGAAAA
CCTGCAAACAACACGATAGT

Downstream 100 bases:

>100_bases
CCATTTATCCCGGCGGCAGCGCTCAGGAAACGCGCAGGAAGCGGGCCACCGACCAGGTGCTGCCGAAAAAACCGAGCACA
ATGCCGAGCAGGACAAGTCC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLTAASIEAFRRNCDEAGFLPEHILPHDSYLINLGAPE
ADKIEKSRKAFVTEMQRAEALGLTMLNFHPGSHLNLTDEDACLKTIAESVNRSLDATAGVTAVIENTAGQGSNLGWRFEH
LARIIELVEDKSRVGVCLDTCHLFASGYDLRTPEAFDATLREFDRVVGLLYLKGMHLNDAKQKLGSKVDRHECLGKGMIG
IDAFAHIMRHPALEEIPLILETPNAEGWAEEIAMLYGFTNE

Sequences:

>Translated_281_residues
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLTAASIEAFRRNCDEAGFLPEHILPHDSYLINLGAPE
ADKIEKSRKAFVTEMQRAEALGLTMLNFHPGSHLNLTDEDACLKTIAESVNRSLDATAGVTAVIENTAGQGSNLGWRFEH
LARIIELVEDKSRVGVCLDTCHLFASGYDLRTPEAFDATLREFDRVVGLLYLKGMHLNDAKQKLGSKVDRHECLGKGMIG
IDAFAHIMRHPALEEIPLILETPNAEGWAEEIAMLYGFTNE
>Mature_281_residues
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLTAASIEAFRRNCDEAGFLPEHILPHDSYLINLGAPE
ADKIEKSRKAFVTEMQRAEALGLTMLNFHPGSHLNLTDEDACLKTIAESVNRSLDATAGVTAVIENTAGQGSNLGWRFEH
LARIIELVEDKSRVGVCLDTCHLFASGYDLRTPEAFDATLREFDRVVGLLYLKGMHLNDAKQKLGSKVDRHECLGKGMIG
IDAFAHIMRHPALEEIPLILETPNAEGWAEEIAMLYGFTNE

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=275, Percent_Identity=62.5454545454545, Blast_Score=354, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17531193, Length=277, Percent_Identity=47.6534296028881, Blast_Score=279, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6322735, Length=276, Percent_Identity=43.1159420289855, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_CHLTE (Q8KFL0)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661220.1
- ProteinModelPortal:   Q8KFL0
- SMR:   Q8KFL0
- GeneID:   1007977
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0316
- NMPDR:   fig|194439.1.peg.314
- TIGR:   CT0316
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   CTEP194439:CT_0316-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30932; Mature: 30932

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLTAASIEAFRRNCDEAG
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCC
FLPEHILPHDSYLINLGAPEADKIEKSRKAFVTEMQRAEALGLTMLNFHPGSHLNLTDED
CCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHH
ACLKTIAESVNRSLDATAGVTAVIENTAGQGSNLGWRFEHLARIIELVEDKSRVGVCLDT
HHHHHHHHHHCCCCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
CHLFASGYDLRTPEAFDATLREFDRVVGLLYLKGMHLNDAKQKLGSKVDRHECLGKGMIG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCHH
IDAFAHIMRHPALEEIPLILETPNAEGWAEEIAMLYGFTNE
HHHHHHHHHCCCHHHCCEEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRVGAHVSIAGGVENAPLNAQKIGAKAFAMFTRNQRQWHSAPLTAASIEAFRRNCDEAG
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCC
FLPEHILPHDSYLINLGAPEADKIEKSRKAFVTEMQRAEALGLTMLNFHPGSHLNLTDED
CCHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHH
ACLKTIAESVNRSLDATAGVTAVIENTAGQGSNLGWRFEHLARIIELVEDKSRVGVCLDT
HHHHHHHHHHCCCCCCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
CHLFASGYDLRTPEAFDATLREFDRVVGLLYLKGMHLNDAKQKLGSKVDRHECLGKGMIG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCHH
IDAFAHIMRHPALEEIPLILETPNAEGWAEEIAMLYGFTNE
HHHHHHHHHCCCHHHCCEEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901