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The map label for this gene is ribF [H]

Identifier: 21673083

GI number: 21673083

Start: 256006

End: 256971

Strand: Direct

Name: ribF [H]

Synonym: CT0244

Alternate gene names: 21673083

Gene position: 256006-256971 (Clockwise)

Preceding gene: 21673082

Following gene: 21673084

Centisome position: 11.88

GC content: 57.35

Gene sequence:

>966_bases
ATGCGCGTCGTAGTTTTGCAGGGCGATACGGTCCTTGATTCCGTAAACGGTTTGCCGGTTCAGCTTTCACCGGAGCCATC
GGCGGTGACCATCGGTTCGTTCGACGGACTGCATGTCGGTCACCGGAAGATCGTCGGTTCCATGATCGGACACGCCAGGG
AGCTGGGTCTCAGAAGCGTCGTGGTGACCTTCGAGCCCCATCCGAGAATCGTGCTCGAAGGTGGCGATGGCTGTCCGGTG
CGCTTGCTGACAACCTTCGACGAGAAGATCAGTCAGTTTAGCTCGATGCCGATTGACCTACTCTTCGTGGTGCGCTTTGA
CCGGCAGTTCGCCTCGAAAAGCTCCGAAGCGTTTATCCGCGAAGTGCTGGTGAAGATGCTCGGCGCGCGCCATGTCACCG
TCGGCTATGACCACGGCTTCGGCAAACGGCGGAGTGGAAGCGAAGAGACGCTGCACATGCTCGGCGCGGAGTGCGGCTTC
GGCGTCGATGTGGTCGGCGAAGTGATCGTTGCCGGTTCGCCGGTATCGAGCACTCGGATCAGAGGATTGCTTGAAGCGGC
AAGGATACGGGATGCCAACGAATGTCTCGGGGCACCGTACGCGATTAGCGGCACGGTGGTCGAAGGCGACAAACTTGGTC
GCACCATCGGGTTCCCGACGGTTAATCTCGCCCTTCCCGACCGTTGCAAAATGGTTCCGGCCCATGGAGTCTACGCAGCC
AGTATCGAGATTGACGGCAGGGAGTACTCTGCCATGATGAACATTGGTCGTCGTCCGACTGTATCGGAAGATGGCGAGGT
GAGGGTTGAGGCTCACATCATCGGGTTTTCAGGAGATCTGTATGGACGGTTTCTGATTGTGAGGATGCTCGATTTTATCA
GGGAAGAACGGCGTTTCGCATCAATCGACGAACTCCGGACGCAGCTCGAACTCGATAAAAAAGAGGCGGGATTCTGCAAG
AAATAA

Upstream 100 bases:

>100_bases
ATTGGTCAGTACCAGCTTTCGGATGCGATGAGCGTCGATGCTGTTGTTGACGAAATAACCCGTGCCGCTTCGGTCATCGA
AGAGTAAAACCGCAAGGAGT

Downstream 100 bases:

>100_bases
TGTTATATTAAAGGCTATTTTTTATTTCGTTATTTACTAACATATCATAGAGCAAACATCATGGGTCTGACAAAAGAACA
CAAAACCGAGATTATTACGA

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRVVVLQGDTVLDSVNGLPVQLSPEPSAVTIGSFDGLHVGHRKIVGSMIGHARELGLRSVVVTFEPHPRIVLEGGDGCPV
RLLTTFDEKISQFSSMPIDLLFVVRFDRQFASKSSEAFIREVLVKMLGARHVTVGYDHGFGKRRSGSEETLHMLGAECGF
GVDVVGEVIVAGSPVSSTRIRGLLEAARIRDANECLGAPYAISGTVVEGDKLGRTIGFPTVNLALPDRCKMVPAHGVYAA
SIEIDGREYSAMMNIGRRPTVSEDGEVRVEAHIIGFSGDLYGRFLIVRMLDFIREERRFASIDELRTQLELDKKEAGFCK
K

Sequences:

>Translated_321_residues
MRVVVLQGDTVLDSVNGLPVQLSPEPSAVTIGSFDGLHVGHRKIVGSMIGHARELGLRSVVVTFEPHPRIVLEGGDGCPV
RLLTTFDEKISQFSSMPIDLLFVVRFDRQFASKSSEAFIREVLVKMLGARHVTVGYDHGFGKRRSGSEETLHMLGAECGF
GVDVVGEVIVAGSPVSSTRIRGLLEAARIRDANECLGAPYAISGTVVEGDKLGRTIGFPTVNLALPDRCKMVPAHGVYAA
SIEIDGREYSAMMNIGRRPTVSEDGEVRVEAHIIGFSGDLYGRFLIVRMLDFIREERRFASIDELRTQLELDKKEAGFCK
K
>Mature_321_residues
MRVVVLQGDTVLDSVNGLPVQLSPEPSAVTIGSFDGLHVGHRKIVGSMIGHARELGLRSVVVTFEPHPRIVLEGGDGCPV
RLLTTFDEKISQFSSMPIDLLFVVRFDRQFASKSSEAFIREVLVKMLGARHVTVGYDHGFGKRRSGSEETLHMLGAECGF
GVDVVGEVIVAGSPVSSTRIRGLLEAARIRDANECLGAPYAISGTVVEGDKLGRTIGFPTVNLALPDRCKMVPAHGVYAA
SIEIDGREYSAMMNIGRRPTVSEDGEVRVEAHIIGFSGDLYGRFLIVRMLDFIREERRFASIDELRTQLELDKKEAGFCK
K

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=297, Percent_Identity=39.0572390572391, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17541882, Length=126, Percent_Identity=35.7142857142857, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 35070; Mature: 35070

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVVLQGDTVLDSVNGLPVQLSPEPSAVTIGSFDGLHVGHRKIVGSMIGHARELGLRSV
CEEEEEECCHHHHCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEE
VVTFEPHPRIVLEGGDGCPVRLLTTFDEKISQFSSMPIDLLFVVRFDRQFASKSSEAFIR
EEEECCCCEEEEECCCCCCEEEEEHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHH
EVLVKMLGARHVTVGYDHGFGKRRSGSEETLHMLGAECGFGVDVVGEVIVAGSPVSSTRI
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHEEECCCCCCHHHH
RGLLEAARIRDANECLGAPYAISGTVVEGDKLGRTIGFPTVNLALPDRCKMVPAHGVYAA
HHHHHHHHCCCCHHHCCCCEEEECEEEECHHCCCEECCCEEEEECCCCCCEEECCCEEEE
SIEIDGREYSAMMNIGRRPTVSEDGEVRVEAHIIGFSGDLYGRFLIVRMLDFIREERRFA
EEEECCHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
SIDELRTQLELDKKEAGFCKK
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRVVVLQGDTVLDSVNGLPVQLSPEPSAVTIGSFDGLHVGHRKIVGSMIGHARELGLRSV
CEEEEEECCHHHHCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEE
VVTFEPHPRIVLEGGDGCPVRLLTTFDEKISQFSSMPIDLLFVVRFDRQFASKSSEAFIR
EEEECCCCEEEEECCCCCCEEEEEHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHH
EVLVKMLGARHVTVGYDHGFGKRRSGSEETLHMLGAECGFGVDVVGEVIVAGSPVSSTRI
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHEEECCCCCCHHHH
RGLLEAARIRDANECLGAPYAISGTVVEGDKLGRTIGFPTVNLALPDRCKMVPAHGVYAA
HHHHHHHHCCCCHHHCCCCEEEECEEEECHHCCCEECCCEEEEECCCCCCEEECCCEEEE
SIEIDGREYSAMMNIGRRPTVSEDGEVRVEAHIIGFSGDLYGRFLIVRMLDFIREERRFA
EEEECCHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
SIDELRTQLELDKKEAGFCKK
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9454067; 9108293; 9384377 [H]