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The map label for this gene is truB

Identifier: 21673082

GI number: 21673082

Start: 255270

End: 255992

Strand: Direct

Name: truB

Synonym: CT0243

Alternate gene names: 21673082

Gene position: 255270-255992 (Clockwise)

Preceding gene: 21673081

Following gene: 21673083

Centisome position: 11.85

GC content: 56.85

Gene sequence:

>723_bases
ATGATCGATCAAGGCCGCATATCCGTTCTGAGCGAAGAGGGCGACTATCTGCTGGTGGACAAGCCGCTCGACTGGACATC
GTTCGATGTGGTGGCCAAGATTCGCGGCGCGTACAAGCGCAATGGCGCAAAGCGGAAGGTTGGGCACTGCGGCACGCTCG
ATCCAAAAGCCACCGGTTTGCTGATCCTTGCCACGGGGCGCAAGACCAAGACCATCTCGTCGCTTGAACTGCTCGACAAA
GCCTACGAAGGCACGATCAGGCTCGGAGCCAAAACGGTGAGTCACGATACGGAGAGCGAGGAGTATGATCTTCGCGACGT
GCCTAGCCTCGACGAGAGGGCGATCCGCGAAGCCGCCACGTCGATGATCGGCGAGCGGATGCAGCAGCCTCCAATGCACT
CGGCAGTCTGGCACAACGGCAAGCGCCTCTACGAACTGGCGCGGCAGGGTCACGAAGTCAAGGAGCGCAAGGCTCGCCAG
ATCGAGATTCACCAGTTCGAGATTACCGGTATTGAGTTGCCGTATGTCCATTTTTACATCAGGGTCTCCAAGGGTGCCTA
TATCCGGGTGATTGCCCATGAACTTGGGGAACTGCTTGGTGTTGGCGGTTATCTCAAATCATTGAAACGGGTTGCGATTG
GTCAGTACCAGCTTTCGGATGCGATGAGCGTCGATGCTGTTGTTGACGAAATAACCCGTGCCGCTTCGGTCATCGAAGAG
TAA

Upstream 100 bases:

>100_bases
TTCGAGCAGGCCAACCGGATCGAGCAACTGCTCAAATCGGTCAAGCCTGCCCGGGATGAAGAGCAGCATTAACTCCGCAT
TGTTTGGAATCGGGATCACG

Downstream 100 bases:

>100_bases
AACCGCAAGGAGTATGCGCGTCGTAGTTTTGCAGGGCGATACGGTCCTTGATTCCGTAAACGGTTTGCCGGTTCAGCTTT
CACCGGAGCCATCGGCGGTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MIDQGRISVLSEEGDYLLVDKPLDWTSFDVVAKIRGAYKRNGAKRKVGHCGTLDPKATGLLILATGRKTKTISSLELLDK
AYEGTIRLGAKTVSHDTESEEYDLRDVPSLDERAIREAATSMIGERMQQPPMHSAVWHNGKRLYELARQGHEVKERKARQ
IEIHQFEITGIELPYVHFYIRVSKGAYIRVIAHELGELLGVGGYLKSLKRVAIGQYQLSDAMSVDAVVDEITRAASVIEE

Sequences:

>Translated_240_residues
MIDQGRISVLSEEGDYLLVDKPLDWTSFDVVAKIRGAYKRNGAKRKVGHCGTLDPKATGLLILATGRKTKTISSLELLDK
AYEGTIRLGAKTVSHDTESEEYDLRDVPSLDERAIREAATSMIGERMQQPPMHSAVWHNGKRLYELARQGHEVKERKARQ
IEIHQFEITGIELPYVHFYIRVSKGAYIRVIAHELGELLGVGGYLKSLKRVAIGQYQLSDAMSVDAVVDEITRAASVIEE
>Mature_240_residues
MIDQGRISVLSEEGDYLLVDKPLDWTSFDVVAKIRGAYKRNGAKRKVGHCGTLDPKATGLLILATGRKTKTISSLELLDK
AYEGTIRLGAKTVSHDTESEEYDLRDVPSLDERAIREAATSMIGERMQQPPMHSAVWHNGKRLYELARQGHEVKERKARQ
IEIHQFEITGIELPYVHFYIRVSKGAYIRVIAHELGELLGVGGYLKSLKRVAIGQYQLSDAMSVDAVVDEITRAASVIEE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=211, Percent_Identity=26.5402843601896, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI2367200, Length=218, Percent_Identity=34.8623853211009, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=31.2820512820513, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323204, Length=204, Percent_Identity=31.8627450980392, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=142, Percent_Identity=32.3943661971831, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281364189, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364187, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364185, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364183, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62471759, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17975520, Length=208, Percent_Identity=29.3269230769231, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CHLTE (Q8KFS9)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661147.1
- ProteinModelPortal:   Q8KFS9
- SMR:   Q8KFS9
- GeneID:   1007733
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0243
- NMPDR:   fig|194439.1.peg.241
- TIGR:   CT0243
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   CLSK2516695
- BioCyc:   CTEP194439:CT_0243-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 26847; Mature: 26847

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQGRISVLSEEGDYLLVDKPLDWTSFDVVAKIRGAYKRNGAKRKVGHCGTLDPKATGL
CCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
LILATGRKTKTISSLELLDKAYEGTIRLGAKTVSHDTESEEYDLRDVPSLDERAIREAAT
EEEECCCCCHHHHHHHHHHHHHCCEEEECCEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
SMIGERMQQPPMHSAVWHNGKRLYELARQGHEVKERKARQIEIHQFEITGIELPYVHFYI
HHHHHHHHCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHEEEEEEEEEECEEECEEEEEE
RVSKGAYIRVIAHELGELLGVGGYLKSLKRVAIGQYQLSDAMSVDAVVDEITRAASVIEE
EECCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDQGRISVLSEEGDYLLVDKPLDWTSFDVVAKIRGAYKRNGAKRKVGHCGTLDPKATGL
CCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
LILATGRKTKTISSLELLDKAYEGTIRLGAKTVSHDTESEEYDLRDVPSLDERAIREAAT
EEEECCCCCHHHHHHHHHHHHHCCEEEECCEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
SMIGERMQQPPMHSAVWHNGKRLYELARQGHEVKERKARQIEIHQFEITGIELPYVHFYI
HHHHHHHHCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHEEEEEEEEEECEEECEEEEEE
RVSKGAYIRVIAHELGELLGVGGYLKSLKRVAIGQYQLSDAMSVDAVVDEITRAASVIEE
EECCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901