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The map label for this gene is vipA [H]

Identifier: 21673067

GI number: 21673067

Start: 241914

End: 243212

Strand: Reverse

Name: vipA [H]

Synonym: CT0227

Alternate gene names: 21673067

Gene position: 243212-241914 (Counterclockwise)

Preceding gene: 21673069

Following gene: 21673066

Centisome position: 11.29

GC content: 62.36

Gene sequence:

>1299_bases
ATGGACAAAACAAGGATCGCATTGATCGGCCTGGGTTACGTCGGCCTGCCTCTCGCCGTCGAGTTCGCGAAAAAATTTCC
GGTGGCCGGCTTCGACATCAAGCAGGAGCGGATCGAAGAGCTCAACAACGGGTGCGACTCGACGCTCGAAGTCGATGCGG
AGGCGCTCGGCGCGGTGCTCACAGCGAGCAACCCGCTGACCGCCGATGGCGACAAGGGCCTCTTCGTCACCTCCTCGATC
GCGGAGATCGCCAAAGCGAACATCTACATCGTCACCGTGCCGACACCGACCGACAAGCACAACCGCCCCGTGCTCACCCC
GCTCATCCGGGCGAGCGAAACGGTCGGACAGGTGCTCGAAAAGGGGGACATCGTCATCTACGAATCGACCGTCTATCCCG
GCGCTACCGAGGAGGATTGCGTGCCGGTGCTCGAACGGGTCTCGGGCCTCACGTTCAACCGGGACTTTTTCGTGGGCTAC
TCTCCCGAACGGATCAACCCCGGCGACAAGGAGCACACGGTCACGAAAATCAAAAAGGTGACCTCCGGCTCCACGCCCGA
AGCCGCCCGCAAAATCGACGAACTGTACGCTTCGGTCATCACGGCGGGCACGCACCTGGCCTCGTCGATCAGAGTCGCCG
AAGCGGCCAAGGTGATCGAGAACTCCCAGCGCGACATCAACATCGCCTTCGTCAACGAGCTGGCGATGATCTTCAATCGC
ATGGGCATCGACACGCTCGACGTGCTCCGGGCCGCCGGGACGAAGTGGAACTTCCTGCCCTTCCGGCCCGGTCTGGTCGG
CGGCCACTGCATCGGCGTCGATCCCTACTACCTGGCGCAGAAGGCTCAGGAGTACGGCTACCACCCGGAGATCATTCTGG
CGGGCCGCCGCCTGAACGACAACATGGGGCGCTACGTCGCCTCGGAGGTAGTCAAGCTGATGATTTCGCGCGACCTGAAG
GTCAAAGGCTCAAAGGTGCTCATGCTCGGCATCACCTTCAAGGAGAACTGCCCCGACATCCGCAACACCCGCGCCGTGGA
CATTGTGGCCGAACTGCGGGAGTACGGCGCGCAGGTGCACATTCACGACCCCTGGGCTAACCCGGAGGAGGTGCGACATG
AATATGGACTCGACTGTATCGACGCGCCGGTCGAAGGCGGTTACGACGCCATCATTCTCGCCGTCGCGCACGCGGCGTTC
GCGGAAATGGCCATCGGCAAACTCCGCTCTGGCGATAACGCCGTGATCTACGACGTGAAGGGCGTACTGCCAACCGACGC
CATCGACGGCAGACTCTGA

Upstream 100 bases:

>100_bases
ACCGAACGCGAACAGCCTCGCCGCCTGTCCGAAGAGCAAATCCCCGGAAGCGCCATAACGAAAATGCCTGAGCGAAACTC
TCAATGATCAACCAGTAACA

Downstream 100 bases:

>100_bases
GGCATGAAGCTTCTCTTCGTCAACTCCGCGCGGGAGTGGGGCGGCACAGAAAAATGGACGCGGATGGCCGCCCGCAAGCT
TTCGGAGCAGCATGAGGTCT

Product: UDP-glucose/GDP-mannose dehydrogenase family protein

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MDKTRIALIGLGYVGLPLAVEFAKKFPVAGFDIKQERIEELNNGCDSTLEVDAEALGAVLTASNPLTADGDKGLFVTSSI
AEIAKANIYIVTVPTPTDKHNRPVLTPLIRASETVGQVLEKGDIVIYESTVYPGATEEDCVPVLERVSGLTFNRDFFVGY
SPERINPGDKEHTVTKIKKVTSGSTPEAARKIDELYASVITAGTHLASSIRVAEAAKVIENSQRDINIAFVNELAMIFNR
MGIDTLDVLRAAGTKWNFLPFRPGLVGGHCIGVDPYYLAQKAQEYGYHPEIILAGRRLNDNMGRYVASEVVKLMISRDLK
VKGSKVLMLGITFKENCPDIRNTRAVDIVAELREYGAQVHIHDPWANPEEVRHEYGLDCIDAPVEGGYDAIILAVAHAAF
AEMAIGKLRSGDNAVIYDVKGVLPTDAIDGRL

Sequences:

>Translated_432_residues
MDKTRIALIGLGYVGLPLAVEFAKKFPVAGFDIKQERIEELNNGCDSTLEVDAEALGAVLTASNPLTADGDKGLFVTSSI
AEIAKANIYIVTVPTPTDKHNRPVLTPLIRASETVGQVLEKGDIVIYESTVYPGATEEDCVPVLERVSGLTFNRDFFVGY
SPERINPGDKEHTVTKIKKVTSGSTPEAARKIDELYASVITAGTHLASSIRVAEAAKVIENSQRDINIAFVNELAMIFNR
MGIDTLDVLRAAGTKWNFLPFRPGLVGGHCIGVDPYYLAQKAQEYGYHPEIILAGRRLNDNMGRYVASEVVKLMISRDLK
VKGSKVLMLGITFKENCPDIRNTRAVDIVAELREYGAQVHIHDPWANPEEVRHEYGLDCIDAPVEGGYDAIILAVAHAAF
AEMAIGKLRSGDNAVIYDVKGVLPTDAIDGRL
>Mature_432_residues
MDKTRIALIGLGYVGLPLAVEFAKKFPVAGFDIKQERIEELNNGCDSTLEVDAEALGAVLTASNPLTADGDKGLFVTSSI
AEIAKANIYIVTVPTPTDKHNRPVLTPLIRASETVGQVLEKGDIVIYESTVYPGATEEDCVPVLERVSGLTFNRDFFVGY
SPERINPGDKEHTVTKIKKVTSGSTPEAARKIDELYASVITAGTHLASSIRVAEAAKVIENSQRDINIAFVNELAMIFNR
MGIDTLDVLRAAGTKWNFLPFRPGLVGGHCIGVDPYYLAQKAQEYGYHPEIILAGRRLNDNMGRYVASEVVKLMISRDLK
VKGSKVLMLGITFKENCPDIRNTRAVDIVAELREYGAQVHIHDPWANPEEVRHEYGLDCIDAPVEGGYDAIILAVAHAAF
AEMAIGKLRSGDNAVIYDVKGVLPTDAIDGRL

Specific function: Catalyzes The Oxidation Of Udp-Mannac To Udp-N- Acetylmannosaminuronic Acid (Udp-Mannaca). [C]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994968, Length=444, Percent_Identity=31.3063063063063, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17560350, Length=385, Percent_Identity=24.4155844155844, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17136908, Length=388, Percent_Identity=24.4845360824742, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 47052; Mature: 47052

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTRIALIGLGYVGLPLAVEFAKKFPVAGFDIKQERIEELNNGCDSTLEVDAEALGAVL
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECHHHHCEEE
TASNPLTADGDKGLFVTSSIAEIAKANIYIVTVPTPTDKHNRPVLTPLIRASETVGQVLE
ECCCCCCCCCCCCEEEEHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
KGDIVIYESTVYPGATEEDCVPVLERVSGLTFNRDFFVGYSPERINPGDKEHTVTKIKKV
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCEECCEEEEECCCCCCCCCCCHHHHHHHHHH
TSGSTPEAARKIDELYASVITAGTHLASSIRVAEAAKVIENSQRDINIAFVNELAMIFNR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
MGIDTLDVLRAAGTKWNFLPFRPGLVGGHCIGVDPYYLAQKAQEYGYHPEIILAGRRLND
CCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCCC
NMGRYVASEVVKLMISRDLKVKGSKVLMLGITFKENCPDIRNTRAVDIVAELREYGAQVH
HHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEE
IHDPWANPEEVRHEYGLDCIDAPVEGGYDAIILAVAHAAFAEMAIGKLRSGDNAVIYDVK
EECCCCCHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GVLPTDAIDGRL
CCCCCCCCCCCC
>Mature Secondary Structure
MDKTRIALIGLGYVGLPLAVEFAKKFPVAGFDIKQERIEELNNGCDSTLEVDAEALGAVL
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEECHHHHCEEE
TASNPLTADGDKGLFVTSSIAEIAKANIYIVTVPTPTDKHNRPVLTPLIRASETVGQVLE
ECCCCCCCCCCCCEEEEHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
KGDIVIYESTVYPGATEEDCVPVLERVSGLTFNRDFFVGYSPERINPGDKEHTVTKIKKV
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCEECCEEEEECCCCCCCCCCCHHHHHHHHHH
TSGSTPEAARKIDELYASVITAGTHLASSIRVAEAAKVIENSQRDINIAFVNELAMIFNR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
MGIDTLDVLRAAGTKWNFLPFRPGLVGGHCIGVDPYYLAQKAQEYGYHPEIILAGRRLND
CCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEEECCCCCC
NMGRYVASEVVKLMISRDLKVKGSKVLMLGITFKENCPDIRNTRAVDIVAELREYGAQVH
HHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEE
IHDPWANPEEVRHEYGLDCIDAPVEGGYDAIILAVAHAAFAEMAIGKLRSGDNAVIYDVK
EECCCCCHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GVLPTDAIDGRL
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]