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The map label for this gene is capI [H]

Identifier: 21673069

GI number: 21673069

Start: 244017

End: 245069

Strand: Reverse

Name: capI [H]

Synonym: CT0230

Alternate gene names: 21673069

Gene position: 245069-244017 (Counterclockwise)

Preceding gene: 21673070

Following gene: 21673067

Centisome position: 11.37

GC content: 59.83

Gene sequence:

>1053_bases
ATGAAAATCCTCGTTACCGGCGCTGCCGGATTCATCGGCTTCCATCTTTGCGAACGACTCGCGTCGAGGGGCGACGACGT
GGTCGGCATCGACAACATCAACGACTACTACGACCAGCGGGTCAAGTACGGGCGTCTGGCGTACTCGGGCATCGCTGAAT
CCGCCATCGAATACGGCAAAACCGTGCAGTCGTCGAAATATCCGAACTACCGTTTCGTCAAGCTCAATCTCGAAGACAAG
GAGGGCATCGACAACCTCTTCAAGGCGGAGAAGTTCGACGCGCTCTGCAACCTGGCCGCCCAGGCGGGCGTGCGCTACTC
GCTCACCAATCCCGCCTCCTACGTCAGCTCGAACATCGTCGGCTTCGTGAACCTCTTGGAAGCGGCCCGCCACAACAGCC
TCGGCAACTTCTGCTATGCGTCAAGCTCATCGGTTTACGGCCTGAACGAACGCCAGCCCTTTTCGGTGCACGACAACGTC
GATCACCCGGTCAGCCTCTACGCGGCCAGCAAAAAGTCCAACGAGCTGATGGCCCACACCTACAGCCACCTGTTCGGCAT
TCCAACCACCGGCCTGCGCTTCTTCACGGTCTACGGCCCGTGGGGACGGCCCGACATGGCGCTCTTCCTCTTCACCAAAG
CCGCGCTCGAAGGCCGCCCGATCGATGTCTTCAACTATGGCAACATGCAGCGCGACTTCACCTACATCGACGACATCGTC
GAGGGCGTCGTGCGCGTGCTCGACCATCCGGCCCAGCCGAACCCCGACTGGAGCGGCGCGGCCCCCGATCCCGGCACCTC
GTCCGCCCCGTACCGCGTTTACAACATCGGCAACAACAAGACGGTCAAGCTGATGGATTACATCGAAGCACTCGAAAACG
CACTCGGTGTCACCATCGAAAAAAATCTGTTACCGATCCAGCCGGGCGACGTGCCTTCGACCTGGGCCAATGTCAGCGAC
CTGGTCAAAGACTTTGACTACAAGCCCGAAACCACGGTGCAGGAGGGCGTCAACCGTTTCATCGCCTGGTACCGCGAGTT
CTTCAAGGTCTGA

Upstream 100 bases:

>100_bases
AAGTTCACAAACACATTCCCGGCGCAGTCGAACCGCCAGATTGTGGCGTACCTGCTCCCGAAAACAGCCCCACAGATGGG
CTTCAAATCATACACACTGC

Downstream 100 bases:

>100_bases
GCGTTTACGGGCGACCTGTCGCCGAACAGAAAAAGCCGTGGCTGGAGTTTCCAACTGCTCCGGCCACGGCTTTCGGCGAT
CAAGTTGCTTCGGCGGGCTT

Product: NAD-dependent epimerase/dehydratase family protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKILVTGAAGFIGFHLCERLASRGDDVVGIDNINDYYDQRVKYGRLAYSGIAESAIEYGKTVQSSKYPNYRFVKLNLEDK
EGIDNLFKAEKFDALCNLAAQAGVRYSLTNPASYVSSNIVGFVNLLEAARHNSLGNFCYASSSSVYGLNERQPFSVHDNV
DHPVSLYAASKKSNELMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDIV
EGVVRVLDHPAQPNPDWSGAAPDPGTSSAPYRVYNIGNNKTVKLMDYIEALENALGVTIEKNLLPIQPGDVPSTWANVSD
LVKDFDYKPETTVQEGVNRFIAWYREFFKV

Sequences:

>Translated_350_residues
MKILVTGAAGFIGFHLCERLASRGDDVVGIDNINDYYDQRVKYGRLAYSGIAESAIEYGKTVQSSKYPNYRFVKLNLEDK
EGIDNLFKAEKFDALCNLAAQAGVRYSLTNPASYVSSNIVGFVNLLEAARHNSLGNFCYASSSSVYGLNERQPFSVHDNV
DHPVSLYAASKKSNELMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDIV
EGVVRVLDHPAQPNPDWSGAAPDPGTSSAPYRVYNIGNNKTVKLMDYIEALENALGVTIEKNLLPIQPGDVPSTWANVSD
LVKDFDYKPETTVQEGVNRFIAWYREFFKV
>Mature_350_residues
MKILVTGAAGFIGFHLCERLASRGDDVVGIDNINDYYDQRVKYGRLAYSGIAESAIEYGKTVQSSKYPNYRFVKLNLEDK
EGIDNLFKAEKFDALCNLAAQAGVRYSLTNPASYVSSNIVGFVNLLEAARHNSLGNFCYASSSSVYGLNERQPFSVHDNV
DHPVSLYAASKKSNELMAHTYSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDIV
EGVVRVLDHPAQPNPDWSGAAPDPGTSSAPYRVYNIGNNKTVKLMDYIEALENALGVTIEKNLLPIQPGDVPSTWANVSD
LVKDFDYKPETTVQEGVNRFIAWYREFFKV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=354, Percent_Identity=24.2937853107345, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI42516563, Length=353, Percent_Identity=24.0793201133144, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI56237023, Length=369, Percent_Identity=23.0352303523035, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI56118217, Length=369, Percent_Identity=23.0352303523035, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI189083684, Length=369, Percent_Identity=23.0352303523035, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788353, Length=371, Percent_Identity=26.1455525606469, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994969, Length=365, Percent_Identity=26.027397260274, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786974, Length=362, Percent_Identity=22.9281767955801, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=362, Percent_Identity=24.3093922651934, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=249, Percent_Identity=26.1044176706827, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=366, Percent_Identity=24.8633879781421, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=351, Percent_Identity=23.0769230769231, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=353, Percent_Identity=20.3966005665722, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=371, Percent_Identity=23.4501347708895, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=25.0696378830084, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39061; Mature: 39061

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGAAGFIGFHLCERLASRGDDVVGIDNINDYYDQRVKYGRLAYSGIAESAIEYGK
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TVQSSKYPNYRFVKLNLEDKEGIDNLFKAEKFDALCNLAAQAGVRYSLTNPASYVSSNIV
HHHCCCCCCEEEEEEECCCHHCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHH
GFVNLLEAARHNSLGNFCYASSSSVYGLNERQPFSVHDNVDHPVSLYAASKKSNELMAHT
HHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
YSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDIV
HHHHHCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHH
EGVVRVLDHPAQPNPDWSGAAPDPGTSSAPYRVYNIGNNKTVKLMDYIEALENALGVTIE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCEEE
KNLLPIQPGDVPSTWANVSDLVKDFDYKPETTVQEGVNRFIAWYREFFKV
CCEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVTGAAGFIGFHLCERLASRGDDVVGIDNINDYYDQRVKYGRLAYSGIAESAIEYGK
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
TVQSSKYPNYRFVKLNLEDKEGIDNLFKAEKFDALCNLAAQAGVRYSLTNPASYVSSNIV
HHHCCCCCCEEEEEEECCCHHCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHH
GFVNLLEAARHNSLGNFCYASSSSVYGLNERQPFSVHDNVDHPVSLYAASKKSNELMAHT
HHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
YSHLFGIPTTGLRFFTVYGPWGRPDMALFLFTKAALEGRPIDVFNYGNMQRDFTYIDDIV
HHHHHCCCCCCEEEEEEECCCCCCCCEEEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHH
EGVVRVLDHPAQPNPDWSGAAPDPGTSSAPYRVYNIGNNKTVKLMDYIEALENALGVTIE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCEEE
KNLLPIQPGDVPSTWANVSDLVKDFDYKPETTVQEGVNRFIAWYREFFKV
CCEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]