Click here to switch to the map view.

The map label for this gene is glnB [H]

Identifier: 21672975

GI number: 21672975

Start: 129582

End: 129923

Strand: Direct

Name: glnB [H]

Synonym: CT0134

Alternate gene names: 21672975

Gene position: 129582-129923 (Clockwise)

Preceding gene: 21672974

Following gene: 21672976

Centisome position: 6.01

GC content: 52.34

Gene sequence:

>342_bases
ATGAAACTGATAACCGCCATCATACAGCCCGACAGGCTCGACCATGTTCGCGAAGCGCTGATTCAGGCTGATATTACAAG
GATTACCGTCAGCCGGGTCTCTGGTCACGGACGCCAGGAGGATATCGAATACTACCGCGGCCAGAAAATCGCGCCGAACC
TGCTCCCGAAAGTTCGTCTCGATATCGCCGTTAATGACCAGTTTGTCAATGTTACCGTTGATACCATCGTTGCAGCCGCA
AGGCACGAAAGTGGTGAGATCGGCGATGGCAAGATATTTATCACTCCTTTGGAGGAGTGCGTGAGGATCAGAACCAACGA
ACGAGGAGGTAGCGCTATCTGA

Upstream 100 bases:

>100_bases
CGGCCTGCGTGCCAAAGAGAATGATGAAATGTCCGGTCTCGATCACAGTATGCACAGTGAACAGGGATATGGCCTTATCA
ATCCTAACTAATTACCAAAA

Downstream 100 bases:

>100_bases
TTGATACCGCTTTTGTTATCTGTTCGCTAAGCGAAAAGAAAATGCCGCAAAGGAGAAATCCCTGCGGCGTTTTCTTTTCA
TACCGATTCTTTATTTCATC

Product: P-II family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MKLITAIIQPDRLDHVREALIQADITRITVSRVSGHGRQEDIEYYRGQKIAPNLLPKVRLDIAVNDQFVNVTVDTIVAAA
RHESGEIGDGKIFITPLEECVRIRTNERGGSAI

Sequences:

>Translated_113_residues
MKLITAIIQPDRLDHVREALIQADITRITVSRVSGHGRQEDIEYYRGQKIAPNLLPKVRLDIAVNDQFVNVTVDTIVAAA
RHESGEIGDGKIFITPLEECVRIRTNERGGSAI
>Mature_113_residues
MKLITAIIQPDRLDHVREALIQADITRITVSRVSGHGRQEDIEYYRGQKIAPNLLPKVRLDIAVNDQFVNVTVDTIVAAA
RHESGEIGDGKIFITPLEECVRIRTNERGGSAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=114, Percent_Identity=48.2456140350877, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1786655, Length=114, Percent_Identity=44.7368421052632, Blast_Score=96, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12588; Mature: 12588

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITAIIQPDRLDHVREALIQADITRITVSRVSGHGRQEDIEYYRGQKIAPNLLPKVRL
CCCEEEEECCCHHHHHHHHHHHHHHHHEEEEHHCCCCCCHHHHHHCCCCCCCCCCCEEEE
DIAVNDQFVNVTVDTIVAAARHESGEIGDGKIFITPLEECVRIRTNERGGSAI
EEEECCEEEEEEHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLITAIIQPDRLDHVREALIQADITRITVSRVSGHGRQEDIEYYRGQKIAPNLLPKVRL
CCCEEEEECCCHHHHHHHHHHHHHHHHEEEEHHCCCCCCHHHHHHCCCCCCCCCCCEEEE
DIAVNDQFVNVTVDTIVAAARHESGEIGDGKIFITPLEECVRIRTNERGGSAI
EEEECCEEEEEEHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1702507; 2878685 [H]