Click here to switch to the map view.

The map label for this gene is amt-3 [H]

Identifier: 21672974

GI number: 21672974

Start: 128241

End: 129572

Strand: Direct

Name: amt-3 [H]

Synonym: CT0133

Alternate gene names: 21672974

Gene position: 128241-129572 (Clockwise)

Preceding gene: 21672972

Following gene: 21672975

Centisome position: 5.95

GC content: 57.81

Gene sequence:

>1332_bases
ATGGCGAAAAAAATCAGTGCGGCGGTGATGCTGTTTTTTGCAGTGATGATGGCGCATGGCACCGTTTGGGCAGGCGAGCC
GGTGAGCATCGATACCGGCACGACATCCTGGATGCTGACCTCCACGGCGCTCGTGTTGCTCATGGTGCCAGGGTTGGCGA
TGTTCTATGGCGGCCTGGTCAGGACGAAGAATGTTATCGGCACGATGATGCACAGCTTTGGCGCTATGGTCATCATTGGC
GTCTTGTGGCCACTGGTCGGATACTCGCTCAGCTTTGGCCACGGTATTCTCGGCGGCCTGGTTGGATGGGATCCGAAATA
TTTCATGTTGCAGGGGATTGATGATACCATCATGGCGAGTCATATCCCTGAATATGTCTTTGCCATGTTCCAGGGCAAAT
TCGCCATCATCACTCCTGCGCTGATTGCTGGTGCTTTTGCCGAGCGGGTCAATTTCAAGGGGTATCTGTTGTTTATTGCC
TTGTGGAGCATTTTCGTGTACAGCCCGATCTGCCACTGGGTCTGGGCTGGCGACGGTTTTCTGTTCAATCTCGGCGCGAT
GGGCGCTATCGATTTTGCTGGTGGCACGGTCGTGCACATCTCTTCCGGCGTGACCGGCCTTGTCGCGGCGCTCTTCCTTG
GCTCGCGTCGCGGATATCCGAGCAACGTCACCTCGCCGAACAACCTGGTCATGACCCTCGTGGGTGCGGGCCTGCTCTGG
GTGGGATGGTTCGGTTTCAATGCTGGCAGCGCCATCGCCAGCGATCTTGCCACGGCTCGCGCACTGACCGTGACCCAGGT
TGCCGCGGCGTCGGGCGCATTCACCTGGCTCATCATCGAACTGGTGCATCACAATAAGGCAACCAGCCTTGGCGTGGCAT
CGGGTATTCTGGCTGGTCTGGTGGCGATCACCCCTGCCGCGGGCGTCGTGCAGCCATCGGGCGCGTTCGCGCTTGGCGCT
ATTGCCGCCATTGTCTGCTACCTCGGCCTCATGCTGAAGAGCAAGCTCGGCTACGATGACAGCCTCGATGCTTTCGGCGT
GCACGGTGTTGGCGGTATTGTCGGCGCTCTCTGCCTGATTTTCTTCATTCGCCCGTCGTGGATGGCCGACGCCGCTGTCA
AGGCTGGCGGAAGCTGGACGGTCTGGCAGCAGCTTGGCGTTCAGGCTACGGCTGTCGGGATTACGGTCGTCTATGCGGCA
GTTGTTTCACTGGTGATCCTGTTCATCGTCGAGAAAACCATCGGCCTGCGTGCCAAAGAGAATGATGAAATGTCCGGTCT
CGATCACAGTATGCACAGTGAACAGGGATATGGCCTTATCAATCCTAACTAA

Upstream 100 bases:

>100_bases
AGAAAATGGCTTAAAAATTTGCTTGTGCTTTAATATTTAATTATAATTCGCATTCTTTCATAAAAGATTTAGGTGTTTGA
AATTTTTAAACAAGAAGGTT

Downstream 100 bases:

>100_bases
TTACCAAAAATGAAACTGATAACCGCCATCATACAGCCCGACAGGCTCGACCATGTTCGCGAAGCGCTGATTCAGGCTGA
TATTACAAGGATTACCGTCA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAKKISAAVMLFFAVMMAHGTVWAGEPVSIDTGTTSWMLTSTALVLLMVPGLAMFYGGLVRTKNVIGTMMHSFGAMVIIG
VLWPLVGYSLSFGHGILGGLVGWDPKYFMLQGIDDTIMASHIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYLLFIA
LWSIFVYSPICHWVWAGDGFLFNLGAMGAIDFAGGTVVHISSGVTGLVAALFLGSRRGYPSNVTSPNNLVMTLVGAGLLW
VGWFGFNAGSAIASDLATARALTVTQVAAASGAFTWLIIELVHHNKATSLGVASGILAGLVAITPAAGVVQPSGAFALGA
IAAIVCYLGLMLKSKLGYDDSLDAFGVHGVGGIVGALCLIFFIRPSWMADAAVKAGGSWTVWQQLGVQATAVGITVVYAA
VVSLVILFIVEKTIGLRAKENDEMSGLDHSMHSEQGYGLINPN

Sequences:

>Translated_443_residues
MAKKISAAVMLFFAVMMAHGTVWAGEPVSIDTGTTSWMLTSTALVLLMVPGLAMFYGGLVRTKNVIGTMMHSFGAMVIIG
VLWPLVGYSLSFGHGILGGLVGWDPKYFMLQGIDDTIMASHIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYLLFIA
LWSIFVYSPICHWVWAGDGFLFNLGAMGAIDFAGGTVVHISSGVTGLVAALFLGSRRGYPSNVTSPNNLVMTLVGAGLLW
VGWFGFNAGSAIASDLATARALTVTQVAAASGAFTWLIIELVHHNKATSLGVASGILAGLVAITPAAGVVQPSGAFALGA
IAAIVCYLGLMLKSKLGYDDSLDAFGVHGVGGIVGALCLIFFIRPSWMADAAVKAGGSWTVWQQLGVQATAVGITVVYAA
VVSLVILFIVEKTIGLRAKENDEMSGLDHSMHSEQGYGLINPN
>Mature_442_residues
AKKISAAVMLFFAVMMAHGTVWAGEPVSIDTGTTSWMLTSTALVLLMVPGLAMFYGGLVRTKNVIGTMMHSFGAMVIIGV
LWPLVGYSLSFGHGILGGLVGWDPKYFMLQGIDDTIMASHIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYLLFIAL
WSIFVYSPICHWVWAGDGFLFNLGAMGAIDFAGGTVVHISSGVTGLVAALFLGSRRGYPSNVTSPNNLVMTLVGAGLLWV
GWFGFNAGSAIASDLATARALTVTQVAAASGAFTWLIIELVHHNKATSLGVASGILAGLVAITPAAGVVQPSGAFALGAI
AAIVCYLGLMLKSKLGYDDSLDAFGVHGVGGIVGALCLIFFIRPSWMADAAVKAGGSWTVWQQLGVQATAVGITVVYAAV
VSLVILFIVEKTIGLRAKENDEMSGLDHSMHSEQGYGLINPN

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=413, Percent_Identity=40.1937046004843, Blast_Score=273, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32564042, Length=419, Percent_Identity=27.9236276849642, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=434, Percent_Identity=27.1889400921659, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=28.0898876404494, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17550182, Length=443, Percent_Identity=28.6681715575621, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=426, Percent_Identity=33.0985915492958, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=33.0232558139535, Blast_Score=205, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321559, Length=426, Percent_Identity=29.3427230046948, Blast_Score=192, Evalue=8e-50,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=31.3253012048193, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46455; Mature: 46324

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKISAAVMLFFAVMMAHGTVWAGEPVSIDTGTTSWMLTSTALVLLMVPGLAMFYGGLV
CCCHHHHHHHHHHHHHHHCCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RTKNVIGTMMHSFGAMVIIGVLWPLVGYSLSFGHGILGGLVGWDPKYFMLQGIDDTIMAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCCCCEEEEECCCHHHHHH
HIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYLLFIALWSIFVYSPICHWVWAGDGF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFNLGAMGAIDFAGGTVVHISSGVTGLVAALFLGSRRGYPSNVTSPNNLVMTLVGAGLLW
EEECCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
VGWFGFNAGSAIASDLATARALTVTQVAAASGAFTWLIIELVHHNKATSLGVASGILAGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VAITPAAGVVQPSGAFALGAIAAIVCYLGLMLKSKLGYDDSLDAFGVHGVGGIVGALCLI
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FFIRPSWMADAAVKAGGSWTVWQQLGVQATAVGITVVYAAVVSLVILFIVEKTIGLRAKE
HHHCCCHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NDEMSGLDHSMHSEQGYGLINPN
CCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AKKISAAVMLFFAVMMAHGTVWAGEPVSIDTGTTSWMLTSTALVLLMVPGLAMFYGGLV
CCHHHHHHHHHHHHHHHCCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RTKNVIGTMMHSFGAMVIIGVLWPLVGYSLSFGHGILGGLVGWDPKYFMLQGIDDTIMAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHCCCCCCEEEEECCCHHHHHH
HIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYLLFIALWSIFVYSPICHWVWAGDGF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFNLGAMGAIDFAGGTVVHISSGVTGLVAALFLGSRRGYPSNVTSPNNLVMTLVGAGLLW
EEECCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
VGWFGFNAGSAIASDLATARALTVTQVAAASGAFTWLIIELVHHNKATSLGVASGILAGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VAITPAAGVVQPSGAFALGAIAAIVCYLGLMLKSKLGYDDSLDAFGVHGVGGIVGALCLI
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FFIRPSWMADAAVKAGGSWTVWQQLGVQATAVGITVVYAAVVSLVILFIVEKTIGLRAKE
HHHCCCHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NDEMSGLDHSMHSEQGYGLINPN
CCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]