Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 21672964

GI number: 21672964

Start: 116816

End: 117889

Strand: Reverse

Name: prfA

Synonym: CT0123

Alternate gene names: 21672964

Gene position: 117889-116816 (Counterclockwise)

Preceding gene: 21672965

Following gene: 21672963

Centisome position: 5.47

GC content: 62.1

Gene sequence:

>1074_bases
ATGTTTGACAAGCTTCAGTCCATCAAGGATAAATTCCAGACCATCGAGCAGCAGCTTTCCGATCCGGAGGTGGTCGCCGA
CCAGAATCGCTTCAGGAAGCTGAACAAGGAGTACAGCAGCCTGAAGGAGATCGTGCGCGCTTACGACGAGTGGAGCCGAA
CCAAAAAGCAGCTCGACGAGGCGCATTCGATGCAGAAAAACGAAAATGATCCGGAAATGCGCGCCCTCGTCGAAGAGGAG
GCCGGCGAACTCCAGGAGCGCCTCCCGAAGCTTGAGCAGCAGCTCAAAATCCTGCTGCTGCCAAAAGACGAGGCCGACAG
CCGCAACGCCATCATTGAAATCCGCGCGGGAACCGGTGGCGACGAAGCGGGGCTGTTCGCTGCCGACCTGATGCGCATGT
ACCAGCGCTATGCCGAACGGCAGGGGTGGAGCTGCCAGACGCTCGAAGTGAGCGAAGGCTCAGTGCCCGGCAGCCTCAAG
GAGGTTTCGCTTGAAGTGAGCGGCCACAACGTCTACGGTATCCTCAAGTTCGAGAGCGGTGTGCACCGCGTGCAGCGCGT
GCCCGAAACCGAAACGCAGGGGCGCATCCACACCTCGGCGGCCAGCGTCGCCGTACTCCCCGAAGCCGAAGAGGTGGACG
TGGAGATCCGCAAGGAAGATTTGCTGATAGACACCTTTCGCAGTGGCGGCAAAGGCGGCCAGAACGTCAACAAGGTGGAA
ACCGCCGTGCGAATCACCCACGTCCCGAGCGGCATCGTCGTCGCCTGCCAGGAGGAACGCTCGCAGCTCCAGAACCGCGA
GCGGGCCATGAAGATGCTGCGCTCGAAGCTCTACGACCTCCAGATCGCCGAGCAGCAAAAGAGCCGCGCCGACCTGCGCC
GCTCGATGGTAACCACCGGCGACCGGAGCGCCAAGATCAGGACCTACAACTTTCCCCAGTCGCGCGTGACCGACCACCGC
ATCGGCTTCACCAGCCACGCCCTGCCGCAAATCATGCAGGGCGAGCTCGACCCGCTCATCGAGGCGCTGCGAATGCACGA
CCAGGCCGAGCGCTTGCAGGCCGAAACCGCCTGA

Upstream 100 bases:

>100_bases
GCGTATCCAGCAGGTCGGCATGACTCTTCTGCTGATGCTCTTCGCCTACTTCATGATAAACGACCTCCTGAACCCCTGAT
TCCGGCAGCGGCGAGCAACC

Downstream 100 bases:

>100_bases
CCTCGTTGCCCGCGATGCACGCGCGGATGTGCGCAATTTTGCCGGGAACTCGCTATATTTTCAGAGATAACGCCGACCAC
CCGGCCATTTTTTTCAACCA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFDKLQSIKDKFQTIEQQLSDPEVVADQNRFRKLNKEYSSLKEIVRAYDEWSRTKKQLDEAHSMQKNENDPEMRALVEEE
AGELQERLPKLEQQLKILLLPKDEADSRNAIIEIRAGTGGDEAGLFAADLMRMYQRYAERQGWSCQTLEVSEGSVPGSLK
EVSLEVSGHNVYGILKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEEVDVEIRKEDLLIDTFRSGGKGGQNVNKVE
TAVRITHVPSGIVVACQEERSQLQNRERAMKMLRSKLYDLQIAEQQKSRADLRRSMVTTGDRSAKIRTYNFPQSRVTDHR
IGFTSHALPQIMQGELDPLIEALRMHDQAERLQAETA

Sequences:

>Translated_357_residues
MFDKLQSIKDKFQTIEQQLSDPEVVADQNRFRKLNKEYSSLKEIVRAYDEWSRTKKQLDEAHSMQKNENDPEMRALVEEE
AGELQERLPKLEQQLKILLLPKDEADSRNAIIEIRAGTGGDEAGLFAADLMRMYQRYAERQGWSCQTLEVSEGSVPGSLK
EVSLEVSGHNVYGILKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEEVDVEIRKEDLLIDTFRSGGKGGQNVNKVE
TAVRITHVPSGIVVACQEERSQLQNRERAMKMLRSKLYDLQIAEQQKSRADLRRSMVTTGDRSAKIRTYNFPQSRVTDHR
IGFTSHALPQIMQGELDPLIEALRMHDQAERLQAETA
>Mature_357_residues
MFDKLQSIKDKFQTIEQQLSDPEVVADQNRFRKLNKEYSSLKEIVRAYDEWSRTKKQLDEAHSMQKNENDPEMRALVEEE
AGELQERLPKLEQQLKILLLPKDEADSRNAIIEIRAGTGGDEAGLFAADLMRMYQRYAERQGWSCQTLEVSEGSVPGSLK
EVSLEVSGHNVYGILKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEEVDVEIRKEDLLIDTFRSGGKGGQNVNKVE
TAVRITHVPSGIVVACQEERSQLQNRERAMKMLRSKLYDLQIAEQQKSRADLRRSMVTTGDRSAKIRTYNFPQSRVTDHR
IGFTSHALPQIMQGELDPLIEALRMHDQAERLQAETA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=299, Percent_Identity=45.1505016722408, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=38.10888252149, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=42.8571428571429, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=49.1477272727273, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=36.1194029850746, Blast_Score=212, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=292, Percent_Identity=40.4109589041096, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=326, Percent_Identity=39.5705521472393, Blast_Score=231, Evalue=9e-62,
Organism=Drosophila melanogaster, GI19921226, Length=326, Percent_Identity=42.0245398773006, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CHLTE (Q8KG45)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661029.1
- ProteinModelPortal:   Q8KG45
- SMR:   Q8KG45
- GeneID:   1006803
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0123
- NMPDR:   fig|194439.1.peg.123
- TIGR:   CT0123
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- BioCyc:   CTEP194439:CT_0123-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40601; Mature: 40601

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKLQSIKDKFQTIEQQLSDPEVVADQNRFRKLNKEYSSLKEIVRAYDEWSRTKKQLDE
CCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHSMQKNENDPEMRALVEEEAGELQERLPKLEQQLKILLLPKDEADSRNAIIEIRAGTGG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
DEAGLFAADLMRMYQRYAERQGWSCQTLEVSEGSVPGSLKEVSLEVSGHNVYGILKFESG
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCEEEEEEEECHH
VHRVQRVPETETQGRIHTSAASVAVLPEAEEVDVEIRKEDLLIDTFRSGGKGGQNVNKVE
HHHHHHCCCCCCCCEEEECCCCEEECCCCHHCCCEEHHHHHHHHHHHCCCCCCCCHHHHH
TAVRITHVPSGIVVACQEERSQLQNRERAMKMLRSKLYDLQIAEQQKSRADLRRSMVTTG
HHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRSAKIRTYNFPQSRVTDHRIGFTSHALPQIMQGELDPLIEALRMHDQAERLQAETA
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFDKLQSIKDKFQTIEQQLSDPEVVADQNRFRKLNKEYSSLKEIVRAYDEWSRTKKQLDE
CCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHSMQKNENDPEMRALVEEEAGELQERLPKLEQQLKILLLPKDEADSRNAIIEIRAGTGG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
DEAGLFAADLMRMYQRYAERQGWSCQTLEVSEGSVPGSLKEVSLEVSGHNVYGILKFESG
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCEEEEEEEECHH
VHRVQRVPETETQGRIHTSAASVAVLPEAEEVDVEIRKEDLLIDTFRSGGKGGQNVNKVE
HHHHHHCCCCCCCCEEEECCCCEEECCCCHHCCCEEHHHHHHHHHHHCCCCCCCCHHHHH
TAVRITHVPSGIVVACQEERSQLQNRERAMKMLRSKLYDLQIAEQQKSRADLRRSMVTTG
HHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRSAKIRTYNFPQSRVTDHRIGFTSHALPQIMQGELDPLIEALRMHDQAERLQAETA
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901