Click here to switch to the map view.

The map label for this gene is aprF [H]

Identifier: 21672948

GI number: 21672948

Start: 100511

End: 101797

Strand: Reverse

Name: aprF [H]

Synonym: CT0107

Alternate gene names: 21672948

Gene position: 101797-100511 (Counterclockwise)

Preceding gene: 21672951

Following gene: 21672947

Centisome position: 4.72

GC content: 61.46

Gene sequence:

>1287_bases
ATGATCGCCGTTTCCTTTTCGGCGGGCGAGCCTTTGCTGGCGGCTGAAACCAGCGGCGCGCCGCTGACACTCGACGAGGC
GCTGCGCATGACGCGCGAGCACAACCCGAAAGCCCGGCAGGCCTTGGAGGAGTTGAACGCTGCCGACGCGAAAGTCACCG
AAAGCCGCAGCGCCTGGTTTCCGCAAATCTCGGGCAAGGCGGGTTACACCTACCTCGATCCGGTCTCCGAAATGACCTTC
GGCGGCCTCGCCATGAAGTTCATGCCGAACAACAATTACAATGCGCGATTCACCGCCGAGATGATGCTCTACGATTTCGG
GCGCACCGCAAGCACCGTCGATCTGGCCAAAGCGGCTCGCAACTCGGCAAGGCTCCGGCAGGACATGACCCTCCGCGACC
TTTCGCTGGCGACCGTGCAGGCGTTCTACTCGGTGCTCTTTTTGCAGGAGGCGGTCAGGGTTCAGCAGAAGGAGGTTGCC
GCGCTGCAAACGAACCTCGACCACATGCAGAAGCGCTATGACCAGGGTGCAGCCACCCGCTTCGACTTGCTGACGACCCA
GGTGCGGCTGGCGGGCGCGGCCAATCGCGAGATCGATTACCAGAACCAGCTCCGCAATCAGGAGATTACGCTGCGTCGCC
TCTGTGGTCTCGACGAAAAAGCGCCACTGAGCCTGAAGGGGTCGTTTGATATTACGGCTGCCGATATGGATGCCGACAAG
CTTGCCGCTTCGGCGCTCGACCACCGGCCGGAGGTGATGCTCGCCCGCGAGAATTTCAAGGCGGCGTCGTACAAAAAAAA
TCTCGCTACCCGTGAGTTTCTGCCGAAGATCGTAGGCAGCGCTTCGTGGGGAAGCACCAATGGCTATGTGCCCGACCTCG
ACAAGATGCGCACCAACGTCGCGGTGGGCGTCGAGCTTCAGGTGCCGATTTTTGATGGATTCAGGAAGAGCGCCGCGCTC
AGGGAGGCTACGGCGATGAAGCGCTCGGCGGAGCAGCAGCAGCTCGACGCCGAGCAGGTGAGTCAGGCCGAGGTGCGCCA
GTCGGTCAACGATCTGAAGAGCAGCGCCGAAAAAATCCAGACCACCCGCTTGCAGGTTTCGCAGGCTGATCTTGCGGCCA
AACACGCGCGAATCCGCTACGACAATGGGCTGGCCACCACGCTCGATCTGCTCGACGCCGAAGCGGCGCTGGCCGAAGCC
GAGCTGGCGAACTTGCAGGCCCGATATGAATATGTGATGAATGCCTATTCGGTCAGACGCGCCGCTGGCGATCTGATCGA
GCGATAA

Upstream 100 bases:

>100_bases
CGTGAAAAAGAAATTATGGCATGGAAATTGATGTTGATTTCCTGTCGATTTAACCATGTCGCATACTATGAAAAAAAGAA
ATACCATCGCCGCCCTGCTC

Downstream 100 bases:

>100_bases
ACGGAGTGATAATCATGACGACAAACCATGTAAAACCATCGAAAATTATGCTCTGCCCTGTCGATTTTTCGCCATCTTCC
GAAAGGGCGCTGCTTTATGC

Product: outer membrane protein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MIAVSFSAGEPLLAAETSGAPLTLDEALRMTREHNPKARQALEELNAADAKVTESRSAWFPQISGKAGYTYLDPVSEMTF
GGLAMKFMPNNNYNARFTAEMMLYDFGRTASTVDLAKAARNSARLRQDMTLRDLSLATVQAFYSVLFLQEAVRVQQKEVA
ALQTNLDHMQKRYDQGAATRFDLLTTQVRLAGAANREIDYQNQLRNQEITLRRLCGLDEKAPLSLKGSFDITAADMDADK
LAASALDHRPEVMLARENFKAASYKKNLATREFLPKIVGSASWGSTNGYVPDLDKMRTNVAVGVELQVPIFDGFRKSAAL
REATAMKRSAEQQQLDAEQVSQAEVRQSVNDLKSSAEKIQTTRLQVSQADLAAKHARIRYDNGLATTLDLLDAEAALAEA
ELANLQARYEYVMNAYSVRRAAGDLIER

Sequences:

>Translated_428_residues
MIAVSFSAGEPLLAAETSGAPLTLDEALRMTREHNPKARQALEELNAADAKVTESRSAWFPQISGKAGYTYLDPVSEMTF
GGLAMKFMPNNNYNARFTAEMMLYDFGRTASTVDLAKAARNSARLRQDMTLRDLSLATVQAFYSVLFLQEAVRVQQKEVA
ALQTNLDHMQKRYDQGAATRFDLLTTQVRLAGAANREIDYQNQLRNQEITLRRLCGLDEKAPLSLKGSFDITAADMDADK
LAASALDHRPEVMLARENFKAASYKKNLATREFLPKIVGSASWGSTNGYVPDLDKMRTNVAVGVELQVPIFDGFRKSAAL
REATAMKRSAEQQQLDAEQVSQAEVRQSVNDLKSSAEKIQTTRLQVSQADLAAKHARIRYDNGLATTLDLLDAEAALAEA
ELANLQARYEYVMNAYSVRRAAGDLIER
>Mature_428_residues
MIAVSFSAGEPLLAAETSGAPLTLDEALRMTREHNPKARQALEELNAADAKVTESRSAWFPQISGKAGYTYLDPVSEMTF
GGLAMKFMPNNNYNARFTAEMMLYDFGRTASTVDLAKAARNSARLRQDMTLRDLSLATVQAFYSVLFLQEAVRVQQKEVA
ALQTNLDHMQKRYDQGAATRFDLLTTQVRLAGAANREIDYQNQLRNQEITLRRLCGLDEKAPLSLKGSFDITAADMDADK
LAASALDHRPEVMLARENFKAASYKKNLATREFLPKIVGSASWGSTNGYVPDLDKMRTNVAVGVELQVPIFDGFRKSAAL
REATAMKRSAEQQQLDAEQVSQAEVRQSVNDLKSSAEKIQTTRLQVSQADLAAKHARIRYDNGLATTLDLLDAEAALAEA
ELANLQARYEYVMNAYSVRRAAGDLIER

Specific function: Involved in the secretion of alkaline protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=430, Percent_Identity=25.3488372093023, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 47365; Mature: 47365

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVSFSAGEPLLAAETSGAPLTLDEALRMTREHNPKARQALEELNAADAKVTESRSAWF
CEEEEECCCCCEEEECCCCCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCC
PQISGKAGYTYLDPVSEMTFGGLAMKFMPNNNYNARFTAEMMLYDFGRTASTVDLAKAAR
CCCCCCCCCEEECCHHHHHHCCEEEEEECCCCCCCEEEHEEHHHHCCCCCHHHHHHHHHH
NSARLRQDMTLRDLSLATVQAFYSVLFLQEAVRVQQKEVAALQTNLDHMQKRYDQGAATR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FDLLTTQVRLAGAANREIDYQNQLRNQEITLRRLCGLDEKAPLSLKGSFDITAADMDADK
HHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCEEECCCCEEEEECCCHHH
LAASALDHRPEVMLARENFKAASYKKNLATREFLPKIVGSASWGSTNGYVPDLDKMRTNV
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCE
AVGVELQVPIFDGFRKSAALREATAMKRSAEQQQLDAEQVSQAEVRQSVNDLKSSAEKIQ
EEEEEEEECHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TTRLQVSQADLAAKHARIRYDNGLATTLDLLDAEAALAEAELANLQARYEYVMNAYSVRR
HHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGDLIER
HHHHHHCC
>Mature Secondary Structure
MIAVSFSAGEPLLAAETSGAPLTLDEALRMTREHNPKARQALEELNAADAKVTESRSAWF
CEEEEECCCCCEEEECCCCCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCCCCC
PQISGKAGYTYLDPVSEMTFGGLAMKFMPNNNYNARFTAEMMLYDFGRTASTVDLAKAAR
CCCCCCCCCEEECCHHHHHHCCEEEEEECCCCCCCEEEHEEHHHHCCCCCHHHHHHHHHH
NSARLRQDMTLRDLSLATVQAFYSVLFLQEAVRVQQKEVAALQTNLDHMQKRYDQGAATR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FDLLTTQVRLAGAANREIDYQNQLRNQEITLRRLCGLDEKAPLSLKGSFDITAADMDADK
HHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCEEECCCCEEEEECCCHHH
LAASALDHRPEVMLARENFKAASYKKNLATREFLPKIVGSASWGSTNGYVPDLDKMRTNV
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCE
AVGVELQVPIFDGFRKSAALREATAMKRSAEQQQLDAEQVSQAEVRQSVNDLKSSAEKIQ
EEEEEEEECHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TTRLQVSQADLAAKHARIRYDNGLATTLDLLDAEAALAEAELANLQARYEYVMNAYSVRR
HHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGDLIER
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1427098; 10984043 [H]