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The map label for this gene is prfC [H]

Identifier: 57634623

GI number: 57634623

Start: 1070905

End: 1072107

Strand: Direct

Name: prfC [H]

Synonym: SAV1021

Alternate gene names: 57634623

Gene position: 1070905-1072107 (Clockwise)

Preceding gene: 57634622

Following gene: 15924012

Centisome position: 37.2

GC content: 33.08

Gene sequence:

>1203_bases
TTGAAGTTATTTAAAGTTTGTAAAATGCGTGGTATTCCAATCTTTACATTCATTAATAAATTAGACCGAGTAGGTAAAGA
ACCATTTGAATTATTAGATGAAATCGAAGAGACATTAAATATTGAAACATACCCTATGAATTGGCCAATTGGTATGGGAC
AAAGTTTCTTTGGCATCATTGATAGAAAGTCTAAAACAATTGAACCATTTAGAGATGAAGAAAATATATTACATTTGAAT
GATGATTTTGAGTTGGAAGAAGATCATGCAATTACAAATGATAGTGCTTTTGAACAAGCGATTGAAGAATTAATGTTGGT
TGAAGAAGCGGGTGAAGCCTTTGATAATGACGCGCTGTTGAGTGGAGACTTAACACCTGTATTTTTCGGTTCAGCTTTAG
CTAACTTTGGTGTACAAAATTTCTTAAATGCATATGTTGATTTTGCGCCAATGCCAAATGCGAGACAAACAAAAGAAGAC
GTTGAAGTAAGCCCGTTTGATGATTCATTTTCAGGATTTATCTTTAAAATTCAAGCCAACATGGACCCTAAACACCGTGA
TAGAATTGCCTTTATGCGTGTCGTTAGTGGTGCATTTGAACGTGGTATGGATGTTACTTTGCAACGTACTAATAAAAAGC
AAAAGATCACACGTTCAACGTCATTTATGGCAGACGATAAAGAAACTGTGAATCATGCTGTAGCAGGCGATATCATTGGA
CTATATGATACTGGTAATTATCAAATTGGAGATACTTTAGTTGGTGGAAAACAAACCTACAGTTTCCAAGATTTACCACA
ATTTACGCCAGAAATTTTTATGAAAGTTTCTGCTAAAAACGTCATGAAACAGAAGCATTTCCATAAAGGTATTGAACAAT
TAGTACAAGAAGGTGCGATTCAATACTATAAAACATTACACACAAACCAAATTATTTTAGGTGCTGTTGGTCAGTTACAA
TTTGAAGTTTTCGAACATAGAATGAAAAACGAATATAATGTTGATGTTGTTATGGAGCCAGTAGGCCGTAAAATTGCACG
TTGGATTGAAAATGAAGACCAAATTACAGATAAGATGAACACATCAAGATCGATTTTAGTGAAAGATAGATATGACGATT
TAGTATTCTTATTTGAAAATGAATTTGCAACAAGATGGTTTGAAGAGAAATTCCTTGAAATTAAATTGTATAGTTTACTT
TAA

Upstream 100 bases:

>100_bases
GTCAGATAGAATACCGTATAGGACACTATTAATGGCCCAGTTTGACCAGTGCCTGTCTATGGGTCAATAGGACTGTGCAA
AAGGTATTGAACCACAAACA

Downstream 100 bases:

>100_bases
CAGCTCAATTGTATAATCGAATTTGTTACATTAAAAATAATTGTTTCGTTGAAGAAAAATAAATTGTATATTTTAAAAGA
AAAAGGTATACTATGATGTA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLN
DDFELEEDHAITNDSAFEQAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKED
VEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQ
FEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFEEKFLEIKLYSLL

Sequences:

>Translated_400_residues
MKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLN
DDFELEEDHAITNDSAFEQAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKED
VEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQ
FEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFEEKFLEIKLYSLL
>Mature_400_residues
MKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLN
DDFELEEDHAITNDSAFEQAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKED
VEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQ
FEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFEEKFLEIKLYSLL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=379, Percent_Identity=27.7044854881267, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI19923640, Length=407, Percent_Identity=22.6044226044226, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI25306283, Length=272, Percent_Identity=26.1029411764706, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=410, Percent_Identity=41.7073170731707, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1789738, Length=351, Percent_Identity=25.3561253561254, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=360, Percent_Identity=25.8333333333333, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323098, Length=350, Percent_Identity=27.1428571428571, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24582462, Length=355, Percent_Identity=25.9154929577465, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 46205; Mature: 46205

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
CCHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHH
DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFEQAIEELMLVEEAGEAFDNDALL
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECC
MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEE
LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
EEECCCEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCHHHHHHHCC
TSRSILVKDRYDDLVFLFENEFATRWFEEKFLEIKLYSLL
CCCEEEEEECCCCEEEEEECHHHHHHHHHHHHEEEEHCCC
>Mature Secondary Structure
MKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
CCHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHH
DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFEQAIEELMLVEEAGEAFDNDALL
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECC
MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHCCEEE
LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
EEECCCEEECCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCHHHHHHHCC
TSRSILVKDRYDDLVFLFENEFATRWFEEKFLEIKLYSLL
CCCEEEEEECCCCEEEEEECHHHHHHHHHHHHEEEEHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA